Sesbania javanica Miq leaf extracts increase Balb/c 3T3 fibroblast cell migration

Abstract

Sesbania javanica Miq. is widely distributed in canals throughout Thailand and its flowers are commonly consumed in Thailand. Sesbania javanica floral extracts have previously been demonstrated to have antimutagenic activity, but information on the bioactivity and beneficial properties of other plant parts, such as the leaf, remains limited. In this study, the induction of cell proliferation and wound-healing activity of DMSO extracts from flowers and leaves of S. javanica was evaluated. A high concentration (1:25 and 1:50 dilutions) of all extracts was cytotoxic to Balb/c 3T3 fibroblast cells. A low concentration (1:500 and 1:1000 dilutions) of extracts A, B, and C (extracted from leaves) significantly promoted cell viability after treatment of the fibroblast cells for 24 or 48 h. The 1:500 and 1:1000 dilutions of extracts B and C were selected for a scratch assay to assess their wound-healing activity and significantly decreased the wound area after treatment for 24 h. These findings provide evidence that leaf extracts from S. javanica have potential utility for the treatment of wounds.