Serum profile of cytokines interferon gamma and interleukin-10 in ewes subjected to artificial insemination by cervical retraction

Alvares, Caio Tácito Gomes; Cruz, Jurandir Ferreira da; Romano, Carla Cristina; Brandão, F. Z.

doi:10.1016/j.theriogenology.2015.12.009

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…What repercussions do cervical manipulation strategies have? Alvares, Cruz, Romano, and Brandão () confirmed that the Guelph TCAI system does not induce an inflammatory reaction that could compromise the pregnancy. However, repeated attempts to pass the narrow cervical canal (TCAI) with adapted catheters can damage the delicate areas of the cervix, whose cranial segment (3–4 cm) would be very sensitive to manipulations (Álvarez, Chamorro, et al., ).…”

Section: Transcervical Intrauterine Insemination: a Challenge Mid‐waymentioning

confidence: 89%

Current challenges in sheep artificial insemination: A particular insight

Álvarez

Anel-López

Boixo

et al. 2019

Reprod Domestic Animals

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Ovine artificial insemination (OAI) is not commonly performed because of specific problems related to semen application techniques, leading to highly variable results.The ideal methodology (frozen-thawed semen/vaginal route) is unfeasible under field conditions due to the cervix morphology of the ewe, which prevents the process of intrauterine insemination necessary to obtain acceptable results. Currently, OAI commercial programmes use superficial cervical insemination, CAI (vaginal), with chilled semen (15°C) and intrauterine insemination, LAI (laparoscopic), with frozen-thawed semen. The ability to improve upon these contrasting techniques may be derived from examining certain poorly studied factors such as insemination time, productive state of females and alternatives of seminal preservation, some of which we reviewed in this work. This interim solution will remain in use until AI by the vaginal route with frozen-thawed semen is developed, but it poses new challenges in optimizing the freezing of the sperm and adapting the cervical (CAI) and/or transcervical intrauterine AI (TCAI). In this review, we address the current problems and evaluate their methodological (mechanical) and chemical (dilation) alternatives. Currently, TCAI is a methodologically complex technique with poor fertility results, so further studies are needed to improve the logistics of this procedure and the results of its application. K E Y W O R D SAI, cervix, cryopreservation, ovine, sperm, transcervical | 33 ALVAREZ Et AL.

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Section: Transcervical Intrauterine Insemination: a Challenge Mid‐waymentioning

confidence: 89%

Current challenges in sheep artificial insemination: A particular insight

Álvarez

Anel-López

Boixo

et al. 2019

Reprod Domestic Animals

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“…IL-10 is an important anti-inflammatory cytokine in body. The inflammatory reaction caused by surgery trauma will compensatorily increase the secretion of IL-10 [16]. It was found by analyzing the contents of the above inflammatory mediators in patients of the two groups that the contents of IL-1β, IL-2, IL-6, IL-8 and IL-10 in serum of patients in the LS group were significantly lower than those of the laparotomy group, which indicated that the inflammatory reaction and the surgery trauma have a quite slight degree after treated with laparoscopic surgery for acute gastric perforation.…”

Section: Discussionmentioning

confidence: 99%

Curative effect of laparoscopic surgery on acute gastric perforation

Yi-jun¹

2017

JAD

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“…Second, because the time required is relatively long and the penetration success is also variable (2.0-5.2 min; 9.6-66.7%; Casali et al, 2017), which compromises its commercial application and impairs the AI technique, reducing AI fertility rates. Finally, although cervical penetration does not induce an inflammatory response (Alvares et al, 2016), repeated attempts to cross the narrow canal can cause epithelial damage in the most sensitive areas of the cervix (Alvarez et al, 2019).…”

Section: Transcervical Uterine Accessmentioning

confidence: 99%

Strategies to improve cervical relaxation, ovarian response, and embryo yield in sheep undergoing non-surgical embryo recovery

Dias¹

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Multiple ovulation and embryo transfer (MOET) play a key role in accelerating sheep farming, but it is still limited by obstacles such as embryo recovery technique and variability in the superovulatory (SOV) response. This study evaluates alternatives to these limitations, aiming to increase in vivo embryo production in sheep. The first chapter reviews some relevant points of non-surgical embryo recovery (NSER): ovine cervical anatomy, physiological mechanisms of cervical relaxation, and current cervical relaxation protocols (PRC). The second and third chapters evaluate the efficiency of PRC using zero (0.0mg), half (0.5mg), or full dose (1.0mg) of estradiol benzoate (EB) in synchronized and/or superovulated ewes and its effects on NSER feasibility and luteal function. Sheep were randomly assigned to receive 37.5 µg of d-cloprostenol i.m. and 0.0 mg (0EB group; n=12); 0.5 mg (0.5EB group; n=12) or 1.0 mg EB (1.0EB group, n=12) 16 h before and 50 IU oxytocin i.v. 20 minutes before the NSER. In synchronized sheep, NSER was performed in 91.7% (33/36) with a longer transposition duration with Hegar dilator and longer catheter-mandrel (P<0.05) in 0.0EB. The treatments did not differ (P>0.05) in the duration of uterine flushing or the rate of structure recovery. In superovulated ewes, there was no treatment or category effect (P>0.05) concerning NSER success. There was a reduction (P<0.05) in ovarian biometry and progesterone (P4) concentration and an increase in estradiol (E2) concentration on D17. The fourth chapter assesses the effectiveness of hCG administration after ovulation. Ovarian function, P4 profile, and embryo yield were evaluated. Superovulated ewes received 300 IU of hCG i.m. (GhCG, n=24) or not (GControl, n=25) 96 h after P4 removal. NSER efficiency, SOV response, and luteal tissue area were similar between groups (P>0.05). The hCG increased (P<0.05) P4 levels and tended to prevent structural luteolysis (P=0.07) but had no effect (P>0.05) on functional luteolysis. The fifth chapter studies the effect of 250, 333, or 400 IU of a commercial pFSH (1FSH: 1LH) on follicular growth, SOV response, and embryo yield. Superovulated ewes with decreasing doses of 250 (G250), 333 (G333), or 400 IU (G400) of pFSH were submitted to NSER. Estrus response, estrus interval, CL and LUF counts and follicular growth did not differ (P>0.05) between groups. The treatments did not differ (P>0.05) in embryo yield, but G333 and G400 showed a tendency (P=0.06) to have a higher mean of recovered structures compared to G250. This study concludes that: 1) NSER can be successfully performed in different sheep breeds, regardless of category, using a cervical relaxation protocol without estradiol benzoate; 2) the use or not of EB affected the luteal function, interfering mainly in estradiol levels; 3) hCG treatment increased circulating P4 before NSER and may prevent early luteal regression; 4) protocols using 250, 333 and 400 IU of pFSH are efficient in promoting follicular growth and superovulatory responses, but 333 IU appears to be more efficient in increasing embryo yield. Keywords: Biotechnologies. Embryos. MOET. Transcervical.

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Serum profile of cytokines interferon gamma and interleukin-10 in ewes subjected to artificial insemination by cervical retraction

Cited by 4 publications

References 42 publications

Current challenges in sheep artificial insemination: A particular insight

Current challenges in sheep artificial insemination: A particular insight

Curative effect of laparoscopic surgery on acute gastric perforation

Strategies to improve cervical relaxation, ovarian response, and embryo yield in sheep undergoing non-surgical embryo recovery

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