Serum YKL-40 Levels and Chitotriosidase Activity in Patients with Beta-Thalassemia Major

Musumeci, Maria; Caruso, Vincenzo; Medulla, Emilia; Torrisi, Venerando; Migale, Roberta; Angeletti, Silvia; Musumeci, Salvatore

doi:10.1155/2014/965971

Cited by 3 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chitin is a long-chain polysaccharide, which is the major constituent of bacteria cell walls, the sheaths of parasitic nematodes and fungi [6]. In mammals, despite lacking endogenous chitin, several chitinase-like proteins have been determined, though their function is not fully understood [7]. Humans and mice express two active chitinases, namely chitotriosidase (CHIT1) and acidic mammalian chitinase (AMCAse), and at least three chitinase-like proteins (CLPs), namely YKL-40 (chitinase 3-like protein 1), YKL-39 (chitinase 3-like protein 2) and oviductin [8].…”

Section: Introductionmentioning

confidence: 99%

Serum chitotriosidase and YKL-40 in acute pancreatitis: Reliability as prognostic marker for disease severity and correlation with inflammatory markers

Çetin¹,

Kamış²,

ÇETİN³

et al. 2021

Turk J Med Sci

View full text Add to dashboard Cite

Background: Chitotriosidase and YKL-40, also called chitinase 3-like protein 1, are homologs of family 18 glycosyl hydrolases, secreted by human macrophages and granulocytes under inflammatory conditions. Although increased levels of chitotriosidase and YKL-40 are linked with several inflammatory diseases, the physiological utility of these two enzymes is still not fully characterized. This study aims to analyze the serum YKL-40 and chitotriosidase levels of acute pancreatitis patients to assess whether their activity correlates with acute pancreatitisand its severity.Methods: Chitotriosidase and YKL-40 levels, along with routine laboratory parameters, were determined from the serum samples of 41 acute pancreatitis patients, at both onset and remission (male/female: 22/19), and 39 healthy subjects (male/female: 19/20). The Modified Glasgow Prognostic Score was used to predict the severity of the disease, and a correlation analysis was performed between study variables.Results: A statistically significant increase in both chitotriosidase and YKL-40 levels was observed in acute pancreatitis patients compared to healthy controls (P < 0.001). Higher levels of YKL-40, chitotriosidase and C-reactive protein were found in patients with acute pancreatitis at onset than in remission. The correlation analysis showed a statistically significant association between YKL-40 and chitotriosidase (p = 0.039, r = 0.323). The cut-off point for YKL-40, for detecting acute pancreatitis, was 60.3 with a sensitivity and specificity of 84.9% and 84.6% (AUC: 0.890). The optimum cut-off point for chitotriosidase, for detecting acute pancreatitis, was 33.5 with a sensitivity and specificity of 79.5% and 78.4% (AUC: 0.899). Conclusion:Elevated YKL-40 and chitotriosidase levels in acute pancreatitis patients demonstrate the importance of possible macrophage involvement in the pancreatic microenvironment during acute pancreatitis progression.

show abstract

Section: Introductionmentioning

confidence: 99%

Serum chitotriosidase and YKL-40 in acute pancreatitis: Reliability as prognostic marker for disease severity and correlation with inflammatory markers

Çetin¹,

Kamış²,

ÇETİN³

et al. 2021

Turk J Med Sci

View full text Add to dashboard Cite

show abstract

“…Indeed, most of the work reported on this enzyme focused on the association of its expression with various diseases. Hence, HCHT is used as a biomarker for the diagnostic of Gaucher disease, sarcoidosis, glucose intolerance, β-thalassemia and World Trade Center lung injury 16 – 18 .…”

Section: Introductionmentioning

confidence: 99%

Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function

Crasson

Courtade

Léonard

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Chitin is an important structural component of numerous fungal pathogens and parasitic nematodes. The human macrophage chitotriosidase (HCHT) is a chitinase that hydrolyses glycosidic bonds between the N-acetyl-D-glucosamine units of this biopolymer. HCHT belongs to the Glycoside Hydrolase (GH) superfamily and contains a well-characterized catalytic domain appended to a chitin-binding domain (ChBDCHIT1). Although its precise biological function remains unclear, HCHT has been described to be involved in innate immunity. In this study, the molecular basis for interaction with insoluble chitin as well as with soluble chito-oligosaccharides has been determined. The results suggest a new mechanism as a common binding mode for many Carbohydrate Binding Modules (CBMs). Furthermore, using a phylogenetic approach, we have analysed the modularity of HCHT and investigated the evolutionary paths of its catalytic and chitin binding domains. The phylogenetic analyses indicate that the ChBDCHIT1 domain dictates the biological function of HCHT and not its appended catalytic domain. This observation may also be a general feature of GHs. Altogether, our data have led us to postulate and discuss that HCHT acts as an immune catalyser.

show abstract

“…In everyday clinical practice, CHIT1 is used as a circulating biomarker for monitoring the efficacy of various therapeutic approaches in Gaucher's disease where is considered to be a surrogate serum biomarker of the tissue lipid-laden macrophages known as Gaucher cells [40,41]. Elevated CHIT1 was also observed in various other disorders characterized by nonspecific macrophage activation and expansion, such as β-thalassemia [42,43], Plasmodium falciparum malaria [44], sarcoidosis [45][46][47] and Wegener's granulomatosis [48].…”

Section: Introductionmentioning

confidence: 99%

Serum chitotriosidase: a circulating biomarker in polycythemia vera

et al. 2018

View full text Add to dashboard Cite

show abstract

Serum YKL-40 Levels and Chitotriosidase Activity in Patients with Beta-Thalassemia Major

Cited by 3 publications

References 30 publications

Serum chitotriosidase and YKL-40 in acute pancreatitis: Reliability as prognostic marker for disease severity and correlation with inflammatory markers

Serum chitotriosidase and YKL-40 in acute pancreatitis: Reliability as prognostic marker for disease severity and correlation with inflammatory markers

Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function

Serum chitotriosidase: a circulating biomarker in polycythemia vera

Contact Info

Product

Resources

About