Serum protein adsorption and platelet adhesion on pluronic™-adsorbed polysulfone membranes

Higuchi, Akon; Sugiyama, Kaichiro; Yoon, Boo Ok; Sakurai, Masaru; Hara, Mariko; Sumita, Masaya; Sugawara, Shu Ichi; Shirai, Toshikazu

doi:10.1016/s0142-9612(03)00186-8

Cited by 182 publications

(125 citation statements)

References 26 publications

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“…Newer advances in gene delivery are summarized elsewhere [55]. Pluronics® represent a bio-inert environment, attributed by the hydrophilicity and flexibility of PEO chains [56]. Therefore, most cells do not grow on these polymers, which have been used as tissue adhesion barriers.…”

Section: Peo/ppo-based Systemsmentioning

confidence: 99%

Thermoresponsive hydrogels in biomedical applications

Klouda

Mikos

2008

European Journal of Pharmaceutics and Biopharmaceutics

1,134

696

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Environmentally responsive hydrogels have the ability to turn from solution to gel when a specific stimulus is applied. Thermoresponsive hydrogels utilize temperature change as the trigger that determines their gelling behavior without any additional external factor. These hydrogels have been interesting for biomedical uses as they can swell in situ under physiological conditions and provide the advantage of convenient administration. The scope of this paper is to review the aqueous polymer solutions that exhibit transition to gel upon temperature change. Typically, aqueous solutions of hydrogels used in biomedical applications are liquid at ambient temperature and gel at physiological temperature. The review focuses mainly on hydrogels based on natural polymers, N-isopropylacrylamide polymers, poly(ethylene oxide)-b-poly(propylene oxide)-bpoly(ethylene oxide) polymers as well as poly(ethylene glycol)-biodegradable polyester copolymers.

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Section: Peo/ppo-based Systemsmentioning

confidence: 99%

Thermoresponsive hydrogels in biomedical applications

Klouda

Mikos

2008

European Journal of Pharmaceutics and Biopharmaceutics

1,134

696

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“…A wide range of substances have been used as blocking agents, with different surfaces being blocked effectively by only certain compounds, depending upon the relative chemical properties of surface and blocker. To establish a suitable blocking protocol for these PDMS microcolumns, a series of potential blockers with varying properties were tested: BSA (a single purified protein) and non-fat milk powder (predominately a mixture of proteins and carbohydrates), both of which are widely used for blocking ELISAs, microarrays and western blots, 36 Pluronic F-68 (a triblock PEO-PPO-PEO copolymer previously reported to suppress protein adsorption 37 ) and DDM (a non-ionic detergent recently reported to effectively block native PDMS that was doped with phospholipids 7 ). Fluorescently labelled streptavidin was employed as a test protein to assess the efficiency of these blockers.…”

Section: Blocking Of Potential Nsa Sitesmentioning

confidence: 99%

On-chip immunoprecipitation for protein purification

Sandison¹,

Cumming²,

Kölch³

et al. 2010

Lab Chip

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Immunoprecipitation (IP) is one of the most widely used and selective techniques for protein purification. Here, a miniaturised, polymer-supported immunoprecipitation (mIP) method for the onchip purification of proteins from complex mixtures is described. A 4 ml PDMS column functionalised with covalently bound antibodies was created and all critical aspects of the mIP protocol (antibody immobilisation, blocking of potential non-specific adsorption sites, sample incubation and washing conditions) were assessed and optimised. The optimised mIP method was used to obtain purified fractions of affinity-tagged protein from a bacterial lysate.

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“…Researches have shown that Pluronic surfactants can considerably reduce protein adsorption [25,29] and cell adhesion. [21,25,30] In addition to Pluronic family, the other surfactant candidate, Tween 20 (polyoxyethilene sorbitan monolaurate), has been proven to resist protein adsorption. [31] Similar to the Pluronic surfactants, a Tween 20 coating can be simply prepared by physical adsorption on to a surface.…”

mentioning

confidence: 99%

Simple poly(dimethylsiloxane) surface modification to control cell adhesion

2008

Surface & Interface Analysis

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Poly(dimethylsiloxane) (PDMS) has a long history of exploitation in a variety of biological and medical applications. Particularly in the past decade, PDMS has attracted interest as a material for the fabrication of microfluidic biochip. The control of cell adhesion on a PDMS surface is important in many microfluidic applications such as cell culture or cell-based chemicals/drug testing. Unlike many complicated approaches, this study reports simple methods of PDMS surface modification to effectively inhibit or conversely enhance cell adhesion on a PDMS surface using Pluronic surfactant solution and poly-L-lysine, respectively. This research basically succeeded our prior work to further confirm the long-term capability of 3% Pluronic F68 surfactant to suppress cell adhesion on a PDMS surface over a 6-day cell culture. Microscopic observation showed that the treated PDMS surface created an unfavorable interface, where chondrocytes seemed to clump together on day 2 and 6 after chondrocyte seeding, and there was no sign of chondrocyte spreading. On the opposite side, results demonstrated that the poly-L-lysinetreated surface significantly increased fibroblast adhesion by 32% in contrast to the untreated PDMS, which is comparable to the commercial cell-culture-grade microplate. However, fibronectin treatment did not have such an effect. All these fundamental information is found useful for any PDMS-related application.

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Serum protein adsorption and platelet adhesion on pluronic™-adsorbed polysulfone membranes

Cited by 182 publications

References 26 publications

Thermoresponsive hydrogels in biomedical applications

Thermoresponsive hydrogels in biomedical applications

On-chip immunoprecipitation for protein purification

Simple poly(dimethylsiloxane) surface modification to control cell adhesion

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