Serum Soluble ST2 Correlated with Symptom Severity and Clinical Response of Sublingual Immunotherapy for House Dust Mite-Induced Allergic Rhinitis Patients

Zhu, Kangshun; Cui, Xiuhong; Chen, Jingguo; Yu, Chao; Gao, Tianxi; Yan, Jing; Shao, Na; Zhu, Ping; Sun, Bin; Ren, Xiaoyong; Zhang, Yanni

doi:10.1155/2021/5576596

Cited by 8 publications

(12 citation statements)

References 39 publications

(64 reference statements)

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“…Fasting blood (5 mL) was collected from each participant before the onset of SLIT, and processed and stored as described previously. 13 A human cytokine standard 31-Plex assay kit (Bio-Rad) was utilized to detect 31 cytokines — BAFF, CCL2, CCL3, CCL4, CCL7, CCL11, CXCL9, CXCL10, CXCL12, GM-CSF, IFNγ, IL1α, IL1β, IL2, IL2Rα, IL4, IL5, IL6, IL7, IL8, IL9, IL10, IL13, IL15, IL16, IL17, IL33, M-CSF, MIF, TNFα, and TNFβ — based on the Luminex 200 system, and data interpretation was conducted as previously described. 19 All samples were diluted at 1:3 with sample diluent, and data from the 31-Plex assays were transferred to an Excel spreadsheet for further analysis.…”

Section: Methodsmentioning

confidence: 99%

“…During the SLIT, compliance education and follow-up were performed, medication score and total nasal symptom score (TNSS) evaluated via WeChat in the previous week and recorded weekly, and symptom and medication score (SMS) calculated as (TNSS + MS)/7, as previously described. 13 At 1-year follow-up, early efficacy of SLIT was evaluated based on change in SMS: patients who attained a 30% reduction in SMS from baseline were defined as responders and the rest nonresponders as per our previous study. 13 …”

Section: Methodsmentioning

confidence: 99%

“… 13 At 1-year follow-up, early efficacy of SLIT was evaluated based on change in SMS: patients who attained a 30% reduction in SMS from baseline were defined as responders and the rest nonresponders as per our previous study. 13 …”

Section: Methodsmentioning

confidence: 99%

“…[8][9][10] Conventional AIT can be performed subcutaneously or sublingually, and sublingual immunotherapy (SLIT) seems to be more popular because of its convenience, equivalent efficacy, and good tolerability. [11][12][13] Despite its widely accepted safety and effectiveness in AR patients, many users still respond poorly, and its effectiveness fluctuates widely. 9 Therefore, exploring biomarkers or methods for early identification of responders, monitor therapeutic effects in AR patients who have been assigned to SLIT is a research focus, and extremely pivotal for developing a precision medicine model and reducing the waste of medical resources.…”

Section: Introductionmentioning

confidence: 99%

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Multiple-Cytokine Profiling: A Novel Method for Early Prediction of the Efficacy of Sublingual Immunotherapy in Allergic Rhinitis Patients

Zhang¹,

Zhu²,

Cui³

et al. 2022

JIR

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Background Allergic rhinitis (AR) is a common inflammatory airway disease, and allergen-specific immunotherapy (AIT) is the only disease-modifying treatment for it. However, not all AR patients respond to AIT, and early prediction of patient response is extremely important. This study aimed to example serum levels of multiple cytokines in AR and explore their association with the efficacy of AIT. Methods A total of 74 AR patients treated with sublingual immunotherapy (SLIT) were prospectively recruited. Serum samples were obtained before the onset of SLIT and cytokine levels detected by multiplex analysis. All patients were followed for >1 year, and associations between cytokine levels and the early efficacy of SLIT were evaluated. Significantly distinctive cytokines were further verified in another independent cohort. Results Sixty patients completed the visit schedule set: 35 patients were put into a responder group and 25 a nonresponder group. Multiple-cytokine profiling showed that cytokine levels differed significantly between the two groups. The responder group had higher concentrations of BAFF and CCL11 and lower levels of CCL2, CCL7, IFNγ, IL8, IL10, IL16, and IL33 than the nonresponder group ( P <0.05). Receiver-operating characteristic curves highlighted that serum BAFF, IFNγ, IL10, and IL33 levels were strongly predictive of the efficacy of SLIT (area under the curve <0.7, P <0.05). Serum IL10 and IL33 were overexpressed in nonresponders in the validation cohort. Patients in the responder group exhibited significantly higher IL10 levels and lower IL33 post-SLIT than pre-SLIT ( P <0.05), but no statistical difference was found in nonresponders ( P <0.05). Conclusion Our data indicated that serum multiple-cytokine profiling was associated with response to SLIT and that IL10 and IL33 might serve as novel biomarkers for early prediction of efficacy and be involved in the therapeutic mechanisms of SLIT in AR patients.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiple-Cytokine Profiling: A Novel Method for Early Prediction of the Efficacy of Sublingual Immunotherapy in Allergic Rhinitis Patients

