Serum tryptophan and its metabolites in female dogs undergoing ovariohysterectomy as treatment of pyometra or as elective spay surgery

Dąbrowski, Roman; Hagman, Ragnvi; Tvarijonaviciute, Asta; Pastor, Josep; Kocki, Tomasz; Turski, Waldemar A.

doi:10.1016/j.theriogenology.2015.01.009

Cited by 11 publications

(6 citation statements)

References 44 publications

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“…Bitches of both groups had participated in an earlier study in which we studied insulin-like growth factor I, C-reactive protein, and tryptophan [ 10 , 11 ]. All experimental protocols and procedures were approved by the First Local Ethics Commission for Animal Experiments in Lublin, Poland.…”

Section: Resultsmentioning

confidence: 99%

Serum IL-6 and IL-10 concentrations in bitches with pyometra undergoing ovariohysterectomy

et al. 2015

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BackgroundPyometra is a serious bacterial infection of the uterus affecting female dogs and manifests as an accumulation of pus in the uterine lumen. The aim of the study was to assess changes in serum interleukin (IL)-6 and IL-10 concentrations in bitches with pyometra undergoing ovariohysterectomy.FindingsBlood samples were collected from healthy bitches (controls) and bitches with pyometra before surgery, and 3 and 10 days after ovariohysterectomy. Before surgery, bitches with pyometra had significantly higher serum concentrations of IL-6 and IL-10 than the controls. After surgery, the serum concentration of IL-6 and IL-10 decreased significantly. In healthy dogs, the concentration of IL-6 and IL-10 showed a significant increase 3 days after surgery followed by a decrease on day 10.ConclusionAn increase in serum concentrations of IL-6 and IL-10 was present before surgery in bitches with pyometra and 3 days after ovariohysterectomy in healthy controls. Concentrations decreased after ovariohysterectomy and/or proper healing, suggesting that these cytokines can be useful for assessment of the postoperative period in bitches.

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Section: Resultsmentioning

confidence: 99%

Serum IL-6 and IL-10 concentrations in bitches with pyometra undergoing ovariohysterectomy

et al. 2015

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“…25 A study reported a mean and standard deviation (SD) of serum tryptophan concentrations using high performance liquid chromatography from samples that had been frozen for a shorter duration (5 months) in 9 clinically healthy bitches of 5 different breeds as 68.44 and 21.77 nmol/mL, which was not dissimilar to the results obtained in our apparently healthy group, using frozen serum stored for a longer duration. 26 However, further studies are needed to determine the effect of prolonged storage in…”

Section: Discussionmentioning

confidence: 99%

Alterations in serum amino acid concentrations in dogs with protein‐losing enteropathy

Kathrani

Allenspach

Fascetti

et al. 2018

Veterinary Internal Medicne

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BackgroundCertain amino acids are decreased in humans with inflammatory bowel disease (IBD) and supplementation with the same amino acids has shown beneficial effects in animal models of IBD. Currently, the amino acid status of dogs with protein‐losing enteropathy (PLE) is unknown.Hypothesis/ObjectiveTo determine if serum amino acid concentrations are abnormal in dogs with PLE and correlated with clinical and laboratory variables and outcome.AnimalsThirty client‐owned dogs diagnosed with PLE and 12 apparently healthy dogs seen at Bristol Veterinary School.MethodsRetrospective study using stored residual serum from fasted dogs with PLE, collected at the time of diagnostic investigation and from apparently healthy dogs. Serum was analyzed for 30 amino acids using an automated high‐performance liquid chromatography amino acid analyzer.ResultsSerum tryptophan concentrations were significantly decreased in dogs with PLE (median, 22 nmol/mL; range, 1–80 nmol/mL) compared with apparently healthy control dogs (median, 77.5 nmol/mL; range, 42–135 nmol/mL, P < .001). There were no significant differences in the remaining 29 serum amino acids between dogs with PLE and apparently healthy. Serum tryptophan concentrations were also significantly correlated with serum albumin concentrations in dogs with PLE (P = .001, R 2 = 0.506).Conclusions and Clinical ImportanceDecreased serum tryptophan concentration might play a role in the pathogenesis of canine PLE or be a consequence of the disease.

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“…After prolonged HPA stimulation, as in chronic pain and stress disorders, cortisol has been shown to decrease in both humans and animals [ 45 , 48 , 51 , 52 ]. It can therefore not be excluded that the decreased cortisol and CST levels seen at recall might indicate an underlying persistent stress response after the OHE procedure [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Catestatin, vasostatin, cortisol, temperature, heart rate, respiratory rate, scores of the short form of the Glasgow composite measure pain scale and visual analog scale for stress and pain behavior in dogs before and after ovariohysterectomy

et al. 2016

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BackgroundThe stress reaction induced by surgery and associated pain may be detrimental for patient recovery and should be minimized. The neuropeptide chromogranin A (CGA) has shown promise as a sensitive biomarker for stress in humans. Little is known about CGA and its derived peptides, catestatin (CST) and vasostatin (VS), in dogs undergoing surgery. The objectives of this study were to investigate and compare concentrations of CGA epitopes CST and VS, cortisol, body temperature, heart rate, respiratory rate, scores of the short form of the Glasgow composite measure pain scale (CMPS-SF) and visual analog scales (VAS) for stress and pain behavior in dogs before and after ovariohysterectomy.MethodsThirty healthy privately owned female dogs admitted for elective ovariohysterectomy were included. Physical examination, CMPS-SF, pain behavior VAS, and stress behavior VAS were recorded and saliva and blood samples were collected before surgery, 3 h after extubation, and once at recall 7–15 days after surgery. Dogs were premedicated with morphine and received carprofen as analgesia for 7 days during the postoperative period.ResultsAt 3 h after extubation, CMPS-SF and pain behavior VAS scores had increased (p < 0.0001) and stress behavior VAS scores, temperature, respiratory rate (p < 0.0001), plasma CST concentrations (p = 0.002) had decreased significantly compared to before surgery. No significant differences were observed in the subjective and physiological parameters between before surgery and at recall, but plasma CST (p = 0.04) and serum cortisol (p = 0.009) were significantly lower at recall. Plasma VS, saliva CST, and heart rate did not differ significantly at any observed time.ConclusionStudy parameters for evaluating surgery-induced stress and pain changed in dogs subjected to ovariohysterectomy. To further evaluate CST and VS usefulness as pain biomarkers, studies on dogs in acute painful situations are warranted.

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Serum tryptophan and its metabolites in female dogs undergoing ovariohysterectomy as treatment of pyometra or as elective spay surgery

Cited by 11 publications

References 44 publications

Serum IL-6 and IL-10 concentrations in bitches with pyometra undergoing ovariohysterectomy

Serum IL-6 and IL-10 concentrations in bitches with pyometra undergoing ovariohysterectomy

Alterations in serum amino acid concentrations in dogs with protein‐losing enteropathy

Catestatin, vasostatin, cortisol, temperature, heart rate, respiratory rate, scores of the short form of the Glasgow composite measure pain scale and visual analog scale for stress and pain behavior in dogs before and after ovariohysterectomy

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