Serum sFlt-1/PlGF ratio has better diagnostic ability in early- compared to late-onset pre-eclampsia

Chuah, Theng Theng; Tey, Wan Shi; Ng, Mor Jack; Tan, Edward T. H.; Chern, Bernard; Tan, Kok Hian

doi:10.1515/jpm-2017-0288

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…It has been suggested that in EOPE poor placentation plays a dominant role, whereas in LOPE inflammatory processes are involved [1]. Studies have shown that PlGF levels are lower and have better diagnostic ability in EOPE than LOPE, which underlines our finding of PlGF DNA hypomethylation in EOPE but not in LOPE [36, 37].…”

Section: Discussionsupporting

confidence: 51%

Lower S-adenosylmethionine levels and DNA hypomethylation of placental growth factor (PlGF) in placental tissue of early-onset preeclampsia-complicated pregnancies

et al. 2019

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IntroductionThe pathophysiology of preeclampsia is largely unknown. Serum placental induced growth factor (PlGF) levels are decreased during second trimester pregnancy. Aberrant DNA methylation is suggested to be involved in the etiology of preeclampsia (PE). We hypothesize that DNA methylation is altered in PE placentas determined the methylation index by measuring placental S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) levels. In addition, we assessed global DNA methylation status by long-interspersed nuclear element-1 (LINE-1) and DNA methylation status of the PlGF gene.MethodsPlacental tissue of 11 early onset PE (EOPE), 11 late onset PE (LOPE) and 60 controls consisting of 25 uncomplicated controls 20 fetal growth restriction (FGR) and 15 preterm births (PTB) controls was collected from a nested case-control study of The Rotterdam Periconceptional Cohort. RNA and DNA was isolated from placental tissue and DNA was treated with sodium bisulfite. SAM and SAH levels were measured by LC-ESI-MS/MS. Methylation of LINE-1 and PlGF genes was analyzed by Sequenom Epityper and. mRNA expression of PlGF was assessed with qPCR. Differences were assessed by analysis of covariance (ANCOVA) corrected for gestational age and birth weight.ResultsPlacental SAM levels were significantly lower in placental tissue of EOPE pregnancies compared to PTB controls (mean difference -240 ± 71.4 nmol/g protein, P = 0.01). PlGF DNA methylation was decreased in placental tissue of EOPE cases versus LOPE (mean difference -17.4 ± 5.1%, P = 0.01), uncomplicated controls (mean difference -23.4 ± 5.4%%, P <0.001), FGR controls (mean difference -17.9 ± 4.6%, P = 0.002) and PTB controls (mean difference -11.3 ± 3.8% P = 0.04). No significant differences were observed in SAH, SAM:SAH ratio, LINE-1 DNA methylation and PlGF mRNA expression between groups.DiscussionThe hypomethylation state of the placenta in EOPE, which is reflected by lower SAM and PlGF DNA hypomethylation underlines the possible role of placental DNA hypomethylation in the pathophysiology of EOPE, which needs further investigation.

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Section: Discussionsupporting

confidence: 51%

Lower S-adenosylmethionine levels and DNA hypomethylation of placental growth factor (PlGF) in placental tissue of early-onset preeclampsia-complicated pregnancies

et al. 2019

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“…There were two reports of PlGF alone predicting LO-PE 50,55 , the combined DOR was 8.572 (95% CI: 2.254-32.603). sFlt-1/PlGF alone predicted EO-PE in 12 cases 33,37,50,55,65,79,81,83 , the combined DOR was 230.24 (95% CI: 63.956-828.82), and the AUC was 0.9806. There were seven data points that predicted LO-PE 50,55,65,79,81 , the combined DOR was 20.997 (95% CI: 5.947-74.132), and the AUC was 0.8877.…”

Section: Subgroup Analysis and Metaregressionmentioning

confidence: 97%

“…sFlt-1/PlGF alone predicted EO-PE in 12 cases 33,37,50,55,65,79,81,83 , the combined DOR was 230.24 (95% CI: 63.956-828.82), and the AUC was 0.9806. There were seven data points that predicted LO-PE 50,55,65,79,81 , the combined DOR was 20.997 (95% CI: 5.947-74.132), and the AUC was 0.8877. The results are shown in Figures S8-S13 and Table S3.…”

Section: Subgroup Analysis and Metaregressionmentioning

confidence: 97%

Predictive Performance of sFlt-1, PlGF and the sFlt-1/PlGF Ratio for Preeclampsia: A Systematic Review and Meta-Analysis

Ding

2024

Preprint

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Background: It is difficult to evaluate whether monitoring serum sFlt-1, PlGF, or sFlt-1/PlGF in pregnant women who are suspected of having PE can significantly shorten the PE diagnosis time. Objectives: To estimate the accuracy of sFlt-1, PlGF and sFlt-1/PlGF in preeclampsia prediction. Search Strategy: Databases including PubMed, Web of Science, Medline, CNKI, SinoMed, VIP Journal, and Wanfang Data were searched for eligible studies published until October 7, 2020. Selection Criteria: The research subjects were pregnant women with or without PE. The research types were case-control studies and cohort studies. This was an original study involving the detection of at least one of the following in the blood, serum or plasma: sFlt-1, PlGF, and sFlt-1/PlGF. Data Collection and Analysis: Meta-Disc 1.4 was employed, using the Sen, Spe, PLR, NLR, and DOR to plot SROC, and subgroup analysis and meta-regression were conducted. Main Results: Meta-analysis showed that for sFlt, PlGF and sFlt-1/PlGF, the Sen was 0.811 (95% CI: 0.783-0.837), 0.735 (95% CI: 0.713–0.757), and 0.779 (95% CI: 0.763–0.795), respectively; the Spe was 0.786 (95% CI: 0.769-0.802), 0.731 (95% CI: 0.721-0.741),and 0.885 (95% CI: 0.881-0.889), respectively. Conclusions: The sFlt-1/PlGF ratio showed better predictive performance for preeclampsia than sFlt-1 or PlGF alone. However, the predictive value of the latter two cannot be ignored. Funding: Xinjiang Uygur Autonomous Region “Tianshan Innovation Team Plan”(2020D14010) Keywords: gestational hypertension; hypertension; meta-analysis; preeclampsia; predictive; PlGF; sFlt-1; sFlt-1/ PlGF, systematic review

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Diagnostic capacity of sFlt-1/PlGF ratio in fetal growth restriction: A systematic review and meta-analysis

et al. 2022

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Serum sFlt-1/PlGF ratio has better diagnostic ability in early- compared to late-onset pre-eclampsia

Cited by 6 publications

References 14 publications

Lower S-adenosylmethionine levels and DNA hypomethylation of placental growth factor (PlGF) in placental tissue of early-onset preeclampsia-complicated pregnancies

Lower S-adenosylmethionine levels and DNA hypomethylation of placental growth factor (PlGF) in placental tissue of early-onset preeclampsia-complicated pregnancies

Predictive Performance of sFlt-1, PlGF and the sFlt-1/PlGF Ratio for Preeclampsia: A Systematic Review and Meta-Analysis

Diagnostic capacity of sFlt-1/PlGF ratio in fetal growth restriction: A systematic review and meta-analysis

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