Serum Vitamin D Is Associated with Antioxidant Potential in Peri-Parturient Cows

Strickland, Jaimie M.; Wisnieski, Lauren; Mavangira, Vengai; Sordillo, Lorraine M.

doi:10.3390/antiox10091420

Cited by 8 publications

(5 citation statements)

References 63 publications

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“…As well known, vitamin D 3 is the strong antioxidant which may inhibit LP in tissues. Vitamin D 3 protects cells membranes from destroying by free radicals, supports their antioxidant capacity (considerably decreases iNOS expression, ROS and malondialdehyde production) [17,18]. In this way vitamin D 3 inhibits LP and reduces OS.…”

Section: Results and Their Discussionmentioning

confidence: 99%

The Impact of Cholecalciferol on the Sex Hormones Level and Serum Biochemical Indices in Rat Males With Experimental Gonadopathy

Belkina

Velychko

Marakhovskyi

et al. 2023

PEP

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The testicular deficiency, cryptorchidism, varicocele, endocrine pathologies are the main causes of men infertility. The internal diseases of testicles lead to fertility declining in the major part of cases which explains the importance of studying of gonadopathies᾿ methods of correction. In recent times, vitamin D is noted among remedies suitable for effective correction of reproductive system. The aim of investigation: to determine the sex hormones levels and biochemical indices in serum of rats’ males with experimental testicles damage under the condition of vitamin D3 correction used separately or in combination with Tribestan®. Materials and methods. The experiment has been carried out on mature Vistar rats’ males; group of animals: S-model (animals with serotonin-induced gonads damage, gonadopathy (GP)); S+solvent (rats with gonadopathy which received solvent); S+vit. D3 (animals with gonadopathy received vitamin D3); S+Tr (rats with gonadopathy obtained Tribestan (Tr) – reference medicine); S+vit. D3+Tr (animals received both remedies); control – intact males. The activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), general content of nitrogen oxide cycle metabolites (NОх), concentration of TBARS and sex hormones Ts and E2 have been determined; their ratio has been calculated. Results. The NOх was increased in S-model rats. The application of vit. D3 or Tr has caused decreasing of NOх concentration in comparison with S-model group. The complex using of vit. D3 and Tr has had positive effect as for protection against oxidative stress (OS). The concentration of NOx in rats of S+vit. D3+Tr was by 56% less than in S-model group, but, as in the other experimental groups of animals, has remained increased relative to control group. The deficiency of free arginine in blood serum has been detected in all experimental groups comparing with Control group. The application of vit. D3 hasn’t affected on the arginine content. Tr has positively influenced the arginine level: the increasing of concentration of this amino acid was 95% relative to S-model group, but was less by 48% than values of intact animals. After application of Tr and vit. D3 together the concentration of arginine was higher by 2.2 times than in rats of S-model group, but hasn’t reached the values of intact animals. The concentration of TBARS has increased under the condition of GP. The using of vit. D3 has led to reducing, but not to normalizing of TBARS content. The concentration of TBARS in rats of S+Tr group was by 1.9 times lower than in rats with GP and by two times higher than in intact animals. Simultaneous using of Tr and vit. D3 has been effective as for reducing of lipid peroxidation (LP) intensity. The concentration of TBARS in S+vit. D3+Tr group has declined by 2.2 times comparing with values of animals with GP. The combination of Tr and vitamin D3 was more effective than cholecalciferol single using as for protection against OS and LP reducing and has demonstrated activity at the level of reference drug. GP has led to increasing of ALT activity 2.4 times and AST – by 2 times comparing with control group. The application of Tr, vit. D3 or their complex hasn’t changed ALT activity which was statistically higher than intact animals’ values. The activity of AST was increased in animals with GP and reduced after experimental correction of GP consequences. Vitamin D3 monotherapy has reduced by 32% the activity of AST comparing with animals with GP. AST concentration in males’ blood serum was decreased too with the combined use of Tr and vitamin D3 comparing with untreated animals. Thus, vitamin D has positively impacted the AST level in combined use with Tr as well as monotherapy. Serotonin-induced experimental GP has caused the declining of general Ts concentration in blood serum of animals of all experimental groups. The application of vitamin D3 hasn’t demonstrated androgen correcting activity. Tr has positively influenced androgen synthesized function: Ts concentration has increased by 95% comparing with S-model group, but remained lower by 31% than in intact animals. The Ts concentration in S+vit. D3+Tr group was 2.1 times higher than in animals with GP. However, Ts values in this group haven’t reached Control group values and were by 25% less. The concentration of estradiol in all experimental groups has corresponded to the values of intact animals. The calculation of Ts/Е2 ratio has shown to be decreased in S-model, S+solvent, S+vit. D3 groups in comparison with intact animals. The Ts/Е2 ratio has normalized in groups with GP treated by reference drug separately or in combination with vitamin D3. Conclusions: The application of cholecalciferol has considerably decreased NOx and TBARS concentrations, but hasn’t affected on arginine level which has declined with the gonadopathy. Vitamin D has caused reducing of aspartate aminotransferase activity in serum and hasn’t had androgenic and estrogenic effects. The application of Tribestan has led to the pro-oxidative/antioxidative balance recovery under the condition of experimental gonadopathy. This medicine has reduced the high level of aspartate aminotransferase activity, increased testosterone level and normalized testosterone/estradiol ratio in blood serum of experimental animals. The application of vitamin D and Tribestan in combination has led to improvement of androgenic status and has demonstrated better effect as for protection against oxidative stress and lipid peroxidation than cholecalciferol as a monotherapy.

