Sesquiterpenes with Hepatoprotective Activity from Cnidium monnieri on Tacrine-Induced Cytotoxicity in Hep G2 Cells

Abstract

Bioassay-guided fractionation of the EtOH extract of Cnidium monnieri (Apiaceae) furnished two hepatoprotective sesquiterpenes, torilin (1) and torilolone (2), together with a new derivative, 1-hydroxytorilin (3). Compounds 1 and 2 showed hepatoprotective effects on tacrine-induced cytotoxicity in human liver-derived Hep G2 cells. The EC50 values of compounds 1 and 2 were 20.6 +/- 1.86 (P < 0.01) and 3.6 +/- 0.1 (P < 0.01) microM, respectively. Silybin as a positive control showed an EC50 value of 69.0 +/- 3.4 microM.

Hepatoprotective Compounds of the Roots of Cudrania tricuspidata on Tacrine-Induced Cytotoxicity in Hep G2 Cells

“…2) During the course of our continuing screening studies [3][4][5][6][7] for natural hepatoprotective agents, we found that the CHCl 3 -soluble fraction of the MeOH extract of the root barks of Cudrania tricuspidata BUREAU (Moraceae), which is one of the traditional Chinese medicines used for the treatment of lumbago, hemoptysis, and contusion, 8) showed promising hepatoprotective activity. We describe the isolation and identification of the hepatoprotective constituents in C. tricuspidata root barks.…”

Guaiane Sesquiterpenoids Isolated from the Fruits of Torilis japonica and Their Cytotoxic Activity

“…A difference of 16 mass units compared to 4 further supported the inference. The position of this OH group was determined to be at C (6) H-COSY data, and the partial structure C(14)ÀC(10)ÀC(9)ÀC(8)ÀC(7)ÀC(6) was further confirmed by the HMBCs (Fig. 2).…”

“…In our phytochemical investigation on the species, we obtained two new guaiane sesquiterpenoids, (1b,8b)-11-(acetyloxy)-1,8-dihydroxyguai-4-en-3-one 1 ) (5) and (1a,6b)-1,6-dihydroxytorilin 1 ) (6) (Fig. 1), together with four known sesquiterpenes, torilin (1) [4], torilolone (2) [1], (1b)-1-hydroxytorilin (3) [5], and (1a)-1-hydroxytorilin (4) [6]. In this phytochemical study, daucosterol [7], friedelin [8], and epifriedelanol [8] were also identified as constituents of the plant for the first time.…”

In Vitro hepatoprotective compounds from Suaeda glauca

“…In the present study, an immortalized human liver-derived cell line, Hep G2 was employed for screening of hepatoprotective agents against tacrine-induced cytotoxicity, since the cell line retains many cellular functions, and is known to be comparable with rat primary hepatocytes in tacrine-induced cytotoxicity (Halliwell, 1994). During the course of our continuing screening studies for natural hepatoprotective agents (An et al, 2005;2006;Park et al, 2004;Oh et al, 2002), we found that the EtOAc-soluble fraction of the MeOH extract of Suaeda glauca (Bunge) Bunge had been shown to possess hepatoprotective activity. S. glauca belongs to Chenopodiaceae and is a marine plant widely distributed in Asia (Kitamura and Murata, 1987).…”