Serum sE‐selectin levels and carcinoembryonic antigen mRNA‐expressing cells in peripheral blood as prognostic factors in colorectal cancer patients

Abstract

BACKGROUND: This study analyzed the possible prognostic value of presurgical serum soluble (s)E-selectin levels and/or carcinoembryonic antigen (CEA) mRNA positivity in predicting the disease-free survival of colorectal cancer (CRC) patients. METHODS: CEA mRNA (obtained from blood-borne cells by reverse transcriptase-polymerase chain reaction [RT-PCR]), tumor necrosis factor-a (TNF-a), and sE-selectin levels were analyzed in blood samples obtained from 78 patients with primary (n ¼ 62) or recurrent (n ¼ 16) CRC, 40 patients with benign colorectal (CR) diseases, and 78 controls. RESULTS: CEA mRNA positivity by RT-PCR was significantly associated with advanced stage (P < .05). Median baseline sE-selectin levels were higher in patients with CRC (43 ng/mL) compared with controls (36 ng/mL) or patients with benign CR diseases (31 ng/mL, P < .001). These were significantly associated with CEA mRNA positivity by RT-PCR (P < .05). Multivariate analysis by forward stepping showed that elevated TNF-a (P ¼ .001) and CEA mRNA positivity by RT-PCR (P ¼ .0001) were independent predictors of elevated baseline sE-selectin levels. Positive presurgical sE-selectin levels were associated with an increased recurrence rate compared with patients with low levels of this molecule (P < .001). Positivity for both CEA mRNA and sE-selectin had a negative prognostic impact, with a 5-year recurrence-free survival rate of 51% compared with 95% of patients with negative parameters (P < .05). CONCLUSIONS: Detection of presurgical serum sE-selectin levels and CEA mRNA-positive blood-borne cells in CRC patients might provide useful prognostic information in terms of recurrence-free survival, either alone or in combination, and may help in the choice of more aggressive treatment and/or more strict follow-up procedures in high-risk patients.

Pathologic evaluation of tumor-associated macrophage density and vessel inflammation in invasive breast carcinomas

“…Thus, increased expression levels of sE-selectin were associated with reduced overall survival in breast cancer (49). Similarly, pre-surgical sE-selectin expression levels were higher in patients with colorectal cancer (43 ng/ml) compared with patients with benign diseases (43 ng vs. 31 ng/ml) and were positively associated with carcinoembryonic antigen tumor marker and poorer prognosis (both P<0.001) (50). However, a study of microarray data from 1,809 breast cancer patients with no previous treatment history revealed that E-selectin expression level was associated with longer survival times (HR, 0.67; CI 95%, 0.54–0.83; P=0.001) (51).…”

Expression of growth-regulated oncogene-1, hepatocyte growth factor, platelet-derived growth factor-AA and soluble E-selectin and their association with high-risk human papillomavirus infection in squamous cell carcinoma of the uterine cervix

“…Previous studies have demonstrated that sE-selectin is highly expressed in colorectal cancer and is closely associated with the progression, recurrence and metastasis of cancer (31,32). It was observed that the serum levels of sE-selectin were significantly increased in CSCC patients and were correlated with FIGO stage and tumor size.…”

“…Cheng et al (42) reported that GRO-1 may be a promising adverse prognostic molecular marker, and Miyake et al (43) confirmed the poor prognostic value of GRO-1 in bladder cancer. Numerous studies have demonstrated the prognostic value of sE-selectin in colorectal cancer (31,34). Our findings suggested that GRO-1 and sE-selectin may have an impact on the prognosis of CSCC patients by affecting FIGO stage or lymph node metastasis, thus GRO-1 and sE-selectin were not independent prognostic risk factors.…”

Phase I Study of Pazopanib and Ixabepilone in Patients With Solid Tumors

“…[18][19][20][21] High presurgical serum levels of sE-selectin correlate with increased recurrence and poor survival in colon cancer. 22,23 It can be an independent prognostic factor for patients with advanced lung cancer. 24 Although it is difficult to draw solid conclusions due to the fact that we compared the changes in cytokine levels across different dose levels, our findings suggest that serum sE-selectin may be explored as a potential biomarker for monitoring response to chemotherapy.…”