Serum steroid profiling of hepatocellular carcinoma associated with hyperadrenocorticism in dogs: A preliminary study

Abstract

BackgroundHepatocellular carcinoma (HCC) is one of the most common primary liver tumors in humans and dogs. Excessive adrenocortical hormone exposure may cause steroid hepatopathy, which may develop into HCC. In our previous study, hyperadrenocorticism (HAC) was a highly concurrent disease in dogs with HCC. Therefore, this study hypothesized that adrenal steroid alterations might be involved in hepatocarcinogenesis and aimed to specify the relationship between HAC and HCC in dogs.Materials and methodsThis study included 46 dogs brought to the Hokkaido University Veterinary Teaching Hospital between March 2019 and December 2020. Owners gave their signed consent for blood collection on their first visit. A total of 19 steroids (14 steroids and 5 metabolites) in the baseline serum of 15 dogs with HCC, 15 dogs with HAC, and 10 dogs with both diseases were quantitatively measured using the developed liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method.ResultsIn each group, 11 steroids were detected higher than 50%. The detection rate of steroid hormones did not significantly differ between the groups (p > 0.05). Principle component analysis (PCA) showed that the steroid profiles of the three groups were comparable. Median steroid hormone concentrations were not significantly different between the study diseases (p > 0.05).ConclusionThe developed LC/MS/MS was useful for measuring steroid hormones. Although it was clear that HAC was concurrent in dogs with HCC, none of the serum steroids was suggested to be involved in HCC.

Neurotoxicity and behavioral disorders induced in mice by acute exposure to the diamide insecticide chlorantraniliprole

“…The analytical method for steroid hormones was according to our previously reported method [ 40 ]. In brief, the 25 µL of plasma was thoroughly mixed with 10 µL of IS mix and 90 µL of acetonitrile containing 1% formic acid, and the mixture was centrifuged (10,000 × g , 10 min, 25°C).…”

“…Then, 100 µL of 50% methanol-double distilled water containing 0.1% formic acid was added, and the target substances were measured by LC-ESI/MS/MS. An Agilent 1260 Infinity II HPLC system (Agilent Technologies) was used for LC, and an Agilent 6495B triple quadrupole mass spectrometer (Agilent Technologies) was used for MS as described elsewhere [ 40 ].…”

“…They are attracting attention as alternatives to organophosphorus and neonicotinoid pesticides, which have been disputed as toxic. However, CAP has been reported to cause genotoxic, behavioral, and endocrine effects in aquatic organisms and vertebrates [ 9 , 28 , 34 , 37 , 40 , 48 , 61 ], and the binding site of CAP has been identified in mammalian RyR1 [ 32 ].…”

Novel Y RNA-Derived Fragments Can Differentiate Canine Hepatocellular Carcinoma from Hepatocellular Adenoma

“…Hepatocellular adenoma (HCA) and hepatocellular carcinoma (HCC) can occur in both canine and human patients [1]. HCAs are rare, benign tumors that derive from proliferating hepatocytes, whereas HCCs are common, malignant tumors that can develop from HCAs [2].…”