Serum vitamin D concentrations in hospitalized critically ill dogs

Jaffey, Jared A.; Backus, Robert C.; McDaniel, Kaylyn M.; DeClue, Amy E.

doi:10.1371/journal.pone.0194062

Cited by 28 publications

(38 citation statements)

References 51 publications

(52 reference statements)

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“…Kim et al (2017) observed that dogs that survived to acute pancreatitis had concentrations of 25(OH)D higher than concentrations observed in dogs that died due to this disease before the beginning of treatment. Jaffey, Backus, McDaniel, and DeClue (2018) found that dogs critically ill or with sepsis had lower serum 25(OH)D concentrations compared to healthy control dogs. Besides, serum 25(OH)D was considered a good predictor of survival for dogs in the intensive care unit and 30 days after discharge and it presented a correlation with illness severity.…”

Section: Vitamin D As a Predictor Of Mortalitymentioning

confidence: 89%

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Vitamin D metabolism in dogs and cats and its relation to diseases not associated with bone metabolism

Zafalon

Risolia

Pedrinelli

et al. 2019

Animal Physiology Nutrition

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Due to the presence of receptors in the cells of numerous body tissues, vitamin D is associated with several physiological functions that go beyond calcium and phosphorus homoeostasis and control of bone metabolism in the body. In humans, several studies have associated lower vitamin D concentrations with numerous diseases, such as cancer, heart disease, autoimmune diseases and infectious diseases, and also with an increase in the total mortality rate of the population. Recently, this nutrient started to gain importance in veterinary medicine, and several articles have shown a correlation between low vitamin D status and diseases unrelated to bone metabolism. The present review aims to highlight the recent publications that investigated this relationship, bringing the evidence that exists so far in dogs and cats. K E Y W O R D S1,25(OH)2D, 25(OH)D, calcidiol, calcitriol, cholecalciferol, hypovitaminosis D

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Section: Vitamin D As a Predictor Of Mortalitymentioning

confidence: 89%

“…(Melamed et al, 2008). Jaffey, Backus, McDaniel, and DeClue (2018) found that dogs critically ill or with sepsis had lower serum 25(OH)D concentrations compared to healthy control dogs. was not considered a predictor of mortality as there was not a linear regression .…”

Section: In Humans Vitamin D Supplementation In Chf Patients Has Beenmentioning

confidence: 99%

Vitamin D metabolism in dogs and cats and its relation to diseases not associated with bone metabolism

Zafalon

Risolia

Pedrinelli

et al. 2019

Animal Physiology Nutrition

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“…Another limitation is that we enrolled only apparently healthy dogs. Calcitriol could have a different effect on shelter dogs that were systemically ill because they would be expected to have decreased serum 25(OH) D concentrations compared to healthy dogs [20]. Humans that are vitamin D deficient experience the most clinical benefit when supplemented with oral vitamin D [22].…”

Section: Discussionmentioning

confidence: 99%

Effects of calcitriol on oxidative burst, phagocytic function, and leukocyte cytokine production in shelter dogs

Jaffey

Bessette

Tao

et al. 2020

Canine Genet Epidemiol

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Background: The active metabolite of vitamin D, calcitriol, has been shown across many different species to augment innate immune responses and dampen aberrant proinflammatory cytokine production. Community acquired infections are common in shelters and consume limited shelter resources, impact adoption rates, and can result in unnecessary euthanasia. Prophylactic oral vitamin D supplementation decreases the incidence and severity of upper and lower respiratory tract infections in humans. Before a clinical trial investigating the clinical benefit of oral vitamin D supplementation in shelter dogs can be pursued, an in vitro study evaluating the immunomodulatory effects of calcitriol in blood from shelter dogs is warranted. Therefore, the objective of this study was to determine if incubation of whole blood obtained from apparently healthy dogs housed in a shelter for ≥7 days with calcitriol would alter granulocyte/monocyte (GM) oxidative burst and phagocytic function as well as pathogen-associated molecular pattern (PAMP)-stimulated leukocyte production of tumor necrosis factor (TNF)-α, and interleukin (IL)-6, and IL-10. Results: Ten dogs housed in a shelter for ≥7 days were enrolled in a prospective cohort study. Whole blood from these dogs was incubated with calcitriol (10 − 7 M) or diluent (control) for 24 h. Subsequent to this incubation, phagocytosis of opsonized-Escherichia coli (E. coli) and E. coli-induced oxidative burst were evaluated via flow cytometry. In addition, leukocyte production of TNF-α, IL-6, and IL-10 were measured using a canine-specific multiplex bead assay. Calcitriol significantly decreased leukocyte TNF-α production (p = 0.009) and increased IL-10 production (p = 0.002). Tumor necrosis factor-α-toIL -10 ratio was significantly decreased with calcitriol (p = 0.017), while IL-6 production as well as GM oxidative burst and phagocytic function were not significantly affected. Conclusions: These data indicate that calcitriol attenuates proinflammatory immune responses without affecting GM oxidative burst or phagocytic function in vitro in whole blood obtained from apparently healthy shelter dogs.

