Serum Prostatic Acid Phosphatase and Cancer of the Prostate

Fishman, William H.; Bonner, Charles D.; Homburger, F.

doi:10.1056/nejm195611152552001

Cited by 73 publications

(16 citation statements)

References 13 publications

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“…PC-3 (human prostatic adenocarcinoma) only shows yellow spots in cytoplasm with few nanofibrils around the cell, indicating low level of ALP expression and agreeing with the association of acid phosphatases with prostate cancer. 34 U-87 MG (human glioma), Capan-2 (human pancreas adenocarcinoma), A375 (human melanoma), and SKOV3 (a drug resistant ovarian carcinoma) exhibit weak fluorescence on their surfaces, indicating low expression level of ALPs. PC-12 Adh (rat pheochromocytoma) has a moderate expression of ALP (about 50% of its surface exhibiting fluorescence).…”

Section: Resultsmentioning

confidence: 99%

Enzyme-Instructed Self-Assembly for Spatiotemporal Profiling of the Activities of Alkaline Phosphatases on Live Cells

Zhou

Berciu

et al. 2016

Chem

156

146

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Summary Alkaline phosphatase (ALP), an ectoenzyme, plays important roles in biology. But there is no activity probes for imaging ALPs in live cell environment due to the diffusion and cytotoxicity of current probes. Here we report the profiling of the activities of ALPs on live cells by enzyme-instructed self-assembly (EISA) of a D-peptidic derivative that forms fluorescent, non-diffusive nanofibrils. Our study reveals the significantly higher activities of ALP on cancer cells than on stromal cells in their co-culture and shows an inherent and dynamic difference in ALP activities between drug sensitive and resistant cancer cells or between cancer cells with and without hormonal stimulation. Being complementary to genomic profiling of cells, EISA, as a reaction-diffusion controlled process, achieves high spatiotemporal resolution for profiling activities of ALPs of live cells at single cell level. The activity probes of ALP contribute to understanding the reversible phosphorylation/dephosphorylation in the extracellular domains that is an emerging frontier in biomedicine.

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Section: Resultsmentioning

confidence: 99%

Enzyme-Instructed Self-Assembly for Spatiotemporal Profiling of the Activities of Alkaline Phosphatases on Live Cells

Zhou

Berciu

et al. 2016

Chem

156

146

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“…Similar reservations apply to the use of tartratelabile AcPase assays, introduced by Abul-Fadl and King (1949) and popularized by Fishman and Lerner (1953) as a means of estimating prostatic AcPase in serum. Claims for the superiority of this assay as a diagnostic procedure for prostatic cancer (Fishman et al, 1956) have not been substantiated (Murphy et al, 1969), and absence of the prostate appears to make no difference to the activity of this enzyme in serum (Dow and Whitaker, 1970).…”

Section: Subjects With Prostatic Cancermentioning

confidence: 99%

“…The conclusions of these authors that raised values occur in about half the cases where metastases are to soft tissues only, and are uncommon when the tumour remains within the prostatic capsule, have been borne out by most authors who have subsequently reported their experience of large series of cases (Fishman, Bonner, and Homburger, 1956; Wood-'A paper presented at the 90th General Meeting of the Association of Clinical Pathologists, Cambridge, 5 April 1973 Received for publication 14 May 197314 May . ard, 1959Veterans Administration Cooperative Urological Research Group, 1968;Murphy, Reynoso, Kenny, and Gaeta, 1969;Prout, 1969;Amador, Price, and Marshall, 1969).…”

mentioning

confidence: 99%

An assessment of serum acid and alkaline phosphatase determinations in prostatic cancer with a clinical validation of an acid phosphatase assay utilizing adenosine 3′ -monophosphate as substrate

Goldberg¹,

Ellis²

1974

J Clin Pathol

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SYNOPSIS Serum acid phosphatase (AcPase) was measured by a colorimetric method utilizing adenosine 3'-monophosphate as substrate in 389 patients. In about half the cases blood was taken shortly after a rectal examination. The upper reference limit (mean + 2SD) for 116 cases with miscellaneous illness after eliminating outliers was 4f1 International Units per litre (U/I) at 37°C, and no correlation existed between AcPase activity and age in these subjects (r = 0 040). Eight of 18 patients with untreated carcinoma confined within the prostate gland had AcPase activities below 4-1 U/1, and all of 27 cases with extension to pelvic soft tissues or to bone exceeded this value. AcPase activities above 4f1 U/l were found in 6% of cases with benign hypertrophy of the prostate, in 5% of cases with non-prostatic cancer, and in none of 22 cases with other urological illness.Raised serum alkaline phosphatase (APase) activity was found in 60% of patients with untreated prostatic cancer and in only 6% of patients free of prostatic cancer, in most of whom there was a clinical explanation for the elevation. The correlation between the two phosphatase activities was not significant (r = 0.294). While APase activity does not reflect the stage of the disease as closely as AcPase activity, and is not so frequently elevated, it provided useful confirmation of the diagnosis in five patients of the present series whose AcPase levels were normal or only minimally elevated.

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“…The technic, described initially by Ackerman and W angensteen at the University of Minnesota, is based on the observation that P 3~ localizes FIGURE 10. Balloon in place for radioautogram of the stomach (Ackerman and Wangensteen).…”

Section: Over-all Reaulta Of Treatmentmentioning

confidence: 99%

“…6 Measurement of the "prostatic" fraction of the serum acid phosphatase has been suggested in an effort to improve the diagnostic value of determinations of phosphatase. 10 On the basis that prostatic acid phosphatase is selectively inhibited by !-tartrate, Cook, Fishman and Clarke 11 measured the serum prostatic acid phosphatase in patients with prostatic carcinoma and considered these determinations to be of more value than determination of the total serum acid phosphatase. Increases of the prostatic fraction above the normal value of 0.6 unit (King and Armstrong) did not occur in patients whose carcinoma was confined to the prostate gland.…”

Section: Corroborative Evidencementioning

confidence: 99%

Panel on Cancer Detection

1963

Postgraduate Medicine

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Serum Prostatic Acid Phosphatase and Cancer of the Prostate

Cited by 73 publications

References 13 publications

Enzyme-Instructed Self-Assembly for Spatiotemporal Profiling of the Activities of Alkaline Phosphatases on Live Cells

Enzyme-Instructed Self-Assembly for Spatiotemporal Profiling of the Activities of Alkaline Phosphatases on Live Cells

An assessment of serum acid and alkaline phosphatase determinations in prostatic cancer with a clinical validation of an acid phosphatase assay utilizing adenosine 3′ -monophosphate as substrate

Panel on Cancer Detection

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