Sestrin2 and Beclin1 levels in Polycystic Ovary Syndrome

Abstract

Background Sestrin2 and beclin1 are two newly found proteins that have essential roles in autophagy. This study attempted to evaluate the plasma concentrations of sestrin2 and beclin1 in women with polycystic ovary syndrome (PCOS) and healthy controls and to explore the clinical value of these proteins as novel biomarkers for PCOS. Methods In this case‐control study, plasma levels of sestrin2 and beclin1, fasting blood sugar (FBS), lipid profile, insulin, and androgens were evaluated in 63 women (31 patients and 32 controls). Sestrin2 and beclin1 levels were determined using enzyme‐linked immunosorbent assay (ELISA). Descriptive statistics, correlation coefficients, logistic regression, and ROC curve analyses were used in this study. Results Plasma sestrin2 levels of the subjects with PCOS (40.74 [24.39–257.70]) were significantly lower than those of healthy subjects (255.78 [25.46–528.66]; p‐value = 0.040). ROC curve analysis showed that a cutoff value of 420.5 ng/L had an appropriate sensitivity (83.87%) and specificity (46.88%) for discriminating individuals with and without PCOS, with the area under the curve (95% CI) of 0.648 (0.518 to 0.764), p = 0.036. There were no statistically significant differences between the two groups concerning plasma levels of beclin1, biochemical parameters, blood pressure, and anthropometric features. Conclusion Our findings highlight the dysregulation of sestrin2 as a marker of autophagy in PCOS and its potential usefulness as a novel biomarker for PCOS. Further research is needed to better understand the role of this protein in the pathophysiology of PCOS and its value as a diagnostic tool for the evaluation of PCOS patients.

Fahr’s disease with neuropsychiatric symptoms and intermittent course: a case report

“…However, extensive laboratory tests, including serological tests and cerebrospinal uid analysis, effectively ruled out these infectious causes. The investigation also took into account metabolic disorders, which can be associated with neuropsychiatric symptoms and calci cations (2,9). Conditions like Wilson's disease, hypoparathyroidism, and mitochondrial disorders were evaluated but ultimately excluded through appropriate laboratory tests such as copper studies, parathyroid hormone levels, and liver function tests.…”

Evaluation of sestrin 2 and tribbles homolog 3 levels in obese and nonobese women with polycystic ovary syndrome

“…However, inconsistencies are found in the studies. In a recent study on women with PCOS, plasma sestrin 2 levels were shown to be significantly lower than in healthy controls [ 38 ], and higher in another study [ 16 ]. In this study, nonobese and obese women with PCOS were separated and compared in terms of sestrin 2 levels.…”

“…It has been suggested that disorders related to autophagy, a stress-induced lysosome-mediated degradation process, cause insulin resistance. In addition, dysregulation of autophagy was observed in the ovarian granulosa cells and endometria of women with PCOS [ 8 ]. It is also thought to be associated with increased oxidative stress and insulin resistance in PCOS [ 9 ].…”

“…Sestrin 2 reduces IR through the regulation of glucose and lipid homeostasis [ 15 ]. One study in patients with PCOS reported that plasma sestrin 2 levels were significantly lower than those in healthy controls [ 8 ]. However, in another recent study by Beskel et al, sestrin levels were found to be high as an indicator of oxidative stress in women with PCOS [ 16 ].…”