Serum β-nerve growth factor concentrations in pregnant female, nonpregnant female, and male cynomolgus monkeys

Neubert, Hendrik; Clark, Tracey; Deng, Shibing; Hurst, Susan

doi:10.1097/wnr.0000000000000187

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…This increase was greater than the observed variability established during assay qualification. Previous studies have shown small, non-significant changes in circulating mature NGF levels during human gestation 59 , 60 and while the data contained in this study does show a small yet significant increase in tNGF over gestation, it is in general agreement with values published previously 48 , 59 .…”

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 93%

“…Previous work using the LCMS assay for NGF demonstrated a large and continuous increase in NGF expression (up to ~ 78×) during gestation in cynomolgus monkeys, with no difference in circulating levels between male and the non-pregnant female control populations 48 . Though a large increase in circulating tNGF levels has been shown in cynomolgus monkeys 48 , this phenomenon has not been reported in human pregnancy which seems to point towards species specific differences during pregnancy. While a balance of systemic NGF levels are important for optimal pregnancy progression, the NGF mRNA expression in human normal pregnancy versus spontaneous abortive patients from isolated trophoblasts showed a ~ 2 to 8 fold increase in the latter.…”

Section: Introductionmentioning

confidence: 93%

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Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry

Walsh

Palandra

Goihberg

et al. 2023

Sci Rep

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Abstractβ-Nerve growth factor (NGF) is a neurotrophin that plays a critical role in fetal development during gestation. ProNGF is the precursor form of NGF with a distinct biological profile. In order to investigate the role of NGF and proNGF in pregnant human females, a sensitive and selective immunoaffinity liquid chromatography-tandem mass spectrometry assay was developed and qualified to simultaneously measure the levels of total NGF (tNGF; sum of mature and proNGF) and proNGF using full and relative quantification strategies, respectively. The assay was used to determine serum tNGF and proNGF levels in the three gestational trimesters of pregnancy and in non-pregnant female controls. Mean tNGF ± SD were 44.6 ± 12.3, 42.6 ± 9.3, 65.4 ± 17.6 and 77.0 ± 17.8 pg/mL for non-pregnant, first, second, and third trimesters, respectively, demonstrating no significant increase in circulating tNGF between the control and the first trimester, and a moderate yet significant 1.7-fold increase through gestation. proNGF levels during the first trimester were unchanged compared to control. In contrast to tNGF, however, proNGF levels during gestation remained stable without significant changes. The development of this sensitive, novel immunoaffinity duplexed assay for both tNGF and proNGF is expected to enable further elucidation of the roles these neurotrophins play in human pregnancy as well as other models.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 93%

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Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry

Walsh

Palandra

Goihberg

et al. 2023

Sci Rep

Self Cite

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“…Tometten et al (2005) reported that NGF is mandatory for the success of pregnancy as it constitutes a functional link between the nervous, immune and endocrine systems and translating environmental or endocrine signals (Tometten et al, 2005). It has been reported that higher NGF levels in pregnancy which are progressive as trimester progresses in monkeys as compared to non pregnant monkeys (Neubert et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Maternal nerve growth factor levels during pregnancy in women with preeclampsia: A longitudinal study

D'Souza

Kilari

Pisal

et al. 2015

Intl J of Devlp Neuroscience

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Preeclampsia (PE) is characterized by hypertension and proteinuria. Improper development of the placenta due to altered angiogenesis is the main culprit in PE. Nerve growth factor (NGF) is an angiogenic factor which is expressed and localized in the placenta. Our earlier cross sectional study has shown altered NGF levels at delivery in women with PE. However, there are no studies on NGF levels in PE early in pregnancy before manifestation of the disease. Thus, there is a need to examine the role of NGF in vascular development during different stages of gestation in PE. A longitudinal study was carried out where pregnant women were enrolled from two major hospitals from Pune, Bharati hospital and Gupte hospital. They were followed at three different time points [16-20 weeks (T1), 26-30 weeks (T2) and at delivery (T3)] during pregnancy and maternal blood at every time point and cord blood at delivery was collected and processed. This study included normotensive women (n=88) and women with PE (n=48). NGF levels were measured from maternal and cord plasma using the Emax Immuno Assay System (Promega). The data was analyzed using the SPSS/PC+ package (Version 20.0, Chicago, IL, USA). Maternal NGF levels did not change at all time points while cord NGF levels were higher (p<0.05) in women with PE. Further, maternal NGF levels were negatively associated with blood pressure while cord NGF levels were positively associated with baby head circumference. Our data suggests that there may possibly be a compensatory role for NGF in the foeto-placental circulation in PE.

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Developmental toxicity assessment of tanezumab, an anti-nerve growth factor monoclonal antibody, in cynomolgus monkeys (Macaca fascicularis)

Bowman

Evans

Cummings

et al. 2015

Reproductive Toxicology

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Serum β-nerve growth factor concentrations in pregnant female, nonpregnant female, and male cynomolgus monkeys

Cited by 5 publications

References 13 publications

Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry

Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry

Maternal nerve growth factor levels during pregnancy in women with preeclampsia: A longitudinal study

Developmental toxicity assessment of tanezumab, an anti-nerve growth factor monoclonal antibody, in cynomolgus monkeys (Macaca fascicularis)

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