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Section: Results and Their Discussionmentioning

confidence: 99%

The Impact of Cholecalciferol on the Sex Hormones Level and Serum Biochemical Indices in Rat Males With Experimental Gonadopathy

Belkina

Velychko

Marakhovskyi

et al. 2023

PEP

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“…Vitamin D also played an important role in calcium absorption (Eder and Grundmann, 2022). Calcium ion played a role in myometrial contractions in the third phase of labor (Strickland et al, 2021). Sunlight was useful for vitamin D synthesis in cow skin (Weir et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Managing retained placenta in first-parity doe and administering vitamin A, D, and E as supportive treatment

Dhea Salsabila,

Viski Fitri Hendrawan

2024

Ovz

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This study aims to report the treatment of retained placenta in a doe by administration of intrauterine antibiotics, accompanied by intramuscular injection of antibiotics, anti-inflammatory, and injection of vitamins A, D, and E as supportive treatment. The Saanen doe was brown, approximately two years old with a BCS of 3/5 and kidding for the first time on January 11, 2023. The following day, it was reported that the doe's placenta had not been expelled until 24 hours after kidding. Physically the doe was weak, unable to stand, and reddish-brown discharge was seen came out of the vulva. Based on these conditions the doe was diagnosed as having retained placenta with a fausta prognosis. The doe was treated with a bolus of antibiotics contained 250 mg sulphadiazine and 50 mg trimethoprim which was diluted with 5 mL of 0.9% NaCl for uterine lavage. Systemic treatment consisted of intramuscular injection of ceftiofur at 1.1 mg/kg bw, flunixin meglumine at 1.1 mg/kg bw, as well as a combination of 300,000 IU of vitamin A palmitate, 100,000 IU of vitamin D3, and 50 mg of vitamin E acetate as supportive treatment. The treatment was successful, the doe was able to stand and eat when examined the next day after treatment. The doe returned to estrus 42 days after treatment. It could be concluded that treatment of retained placenta in a doe with intrauterine broad-spectrum antibiotics, beta-lactamase antibiotics and intramuscular anti-inflammatory, with vitamins A, D and E as supportive therapy was effective and the doe returned to estrus 42 days after treatment.

