Serum protein S100A9, SOD3, and MMP9 as new diagnostic biomarkers for pulmonary tuberculosis by iTRAQ‐coupled two‐dimensional LC‐MS/MS

Xu, Dandan; Li, Yanyuan; Li, Xiang; Wei, Li-Liang; Pan, Zhifen; Jiang, Tingting; Chen, Zhongliang; Wang, Chong; Cao, Wei; Zhang, Xing; Ping, Zepeng; Liu, Changming; Liu, Jiyan; Li, Zhongjie; Li, Jicheng

doi:10.1002/pmic.201400366

Cited by 67 publications

(77 citation statements)

References 46 publications

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“…We also found that protein S100-A9, extracellular superoxide dismutase [Cu-Zn], and matrix metalloproteinase 9 may represent potential serological markers for TB to distinguish patients with this disorder from healthy controls or those with pneumonia or lung cancer patients [19]. However, the serum protein levels were not ascertained in differential diagnosis groups such as patients with COPD or treated TB cases in either study, and the latter analysis was conducted on only a small number of patients.…”

Section: Discussionmentioning

confidence: 99%

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Shi

Wei

et al. 2017

PLoS ONE

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The aim of this study was to discover novel biomarkers for pulmonary tuberculosis (TB). Differentially expressed proteins in the serum of patients with TB were screened and identified by iTRAQ-two dimensional liquid chromatography tandem mass spectrometry analysis. A total of 79 abnormal proteins were discovered in patients with TB compared with healthy controls. Of these, significant differences were observed in 47 abnormally expressed proteins between patients with TB or pneumonia and chronic obstructive pulmonary disease (COPD). Patients with TB (n = 136) exhibited significantly higher levels of serum amyloid A (SAA), vitamin K-dependent protein Z (PROZ), and C4b-binding protein β chain (C4BPB) than those in healthy controls (n = 66) (P<0.0001 for each) albeit significantly lower levels compared with those in patients with pneumonia (n = 72) (P<0.0001 for each) or COPD (n = 72) (P<0.0001, P<0.0001, P = 0.0016, respectively). After 6 months of treatment, the levels of SAA and PROZ were significantly increased (P = 0.022, P<0.0001, respectively), whereas the level of C4BPB was significantly decreased (P = 0.0038) in treated TB cases (n = 72). Clinical analysis showed that there were significant differences in blood clotting and lipid indices in patients with TB compared with healthy controls, patients with pneumonia or COPD, and treated TB cases (P<0.05). Correlation analysis revealed significant correlations between PROZ and INR (rs = 0.414, P = 0.044), and between C4BPB and FIB (rs = 0.617, P = 0.0002) in patients with TB. Receiver operating characteristic curve analysis revealed that the area under the curve value of the diagnostic model combining SAA, PROZ, and C4BPB to discriminate the TB group from the healthy control, pneumonia, COPD, and cured TB groups was 0.972, 0.928, 0.957, and 0.969, respectively. Together, these results suggested that SAA, PROZ, and C4BPB may serve as new potential biomarkers for TB. Our study may thus provide experimental data for the differential diagnosis of TB.

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Section: Discussionmentioning

confidence: 99%

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Shi

Wei

et al. 2017

PLoS ONE

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“…Bacterial studies include iTRAQ studies of pulmonary tuberculosis (PTB) in humans and mycobacterial infections in cattle . In the works of Xu et al and Wang et al, differences in serum proteome were studied in association with PTB . Included in these was the use of a pooled iTRAQ strategy to compare treated, untreated and cured PTB patients with healthy controls (250 samples in total), in which markers of cured PTB were identified .…”

Section: Applications Of Isobaric Labeling For Serum and Plasma Protementioning

confidence: 99%

Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

Moulder

Bhosale

Goodlett

et al. 2017

Mass Spectrometry Reviews

128

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Over the past decade, chemical labeling with isobaric tandem mass tags, such as isobaric tags for relative and absolute quantification reagents (iTRAQ) and tandem mass tag (TMT) reagents, has been employed in a wide range of different clinically orientated serum and plasma proteomics studies. In this review the scope of these works is presented with attention to the areas of research, methods employed and performance limitations. These applications have covered a wide range of diseases, disorders and infections, and have implemented a variety of different preparative and mass spectrometric approaches. In contrast to earlier works, which struggled to quantify more than a few hundred proteins, increasingly these studies have provided deeper insight into the plasma proteome extending the numbers of quantified proteins to over a thousand.

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“…Markel et al (13) reported that CEACAM1 and MICA could serve as novel serum biomarkers in patients with acute and recurrent pericarditis. Xu et al (14) demonstrated that the candidate serum biomarkers (S100A9, SOD3 and MMP9) performed high sensitivity and specificity in discriminating pulmonary tuberculosis. These findings indicate that serum protein biomarkers serve an important role in the prediction and diagnosis of diseases.…”

Section: Introductionmentioning

confidence: 99%

A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients

Sun

Wan

et al. 2017

Molecular Medicine Reports

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Ovarian hyperstimulation syndrome (OHSS) is a potentially life-threatening, iatrogenic complication that occurs during assisted reproduction. Polycystic ovarian syndrome (PCOS) significantly increases the risk of OHSS during controlled ovarian stimulation. Therefore, a more effective early prediction technique is required in PCOS patients. Quantitative proteomic analysis of serum proteins indicates the potential diagnostic value for disease. In the present study, the authors revealed the differentially expressed proteins in OHSS patients with PCOS as new diagnostic biomarkers. The promising proteins obtained from liquid chromatography-mass spectrometry were subjected to ELISA and western blotting assay for further confirmation. A total of 57 proteins were identified with significant difference, of which 29 proteins were upregulated and 28 proteins were downregulated in OHSS patients. Haptoglobin, fibrinogen and lipoprotein lipase were selected as candidate biomarkers. Receiver operating characteristic curve analysis demonstrated all three proteins may have potential as biomarkers to discriminate OHSS in PCOS patients. Haptoglobin, fibrinogen and lipoprotein lipase have never been reported as a predictive marker of OHSS in PCOS patients, and their potential roles in OHSS occurrence deserve further studies. The proteomic results reported in the present study may gain deeper insights into the pathophysiology of OHSS.

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Serum protein S100A9, SOD3, and MMP9 as new diagnostic biomarkers for pulmonary tuberculosis by iTRAQ‐coupled two‐dimensional LC‐MS/MS

Cited by 67 publications

References 46 publications

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients

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