Sesame Oil. IV. Determination of free and bound sesamol

Abstract

SummaryThe Villavecchia test was adapted for the quantitative determination of sesamol in sesame oil. Of the several aromatic aldehydes tested, furfural, in the presence of aqueous sulfuric acid (sp. gr. 1.37 at 15°C.), proved to be the most suitable for the quantitative measurement of the color produced with sesamol.The oil, dissolved in iso‐octane (10 g. per 100 ml.), is shaken with dilute aqueous‐alcoholic potassium hydroxide to remove the free, but not the bound sesamol. The modified Villavecchia test is applied to a) the original iso‐octane solution of the oil, b) the iso‐octane solution after extraction with alkali, and/or c) the aqueous‐alcoholic extract. The optical densities, read at a wavelength of 518 mμ, correspond to the total bound, and free sesamol, respectively.The method was applied to the determination of free, bound, and total sesamol in a number of crude, refined, bleached, and deodorized sesame oils. Agreement between the values for total and free plus bound sesamol was close. Four crude sesame oils of different origins had total contents of sesamol ranging from 0.13 to 0.17%, of which nearly all was in the bound form. The yields of added sesamol varied from 95 to 106%.

Effect of endogenous antioxidants of sesame seeds and sesame oil to the thermal stability of edible vegetable oils

“…The method of Budowski et al (1950) was used for the determination of sesamol. Phenolic compounds were determined using the FolineCiocalteau reagent.…”

Neuroprotective Effect of Sesame Seed Oil in 6-Hydroxydopamine Induced Neurotoxicity in Mice Model: Cellular, Biochemical and Neurochemical Evidence

“…This process was repeated three times using fresh solvent each time to extract most of the oils from the ground seeds. Free fatty acids were determined by Gas Liquid Chromatography (GLC) as per the protocol of Fazli et al [23] and sesamin and sesamol content were analyzed according to method of Budowski et al [24] (Data has already been published in our earlier study Ahmad et al [11]). Control (fat free) diet was provided by Animal House Facility, Hamdard University, New Delhi, India.…”

Effect of dietary sesame oil as antioxidant on brain hippocampus of rat in focal cerebral ischemia

“…This process was repeated three times using fresh solvent each time to extract most of the oils from ground seeds. Free fatty acids were determined by Gas Liquid Chromatography (GLC) as per the protocol of Fazli et al (2005) and sesamin and sesamol content were analyzed according to the method of Budowski et al (1950) (Table 1).…”