SESQUITERPENE LACTONS FROM AERIAL PARTS OF ARTEMISIA CAMPESTRIS (var. MARSCHALLIANA) SPRENG.

Abstract

About 500 species of wormwood are known in the world. In the flora of Azerbaijan, this genus is represented by 42 taxa. The main chemicals in wormwood are shown to be sesquiterpene lactones, coumarins, steroids, and essential oils. From the aerial parts of Artemisia campestris (var. Marschalliana) Spreng., collected during the phenological phase of budding, an acetone extract was obtained, which, after removal of the solvent, was subjected to the column chromatography in neutral alumina oxide (II degree of activity) and eluted graduality with hexane, benzene, chloroform and their mixtures of increasing polarity. As a result of chromatographic separation, two sesquiterpene lactones of the eudesmann type were isolated - α-santonin (substance I) with m.p. 170–172 °С and artemisin (substance II) m.p. 201–203 °С. Moreover, an acetyl derivative of artemisin (substance III) with m.p. 196–198°C, the identification of which was carried out on the basis of physicochemical and spectral data (UV, IR, and 1H, 13C, 13C Dept 45, 13C Dept 90, 13C Dept 135 NMR spectra was obtained. Substances I and II are isolated from Artemisia campestris (var. Marschalliana) Spreng. for the first time.

IDENTIFICATION OF Α-Santonin IN ARTEMISIA MARSCHALLIANA SPRENG. COLLECTED IN AZERBAIJAN

“…(J=9.9 Hz, H-1) and 6.24 ppm (J=9.9 Hz, H-2). The SSCC of the olefinic protons of the disubstituted double bond is 9.9 Hz [Kerimli et al, 2022] (Fig. 2).…”

“…15.0 g of the obtained green resin was dissolved in 50 ml of chloroform and chromatographed on a column of Al 2 O 3 neutral II degree of activity (h=120, d=3.5 cm) was eluted with hexane (30 fraction); a mixture of hexane/benzene in the ratio 8:2, 7:3, 6:4, 5:4, 1:1 (50 fraction); benzene (20 fraction) [Kerimli et al, 2022].…”