Serum proteome profiling of metastatic breast cancer using recombinant antibody microarrays

Carlsson, Anders; Wingren, Christer; Ingvarsson, Johan; Ellmark, Peter; Baldertorp, Bo; Fernö, Mårten; Olsson, Håkan; Borrebaeck, Carl

doi:10.1016/j.ejca.2007.11.025

Cited by 101 publications

(108 citation statements)

References 43 publications

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“…These molecules modulate growth, proliferative, migration, and apoptotic pathways, often resulting in metastasis and advanced stage disease (33)(34)(35)(36)(37)(38)(39)(40). Next, we evaluated expression of a panel of cytokines associated with disease progression using pancreas tumor tissues from the same cohort in the previous section by qPCR.…”

Section: Resultsmentioning

confidence: 99%

Inactivation of the Transcription Factor GLI1 Accelerates Pancreatic Cancer Progression

Mills

Zhang²,

Marler

et al. 2014

Journal of Biological Chemistry

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Background: GLI1 is required for pancreatic tumor initiation; however, its role at later stages of carcinogenesis remains elusive. Results: Genetic inactivation of GLI1 accelerates pancreatic cancer progression. Conclusion: GLI1 can act as both a promoter and suppressor of pancreatic carcinogenesis depending on the tumor stage. Significance: This knowledge increases our understanding of pancreatic carcinogenesis and may help the design of therapies targeting GLI1-related pathways.

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Section: Resultsmentioning

confidence: 99%

Inactivation of the Transcription Factor GLI1 Accelerates Pancreatic Cancer Progression

Mills

Zhang²,

Marler

et al. 2014

Journal of Biological Chemistry

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“…However, the lack of validated antibodies to most human proteins (10) makes it impossible to use antibody-based protocols for a majority of the potential protein targets, and this is even more difficult for assays based on paired antibodies that require two distinct antibodies with separate and non-overlapping epitopes. Because of this limitation, current studies are directed by candidate target lists reported in the literature (11) or in associated gene expression studies (12) or built on collections of in-house binder libraries (13).…”

mentioning

confidence: 99%

Toward Next Generation Plasma Profiling via Heat-induced Epitope Retrieval and Array-based Assays

Schwenk

Igel

Neiman

et al. 2010

Molecular & Cellular Proteomics

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There is a need for high throughput methods for screening patient samples in the quest for potential biomarkers for diagnostics and patient care. Here, we used a combination of undirected target selection, antibody suspension bead arrays, and heat-induced epitope retrieval to allow for protein profiling of human plasma in a novel and systematic manner. Several antibodies were found to reveal altered protein profiles upon epitope retrieval at elevated temperatures with limits of detection improving into lower ng/ml ranges. In a study based on prostate cancer patients, several proteins with differential profiles were discovered and subsequently validated in an independent cohort. For one of the potential biomarkers, the human carnosine dipeptidase 1 protein (CNDP1), the differences were determined to be related to the glycosylation status of the targeted protein. The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format. Molecular & Cellular Proteomics 9: 2497-2507, 2010.

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“…Studying the serum proteome from metastatic breast cancer patients and healthy controls with recombinant single-chain variable fragment (scFv) microarrays [59], breast cancer was identified with a specificity and sensitivity of 85% on the basis of 129 serum analytes.…”

Section: Oncogenomics and Cancer Proteomicsnovel Approaches In Biomarmentioning

confidence: 99%

Phosphoproteomics for the Mapping of Altered Cell Signaling Networks in Breast Cancer

Villamar-Cruz¹,

Arias-Romero²

2013

Oncogenomics and Cancer Proteomics - Novel Approaches in Biomarkers Discovery and Therapeutic Targets in Cancer

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Serum proteome profiling of metastatic breast cancer using recombinant antibody microarrays

Cited by 101 publications

References 43 publications

Inactivation of the Transcription Factor GLI1 Accelerates Pancreatic Cancer Progression

Inactivation of the Transcription Factor GLI1 Accelerates Pancreatic Cancer Progression

Toward Next Generation Plasma Profiling via Heat-induced Epitope Retrieval and Array-based Assays

Phosphoproteomics for the Mapping of Altered Cell Signaling Networks in Breast Cancer

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