Serum testing of genetically modified soybeans with special emphasis on potential allergenicity of the heterologous protein CP4 EPSPS

Abstract

Serum testing of genetically modified soybeans with special emphasis on potential allergenicity of the heterologous protein CP4 EPSPS" (2007). Faculty Publications in Food Science and Technology. 156.

Soy allergy in perspective

“…CP4 EPSPS protein did not bind significant quantities of IgE in soy-sensitive patients and there was no evidence for an increased allergenic potential of this biotech protein [41 ]. IgE binding pattern to extracts from genetically modified and wild type soybeans were found to be identical in western blotting experiments.…”

“…In a recent study, Hoff et al [41 ] determined the potential for developing allergic sensitization to the CP4 EPSPS protein in two different patient populations: children with atopic dermatitis and adult individuals with documented soy allergy and control groups. CP4 EPSPS protein did not bind significant quantities of IgE in soy-sensitive patients and there was no evidence for an increased allergenic potential of this biotech protein [41 ].…”

Characterization of Cannabis Sativa Allergens

“…Recently, several reports have suggested that RuBisCO can be an allergen in other plants. [36][37][38] Another IgE binding protein identified in this study was ATP synthase. In one study, sequence homology was demonstrated between a bovine allergen and one of the subunits of ATP synthase which forms the basis for a potential cross-reactivity.…”

Allergenicity Assessment of a Genetically Modified Protein-Recombinant Human Lactoferrin

“…Immunoblots were conducted according to the previous protocol [49][50][51] with some modifications. In brief, the SDS-PAGE was conducted using 400 ng purified rhLF, bLF, hLF (kindly provided by professor Li Ning, Purity: 98%) and 10 μg protein extract prepared from a standard egg powder (Lot.…”