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“…Hypovitaminosis D has been identi ed in many infectious diseases in dogs including leishmaniasis, babesiosis, spirocercosis, blastomycosis, and bacterial sepsis, which suggests that like people, vitamin D could have an important protective role in the canine innate immune response (16)(17)(18)(19)(20). This theory is corroborated by early in vitro studies in healthy and critically ill dogs that demonstrated incubation of blood with calcitriol, decreased leukocyte production of the pro-in ammatory cytokine tumor-necrosis factor (TNF)-α in a concentration dependent manner (11,21).…”

Section: Introductionmentioning

confidence: 99%

Effects of calcitriol on oxidative burst, phagocytic function, and leukocyte cytokine production in shelter dogs

Jaffey

Bessette

Tao

et al. 2020

Preprint

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Background The active metabolite of vitamin D, calcitriol, has been shown across many different species to augment innate immune responses and dampen aberrant proinflammatory cytokine production. Community acquired infections are common in shelters and consume limited shelter resources, impact adoption rates, and can result in unnecessary euthanasia. Prophylactic oral vitamin D supplementation decreases the incidence and severity of upper and lower respiratory tract infections in humans. Before a clinical trial investigating the clinical benefit of oral vitamin D supplementation in shelter dogs can be pursued, an in vitro study evaluating the immunomodulatory effects of calcitriol in blood from shelter dogs is warranted. Therefore, the objective of this study was to determine if incubation of whole blood obtained from apparently healthy dogs housed in a shelter for ≥ 7 days with calcitriol would alter granulocyte/monocyte (GM) oxidative burst and phagocytic function as well as pathogen-associated molecular pattern (PAMP)-stimulated leukocyte production of tumor necrosis factor (TNF)-α, and interleukin (IL)-6, and IL-10. Results Ten dogs housed in a shelter for ≥ 7 days were enrolled in a prospective cohort study. Whole blood from these dogs was incubated with calcitriol (10-7 M) or ethanol (control) for 24 h. Subsequent to this incubation phagocytosis of opsonized-Escherichia coli (E. coli) and E. coli-induced oxidative burst were evaluated via flow cytometry. In addition, leukocyte production of TNF-α, IL-6, and IL-10 were measured using a canine-specific multiplex assay. Calcitriol significantly decreased leukocyte TNF-α production (p = 0.009) and increased IL-10 production (p = 0.002). Tumor necrosis factor-α-to-IL-10 ratio was significantly decreased with calcitriol (p = 0.017), while IL-6 production as well as GM oxidative burst and phagocytic function were not significantly affected. Conclusions These data indicate that calcitriol attenuates proinflammatory immune responses without affecting GM oxidative burst or phagocytic function in vitro in whole blood obtained from apparently healthy shelter dogs.

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Serum vitamin D concentrations in hospitalized critically ill dogs

Cited by 28 publications

References 51 publications

Vitamin D metabolism in dogs and cats and its relation to diseases not associated with bone metabolism

Vitamin D metabolism in dogs and cats and its relation to diseases not associated with bone metabolism

Effects of calcitriol on oxidative burst, phagocytic function, and leukocyte cytokine production in shelter dogs

Effects of calcitriol on oxidative burst, phagocytic function, and leukocyte cytokine production in shelter dogs

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