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“…Furthermore, vitamin D treatment has been associated with increased cell viability and the inhibition of S. aureus adhesion and invasion in bovine mammary epithelial cells, underscoring its potential significance in mastitis prevention ( 202 ). Consistently, a recent published article has demonstrated that vitamin D and its metabolites hydroxyvitamin D [25(OH)D] with a concentration of 20-400 ng/ml has shown a positive role on bovine immune cells, antioxidant response and could be consider as therapeutic agent for mastitis prevention ( 203 – 205 ).In light of the available literature, it is evident that vitamin D holds a central position in bolstering dairy cattle immunity and potentially alleviating bovine mastitis. However, it is worth noting that further comprehensive investigations are warranted to gain a deeper understanding of its precise mechanisms and potential therapeutic applications in this context.…”

Section: The Role Of Vitamins Supplementation In Mastitis Alleviationmentioning

confidence: 91%

Enhancing bovine immune, antioxidant and anti-inflammatory responses with vitamins, rumen-protected amino acids, and trace minerals to prevent periparturient mastitis

Khan,

Huang,

Kou

et al. 2024

Front. Immunol.

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Mastitis, the inflammatory condition of mammary glands, has been closely associated with immune suppression and imbalances between antioxidants and free radicals in cattle. During the periparturient period, dairy cows experience negative energy balance (NEB) due to metabolic stress, leading to elevated oxidative stress and compromised immunity. The resulting abnormal regulation of reactive oxygen species (ROS) and reactive nitrogen species (RNS), along with increased non-esterified fatty acids (NEFA) and β-hydroxybutyric acid (BHBA) are the key factors associated with suppressed immunity thereby increases susceptibility of dairy cattle to infections, including mastitis. Metabolic diseases such as ketosis and hypocalcemia indirectly contribute to mastitis vulnerability, exacerbated by compromised immune function and exposure to physical injuries. Oxidative stress, arising from disrupted balance between ROS generation and antioxidant availability during pregnancy and calving, further contributes to mastitis susceptibility. Metabolic stress, marked by excessive lipid mobilization, exacerbates immune depression and oxidative stress. These factors collectively compromise animal health, productive efficiency, and udder health during periparturient phases. Numerous studies have investigated nutrition-based strategies to counter these challenges. Specifically, amino acids, trace minerals, and vitamins have emerged as crucial contributors to udder health. This review comprehensively examines their roles in promoting udder health during the periparturient phase. Trace minerals like copper, selenium, and calcium, as well as vitamins; have demonstrated significant impacts on immune regulation and antioxidant defense. Vitamin B12 and vitamin E have shown promise in improving metabolic function and reducing oxidative stress followed by enhanced immunity. Additionally, amino acids play a pivotal role in maintaining cellular oxidative balance through their involvement in vital biosynthesis pathways. In conclusion, addressing periparturient mastitis requires a holistic understanding of the interplay between metabolic stress, immune regulation, and oxidative balance. The supplementation of essential amino acids, trace minerals, and vitamins emerges as a promising avenue to enhance udder health and overall productivity during this critical phase. This comprehensive review underscores the potential of nutritional interventions in mitigating periparturient bovine mastitis and lays the foundation for future research in this domain.

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Serum Vitamin D Is Associated with Antioxidant Potential in Peri-Parturient Cows

Cited by 8 publications

References 63 publications

The Impact of Cholecalciferol on the Sex Hormones Level and Serum Biochemical Indices in Rat Males With Experimental Gonadopathy

The Impact of Cholecalciferol on the Sex Hormones Level and Serum Biochemical Indices in Rat Males With Experimental Gonadopathy

Managing retained placenta in first-parity doe and administering vitamin A, D, and E as supportive treatment

Enhancing bovine immune, antioxidant and anti-inflammatory responses with vitamins, rumen-protected amino acids, and trace minerals to prevent periparturient mastitis

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