Serum progesterone concentration, volume, and apoptosis of corpora lutea in early, middle and late diestrus in the bitch

Praderio, Romina Gisele; García, María Florencia; Favre, R; Mitacek, María Carla García; Sota, Rodolfo Luzbel de la; Stornelli, María Alejandra

doi:10.1016/j.anireprosci.2020.106591

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…4). As in previous studies, serum P 4 concentration decreased along diestrus (Galabova et (Galabova et al 2003;Stornelli et al 2020). These differences could be due to the different hormonal determination methods used in other studies and the individual differences between bitches.…”

Section: Discussionsupporting

confidence: 83%

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

et al. 2023

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The endometrium is the uterine layer wherein embryo implantation and development take place.Complete knowledge of the endometrium's normal histology is critical for advancing the diagnosis of subclinical endometrial diseases. Several researchers studied subclinical endometritis (END) in different species but the cause/s remain unknown. Furthermore, simple and reliable diagnostic tools are still necessary to diagnose subclinical END. In the bitch, diestrus has been selected as the stage of the reproductive cycle to perform the diagnosis of END. This work aimed to perform morphometric and cytological studies in bitches with normal endometrium (NE) and END in diestrus' stages. Forty-ve mixed-breed clinically healthy bitches, in diestrus, were used in this study. Biopsies and cytology were obtained from uterine horns, processed, and examined using a microscope. Samples from NE and END were selected, images acquired, and software for image analysis was used to measure the endometrial thickness (ET), external glandular diameter (EGD), internal glandular diameter (IGD), glandular density, and glandular cell height (CG). Signi cant differences were observed in GC height, EGD, IGD, and serum P 4 concentrations along diestrus. In END, both EGD and IGD were smaller than NE. The current study showed histomorphometric characteristics that appear to be in uenced by P 4 concentrations. In our opinion, it is relevant to consider how far the bitch had progressed into diestrus at the moment of the diagnosis of endometrial disease. Data recorded in the present study could be helpful to clarify cases of bitches with unexplained infertility or subfertility.

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Section: Discussionsupporting

confidence: 83%

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

et al. 2023

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“…3). As in previous studies, serum P 4 concentration decreased along diestrus (Galabova et al 2003;Stornelli et al 2020). Galabova et al 2003 previously described morphologic changes in canine NE during diestrus.…”

Section: Discussionsupporting

confidence: 82%

Morphometric changes on the endometrium during diestrus stages in bitches with and without histological endometritis

Praderio

Rearte

Mitacek

et al. 2022

Preprint

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The endometrium is a uterine layer essential for achieving optimal embryo implantation and development. Complete knowledge of the endometrium's normal histology is critical for advancing the diagnosis of subclinical endometrial diseases. Several researchers studied subclinical endometritis (END) in different species; however, the cause is unknown, and simple and reliable diagnostic tools are still necessary. In the bitch, diestrus has been selected as the stage of the reproductive cycle to perform diagnostic. This work aimed to perform morphometric and cytological studies in bitches with normal endometrium (NE) and END in the diestrus' different stages. Forty-five mixed-breed bitches, clinically healthy in diestrus, were used in this study. Biopsies and cytology were obtained from uterine horns, processed, and examined using a microscope. Samples from NE and END were selected, photographs were taken, and software for image analysis was used to measure the endometrial thickness (ET), external glandular diameter (EGD), and internal glandular diameter (IGD), glandular density, and glandular cells (GC) height. Significant differences were observed in GC height, EGD, IGD, and serum P4 concentrations along diestrus. In END, EGD and IGD were smaller than NE. The current study showed histomorphometric characteristics that appear to be influenced by P4 concentrations. In our opinion, it is relevant to consider the diestrus stage at the moment of the diagnosis of endometrial disease. Data recorded in the present study could be helpful to bitches with unexplained infertility or subfertility.

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“…Our data indicate that apoptosis, recognized as a crucial process in reproductive phenomena, such as the duration of corpus luteum (Stornelli et al, 2020), may also have a role in the extensive cellular alterations of the CMG and can be regulated in this context by different mechanisms and not exclusively by Caspase-3.…”

Section: Ta B L Ementioning

confidence: 78%

Proliferation and apoptosis dynamics of the normal canine mammary gland during the oestrous cycle evaluated by Ki‐67 and Caspase‐3 indexes

Pinheiro,

Cavalcante,

Lopes

et al. 2024

Reprod Domestic Animals

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Understanding the normal physiology of the canine mammary gland (CMG) is crucial, as it provides a foundational reference for understanding canine mammary neoplasms. The relation between the Proliferation Index (PI) indicated by Ki‐67 expression, along with the Apoptotic Index (AI) determined through Caspase‐3 expression during the oestrous cycle, is inadequately documented in existing literature. This study seeks to offer insights into the interplay between PI and AI in the CMG across oestrous cycle phases. An extensive investigation was conducted on a diverse case series of bitches (n = 18). Oestrous cycle stages were determined through vaginal cytology, histological examination of the reproductive tract and serum progesterone and oestradiol concentrations. The entire mammary chain was histologically examined, and proliferation and apoptosis were assessed via double immunohistochemistry employing anti‐Ki‐67 and Caspase‐3 antibodies. PI and AI were evaluated through a systematic random sampling approach, counting a minimum of 200 cells for each cell type. There was a significantly higher PI during early dioestrus in all mammary gland components, with a greater proportion of positive cells observed in epithelial cells compared to stromal cells. The highest PI was detected in epithelial cells within the end buds. Significant differences were found in Ki‐67 labelling across the cranial mammary glands. A positive and strong correlation was noted between progesterone concentration and PI in epithelial cells. The AI remained consistently low throughout the oestrous cycle, with few differences observed across histological components. Caspase‐3 labelling displayed the highest positivity in caudal mammary pairs. A negative and moderate correlation was identified between progesterone concentration and AI in interlobular mesenchymal cells. This study highlights the influence of endocrine regulation on cell proliferation indices in mammary tissue, emphasizing the need to consider these hormonal variations in toxicopathological studies involving canine mammary gland.

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Serum progesterone concentration, volume, and apoptosis of corpora lutea in early, middle and late diestrus in the bitch

Cited by 4 publications

References 41 publications

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

Morphometric changes on the endometrium during diestrus stages in bitches with and without histological endometritis

Proliferation and apoptosis dynamics of the normal canine mammary gland during the oestrous cycle evaluated by Ki‐67 and Caspase‐3 indexes

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