Serum testosterone concentration in surgically castrated Black Bengal goats

Hasan, Moinul; Miah, M.A.H.; Rosy, TA; Jha, Pradeep Kumar; Juyena, Nasrin Sultana

doi:10.3329/bvet.v33i2.36460

Cited by 8 publications

(7 citation statements)

References 14 publications

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“…The resolution of the post castration inflammation and associated swelling indicates healing of damaged tissue and cessation of spermatogenesis provided that the castration was successful. Testosterone production is usually affected as was previously reported that testosterone concentration was significantly decreased from 6.1 ng/ml on the day of castration to 0.6 ng/ml 30 days later (Al-Asadi and Al-Kadi 2012; Hasan et al 2016). As was observed for the testes, the diameters of the left and right spermatic cords also increased after castration with Burdizzo, CaCl2 and Olive oil but were decreased by day 28 after castration.…”

Section: Discussionmentioning

confidence: 54%

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Effectiveness of Castration with Burdizzo, Calcium Chloride and Olive Oil in ‎Sahel Bucks

Laku

Mohammed

Bukar

2021

SJVS

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We evaluated the efficacy of castration with bilateral intratesticular injections of Calcium Chloride Dihydrate (CaCl2) in ethanol, Olive Oil as well as Burdizzo technique in twenty Sahel bucks. The bucks were randomly distributed into four groups as A, B, C and D. Group A served as the control and were given bilateral intratesticular injection of 1.0 ml of normal saline. Bucks in group B were castrated with Burdizzo, while group C and D were given bilateral intra testicular injection of 1.0 ml of CaCl2 and Olive oil, respectively. The sonograms and semen profiles of the bucks were evaluated.The diameters of the testicles and spermatic cords and the semen parameters were measured in real time after castration at days 1, 7, 14, 21, 28 and 60. The diameters in the Burdizzo, CaCl2‎and Olive oil castrated bucks ‎significantly increased (P < 0.05) on days 7 and was decreased at ‎day 60.‎ Semen motility was absent in the bucks castrated with Cacl2 after day 14. The ‎concentration of spermatozoa also decreased significantly on day 7 in all the Burdizzo, CaCl2‎and ‎Olive oil castrated goats. Sonogram of testes castrated with Cacl2 showed discreet focal ‎hyperechoic, surrounded by ‎hypoechoic areas ‎within the parenchyma due to the CaCl2 deposition. The testes of bucks castrated with olive oil were ‎characterized by a central hypoechoic area ‎surrounded by clearly distinguishable hyperechoic ‎areas within the parenchyma. It was concluded that intratesticular injection with Burdizzo, CaCl2and olive oil resulted in successful castration but CaCl2 injection resulted in earlier azoospermia.

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Section: Discussionmentioning

confidence: 54%

“…Castration involves the extirpation or suppression of gonadal function (Neto et al, 2014). Following orchiectomy in goats, serum testosterone concentration significantly (P < 0.01) decreased from 6.1 ± 0.2 ng/mL on the day of castration to 0.6 ± 0.0 ng/mL 30 days later (Hasan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Castration with Burdizzo, Calcium Chloride and Olive Oil in ‎Sahel Bucks

Laku

Mohammed

Bukar

2021

SJVS

View full text Add to dashboard Cite

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“…After collection, the blood was centrifuged at 3000 rpm for 20 minutes to collect serum. Serum was collected in Eppendorf tubes and preserved at -20° C (Talukder et al, 2018;Hasan et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

“…Funding for this work was provided by the Ministry of Science and Technology, Government of the People's Republic of Bangladesh through National Science and Technology Fellowship/2015-2016 (Grant No. 93.012.002.01.03.022-2015…”

Section: Acknowledgmentsmentioning

confidence: 99%

Clinical evaluation of chemo-sterilization through histomorphology and hormonal changes in bucks

Hasan¹,

Miah²,

Alam³

et al. 2021

SA J. An. Sci.

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The study was aimed to evaluate methods for nonsurgical castration of Black Bengal bucks by intra-testicular injection of calcium chloride, sodium chloride, citric acid solutions or sterile deionized water. Twelve healthy bucks were randomly allotted to groups A, B, C, and D, consisting of three bucks each. The local anesthetic, 2% lidocaine hydrochloride, was infused into the spermatic cord of each buck, followed by bilateral intra-testicular injections of 30% calcium chloride (CaCl2), 25% sodium chloride (NaCl), 50% citric acid (C6H8O7) solutions, and sterile deionized water dosed at 2 ml per testis in groups A, B, C and D respectively. To evaluate the efficacy of chemical agents on the inactivation of testes, clinical parameters, changes in scrotal circumference, testicular fine needle aspiration (TFNA), histopathology and serum concentration of testosterone and LH were monitored. A significant decrease in the scrotal circumference was observed between the intra-testicular injection and day 14 in all the bucks. Absence of spermatogenic cells and spermatozoa in the testicular biopsy was observed on day 14 post injection in the bucks, except for one in group C. Histopathology revealed massive destruction of seminiferous tubules and disorganization of the testicular parenchyma. Serum testosterone concentration declined significantly on day 14 compared with day 0. Consequently, the gradual elevation in serum LH concentration was significant. Thus, intra-testicular injections of CaCl2 and NaCl were more effective than C6H8O7 in inducing chemical-based nonsurgical castration.

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“…A 3 mL blood sample was collected from the jugular vein daily with appropriate asceptic measures, without using any anticoagulant prior to ultrasonography. Then, serum was collected after centrifuging at 1,500 rpm for 15 min and stored at −80°C until determination by ELISA of the concentrations of progesterone and oestradiol hormone (17). The serum sample was equilibrated at room temperature for half an hour.…”

Section: Methodsmentioning

confidence: 99%

Monitoring vaginal electrical resistance, follicular waves, and hormonal profile during oestrous cycle in the transition period in Bangladeshi sheep

Talukder

Hasan

Rosy

et al. 2018

Journal of Veterinary Research

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IntroductionThe ovarian follicular dynamics, vaginal electrical resistance (VER), progesterone (P4) and oestrogen (E2) profiles were investigated during the oestrous cycle in four indigenous ewes.Material and MethodsDaily VER values were recorded with a heat detector. The follicles were observed and measured by trans-rectal ultrasonography. Blood was collected daily for hormonal profiles.ResultsA significant variation in VER values (P < 0.05) in oestrus by ewes and position in the sequence of cycles was observed. Trans-rectal ultrasonography of ovaries revealed the presence of 2–4 waves of follicular growth. Study of hormonal profiles by ELISA revealed a positive correlation between E2 concentration and development of follicles and a negative correlation between P4 concentration and their development. The concentrations of oestradiol increased in oestrus and then decreased to a basal level. Follicular growth was accompanied by a rise in the concentration of serum oestradiol. Inversely, when follicles received the stimulation for ovulation, concentration of progesterone started to fall, but after ovulation, it climbed back to its peak and remained at this state until next ovulatory follicle reached its maximum diameter.ConclusionThis study could help to set up a manipulative reproductive technique for improving genetic values in indigenous sheep.

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Serum testosterone concentration in surgically castrated Black Bengal goats

Cited by 8 publications

References 14 publications

Effectiveness of Castration with Burdizzo, Calcium Chloride and Olive Oil in ‎Sahel Bucks

Effectiveness of Castration with Burdizzo, Calcium Chloride and Olive Oil in ‎Sahel Bucks

Clinical evaluation of chemo-sterilization through histomorphology and hormonal changes in bucks

Monitoring vaginal electrical resistance, follicular waves, and hormonal profile during oestrous cycle in the transition period in Bangladeshi sheep

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