Zhang¹,

Zhu²,

Cui³

et al. 2022

JIR

Self Cite

View full text Add to dashboard Cite

show abstract

“…Prior publications demonstrated that AR was a heterogeneous clinical disease with a broad spectrum of severity, and limited indicators or biomarkers have been presented to monitor the disease progression and disease severity [ 5 ]. Evaluating disease severity utilizing biomarkers contributed to understand the underlying mechanisms of AR and monitor the therapeutic effect [ 6 , 7 ]. Therefore, exploring an accurate and reliable biomarker to assess AR severity is essential.…”

Section: Introductionmentioning

confidence: 99%

Circulating MMP-12 as Potential Biomarker in Evaluating Disease Severity and Efficacy of Sublingual Immunotherapy in Allergic Rhinitis

Zhou

Gǒng

et al. 2022

Mediators of Inflammation

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Background. Allergic rhinitis (AR) is a highly heterogeneous disease, and allergen-specific immunotherapy (AIT) is an effective treatment. This study aims to evaluate the circulating mas-related G protein-coupled receptor-X2 (MRGPRX2) and matrix metalloproteinase-12 (MMP-12) levels in evaluating disease severity and predicting efficacy of SLIT in AR patients. Methods. We enrolled 110 moderate-severe persist AR patients (AR group) and 40 healthy controls (HC group). Circulating levels of MRGPRX2 and MMP-12 were measured, and their associations with disease severity were evaluated. All AR patients were assigned to receive sublingual immunotherapy (SLIT), and the efficacy was evaluated, and serum samples were collected at 1 year and 3 years after treatment. The correlations between serum MRGPRX2 and MMP-12 and clinical efficacy were assessed. Results. The serum concentrations of MRGPRX2 and MMP-12 were significantly higher in the AR group than the HC group, and the elevated MMP-12 levels were correlated with VAS and TNSS, and serum MRGPRX2 levels were correlated with VAS. Finally, 100 and 80 patients completed 1-year and 3-year follow-up and were classified into effective and ineffective groups. Serum MRGPRX2 and MMP-12 levels were lower in the effective group than the ineffective group. Although serum MRGPRX2 and MMP-12 levels did not significantly change after 1 year SLIT, serum MMP-12 levels were decreased 3 years post-SLIT than baseline and 1 year post-SLIT levels. Receiver operating characteristic (ROC) showed that serum MMP-12 was a potential biomarker for predicting the efficacy of SLIT. Conclusion. Serum MRGPRX2 and MMP-12 appeared to be promising biological indicators in reflecting disease severity in AR patients. Moreover, circulating MMP-12 might serve as a reliable predictor for clinical responsiveness of SLIT.

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Association between IL1RL1 gene polymorphisms and allergic rhinitis risk in the Chinese Han population

Ren

Zhang

et al. 2022

Clinical Laboratory Analysis

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Background Although it has been confirmed that IL1RL1 is involved in the occurrence of allergic rhinitis (AR), the role of IL1RL1 gene single nucleotide polymorphisms (SNPs) in AR is still unclear. Methods We performed a case–control study including 1000 AR patients and 1000 healthy controls. The four SNPs rs72823628 G > A, rs950881 G > T, rs72823641 T > A and rs3771175 T > A in IL1RL1 were chosen and genotyped using Agena MassARRAY platform. The relationship between IL1RL1 SNPs and AR risk was analyzed by logistic regression and assessed with odds ratios (ORs) and corresponding 95% confidence intervals (95% CIs). Results Overall analysis revealed that IL1RL1 gene rs72823628, rs950881 and rs3771175 were associated with a reduced AR risk. Stratified analysis showed that the three SNPs (rs72823628, rs950881 and rs3771175) were obviously linked to a reduced risk of AR in males. Moreover, no correlation was observed between haplotypes and reduced AR risk after the false discovery rate (FDR) correction. The false positive report probability (FPRP) analysis was used to further validate significant findings. Conclusion Our study is the first to indicate that IL1RL1 gene polymorphisms (rs72823628, rs950881 and rs3771175) may be correlated with decreased risk of AR in the Chinese Han population.

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Serum Soluble ST2 Correlated with Symptom Severity and Clinical Response of Sublingual Immunotherapy for House Dust Mite-Induced Allergic Rhinitis Patients

Cited by 8 publications

References 39 publications

Multiple-Cytokine Profiling: A Novel Method for Early Prediction of the Efficacy of Sublingual Immunotherapy in Allergic Rhinitis Patients

Multiple-Cytokine Profiling: A Novel Method for Early Prediction of the Efficacy of Sublingual Immunotherapy in Allergic Rhinitis Patients

Circulating MMP-12 as Potential Biomarker in Evaluating Disease Severity and Efficacy of Sublingual Immunotherapy in Allergic Rhinitis

Association between IL1RL1 gene polymorphisms and allergic rhinitis risk in the Chinese Han population

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