Serum protein-hyaluronic acid complex nanocarriers: Structural characterisation and encapsulation possibilities

Kovács, Alexandra N.; Varga, Norbert; Juhász, Ádám; Csapó, Edit

doi:10.1016/j.carbpol.2020.117047

Cited by 29 publications

(16 citation statements)

References 31 publications

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“…Recently, the development of organic-based nanocarriers has been the focus of extensive research thanks to their outstanding biocompatible and biodegradable properties [ 1 ]. Besides biocompatible polymers [ 6 , 7 , 8 , 9 , 10 ], serum albumins [ 11 , 12 , 13 ] can be also used to synthesize several biocompatible drug delivery systems applying the layer-by-layer (LbL) preparation technique. The liposomes (LIPs) are also potential carriers due to the amphiphilic properties of the phospholipid building blocks [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

The pH-Dependent Controlled Release of Encapsulated Vitamin B1 from Liposomal Nanocarrier

Juhász

Ungor

Várkonyi

et al. 2021

IJMS

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In this work, we firstly presented a simple encapsulation method to prepare thiamine hydrochloride (vitamin B1)-loaded asolectin-based liposomes with average hydrodynamic diameter of ca. 225 and 245 nm under physiological and acidic conditions, respectively. In addition to the optimization of the sonication and magnetic stirring times used for size regulation, the effect of the concentrations of both asolectin carrier and initial vitamin B1 on the entrapment efficiency (EE %) was also investigated. Thermoanalytical measurements clearly demonstrated that after the successful encapsulation, only weak interactions were discovered between the carriers and the drug molecules. Moreover, the dissolution profiles under physiological (pH = 7.40) and gastric conditions (pH = 1.50) were also registered and the release profiles of our liposomal B1 system were compared with the dissolution profile of the pure drug solution and a manufactured tablet containing thiamin hydrochloride as active ingredient. The release curves were evaluated by nonlinear fitting of six different kinetic models. The best goodness of fit, where the correlation coefficients in the case of all three systems were larger than 0.98, was reached by application of the well-known second-order kinetic model. Based on the evaluation, it was estimated that our liposomal nanocarrier system shows 4.5-fold and 1.5-fold larger drug retention compared to the unpackaged vitamin B1 under physiological conditions and in artificial gastric juice, respectively.

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Section: Introductionmentioning

confidence: 99%

The pH-Dependent Controlled Release of Encapsulated Vitamin B1 from Liposomal Nanocarrier

Juhász

Ungor

Várkonyi

et al. 2021

IJMS

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“…based on the reactive groups such as the amine group and carboxyl group existing in the structure of these polysaccharides. , It is difficult for polysaccharides owing to their hydrophilicity to form into micro-/nanoparticles without the participation of other substances. Therefore, based on the natural-born highly charged property derived from amine or carboxyl groups, the noncovalent interaction such as the electrostatic interaction between polysaccharides such as chitosan (positively charged) or hyaluronic acid (negatively charged) and proteins is the most used mechanism to load target drugs. , Besides that, through the chemical conjugation of a hydrophobic segment for improved amphiphilicity, modified polysaccharides could form into micelles in aquatic conditions and hence load target protein/peptide therapeutics via hydrophobic interaction.…”

Section: Polymer-based Systemsmentioning

confidence: 99%

Sustained Release Systems for Delivery of Therapeutic Peptide/Protein

et al. 2021

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Peptide/protein therapeutics have been significantly applied in the clinical treatment of various diseases such as cancer, diabetes, etc. owing to their high biocompatibility, specificity, and therapeutic efficacy. However, due to their immunogenicity, instability stemming from its complex tertiary and quaternary structure, vulnerability to enzyme degradation, and rapid renal clearance, the clinical application of protein/peptide therapeutics is significantly confined. Though nanotechnology has been demonstrated to prevent enzyme degradation of the protein therapeutics and thus enhance the half-life, issues such as initial burst release and uncontrollable release kinetics are still unsolved. Moreover, the traditional administration method results in poor patient compliance, limiting the clinical application of protein/peptide therapeutics. Exploiting the sustained-release formulations for more controllable delivery of protein/peptide therapeutics to decrease the frequency of injection and enhance patient compliance is thus greatly meaningful. In this review, we comprehensively summarize the substantial advancements of protein/peptide sustainedrelease systems in the past decades. In addition, the advantages and disadvantages of all these sustained-release systems in clinical application together with their future challenges are also discussed in this review.

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“…Polysaccharides such as chitosan (Chit) and hyaluronan (HyA) are promising materials in this field due to their advantageous characteristics [1,4,5]. Accordingly, they are used in several forms (hydrogels, films, and nanoparticles (NPs)) and applications [2,[6][7][8]. The precise combination of these oppositely charged polysaccharides can produce finely tuned NPs, which can be used for gene delivery, or for loading different drugs and cancer therapeutics [9,10].…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Labeling of Hyaluronic Acid-Chitosan Nanocarriers by Protein-Stabilized Gold Nanoclusters

2020

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In medical research the visualization of drug carrier accumulation and release of the loaded drugs in vivo is an important field. In this work, two protein-stabilized gold nanoclusters (Au NCs) as effective fluorescent reporters (FRs) were investigated for labeling of biocompatible chitosan-modified hyaluronic acid based nanocarriers having two different structures. The colloid stability of the labeled carriers was studied by dynamic light scattering and Zeta potential measurements, while the changes in the fluorescence of the lysozyme- (LYZ) and bovine serum albumin (BSA)-stabilized Au NCs were analyzed by spectrofluorimetry and confocal fluorescent microscopy. We found that the labeling was effective with a wide range of marker:carrier mass ratios, and the fluorescence of the NCs and the colloid stability of the complexes were retained. Labeling during preparation and subsequent labeling were compared, and based on composition (nanocluster:carrier mass ratio) and structure of the complex systems we preferred the latter method, as it left the Au NCs free for further modifications. Considering both marker:carrier mass ratios and emission intensities, the LYZ-stabilized Au NCs proved to be better labels. The core-shell type carrier formulations showed increased fluorescence with LYZ-stabilized NCs, presumably from aggregation induced emission.

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Serum protein-hyaluronic acid complex nanocarriers: Structural characterisation and encapsulation possibilities

Cited by 29 publications

References 31 publications

The pH-Dependent Controlled Release of Encapsulated Vitamin B1 from Liposomal Nanocarrier

The pH-Dependent Controlled Release of Encapsulated Vitamin B1 from Liposomal Nanocarrier

Sustained Release Systems for Delivery of Therapeutic Peptide/Protein

Fluorescent Labeling of Hyaluronic Acid-Chitosan Nanocarriers by Protein-Stabilized Gold Nanoclusters

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