Serum Soluble Adhesion Molecules (sICAM-1, sVCAM-1 and sE-Selectin) in Healthy School Aged Children and Adults

Abstract

The aim of this study was to map normal levels of serum soluble isoforms of adhesion molecules in relation to age and sex in the group of school-aged children. sICAM-1, sVCAM-1 and sE-selectin were determined in the group of 158 normal children subdivided into two subgroups; 6-10 years (68 children, median age 8 years) and 11-15 years (90 children, median age 12 years) and in 70 normal adult blood donors (25 females and 45 males, median age 46 years). The levels of sICAM-1 and sE-selectin fell down significantly over the age range 6-15 years, while the level of sVCAM-1 was remained. Age-related normal ranges were established using correlation analysis and were expressed as the 5%-95% percentiles intervals: sICAM-1 206.8-486.8 ng/ml, sE-selectin 36.7-153.2 ng/ml in the group of 6-10 years old children, sICAM-1 184.1-354.0 ng/ml, sE-selectin 29.9-114.1 ng/ml in group of 11-15 years old children. The levels of sVCAM-1 were 359.6-822.0 ng/ml and were constant within the examined age interval from 6 to 15 years. The influence of sex was also assayed and it was not statistically significant in any age category tested. Normal ranges of sICAM-1 (60.2-218.4 ng/ml), sE-selectin (8.3-116.9 ng/ml) and sVCAM-1 (338.0-1148.0 ng/ml) were established for adult population of healthy blood donors using the same methods.

Assay validation and clinical performance of chronic inflammatory and chemokine biomarkers of NASH fibrosis

“…Further, the study authors did replicate and validate their results in a group of outpatient subjects from a hepatology clinic, a population more closely reflecting the participants in our study. Typically serum VCAM-1 ranges are approximately 340–1150 ng/mL in healthy adults [32], and we observed ranges from 391–1669 ng/mL in NAFLD/NASH patients. However, there is an order of magnitude difference between our study values and the Lefere results, which may be related to the measurement process, stressing the need for assay validation.…”

Immunologic Changes in TNF‐alpha, sE‐selectin, sP‐selectin, sICAM‐1, and IL‐8 in Pediatric Patients Treated for Psoriasis with the Goeckerman Regimen

“…TNF‐alpha stimulates the expression of adhesion molecules (E‐selectin, P‐selectin) and intercellular adhesion molecule 1 (ICAM‐1) (33) on both endothelial and immune cells. Skin biopsy specimens taken from inflamed skin of patients with psoriasis reveal up‐regulation of endothelial cell expression of P‐selectin, E‐selectin, and ICAM‐1 (34).…”

“…Skin biopsy specimens taken from inflamed skin of patients with psoriasis reveal up-regulation of endothelial cell expression of P-selectin, E-selectin, and ICAM-1 (34). Soluble forms of these molecules are detectable in serum and reflect the severity of the inflammatory reaction in the skin (33). Many studies focused on endothelial cell activity have revealed increased serum levels of soluble E-selectin in psoriatic patients, compared with healthy controls (14,35).…”

“…ICAM-1 presents an immunoglobulin-like adhesion molecule expressed on the surface of numerous cell types, including endothelial cells and cells involved in the immune response. It plays an important role in the adhesion and migration of leukocytes to the site of inflammation (33). ICAM-1 is expressed as either a membrane integral molecule or in a soluble form (33).…”

“…It plays an important role in the adhesion and migration of leukocytes to the site of inflammation (33). ICAM‐1 is expressed as either a membrane integral molecule or in a soluble form (33). The levels of sICAM‐1 are increased in many diseases, including psoriasis (36–38).…”