Serum Protein Electrophoresis in Acrylamide Gel: Patterns from Normal Human Subjects

Abstract

Technical improvements in the method for vertical acrylamide gel electrophoresis (Raymond) have resulted in improved resolution and reproducibility. In a survey of approximately 200 normal human serums, ten lines were found to be common in all specimens. Interposed with these constant lines and in addition to known variations in the pattern reflecting haptoglobin and transferrin types, at least one line followed that of albumin and three followed that of transferrin; the presence or absence of these varied from individual to individual. Based on these variations, the electrophoretic patterns of normal humans may be divided into at least 12 discrete groups.

Morphology and Genetics of the Ciscoes (Actinopterygii: Salmoniformes: Salmonidae: Coregoninae: Coregonus) from the Solovetsky Archipelago (White Sea) as a Key to Determination of the Taxonomic Position of Ciscoes in Northeastern Europe

“…Vertical polyacrylamide gel electrophoresis was performed in 7.5% polyacrylamide using Tris-Borate-EDTA (TBE) buffer. Gel, stock solutions and Tris-Borate-EDTA (TBE) buffer were prepared according Peacock et al (1965). Voltage was applied for 20 min before addition of the samples.…”

Polyploid races, genetic structure and morphological features of the earthworm Octodrіlus transpadanus (Rosa, 1884) (Oligohaeta: Lumbricidae) in the Ukraine

“…Electrophoretic variability of spectra of enzymes (aspartate aminotransferase (AST), malate dehydrogenase (MDH), nonspecifi c esterases (ES) and superoxide dismutase (SOD) in extracts from a caudal part of body was investigated by the method of PAG-electrophoresis in Tris-EDTA-borate buffer system (Peacock et al, 1965) .…”

Features of the genetic structure of the earthworms Aporrectodea (Superspecies) Caliginosa (Oligochaeta: Lumbricidae) complex in Ukraine

“…A total of 21 samples which included 646 individuals obtained from the following locations were analyzed: Nikolaevka, Simferopol District (Crimea Autonomous Republic) ( The electrophoretic variability of the spectra of three enzymes which are encoded by six loci was studied by means of electrophoresis in polyacrylamide gel using tris-EDTA-borate (pH 8.5) from the system of buffers of [16] in tissue extracts taken from the tail of the body of the animal.…”