Set of stress biomarkers as a practical tool in the assessment of multistress effect using honeybees from urban and rural areas as a model organism: a pilot study

Abstract

A decrease among honey bee populations (Apis mellifera) in the traditional apiaries has been observed in recent years. In light of this negative phenomenon, urban beekeeping seems to be an appropriate alternative solution for the bee population in reducing the toxic effects of a large number of pesticides that are commonly used in agricultural ecosystems. Despite the rapid development of urban beekeeping, there is little information regarding the different aspects of the defense effectiveness of bees from the urban and rural areas. The study was aimed to show whether honey bees from these two locations differ in the level of the valuable biomarkers of stress exposure helpful in establishing which bees, from urban or rural areas, are under greater environmental pressure. For this purpose, foragers from an urban rooftop apiary and a traditional rural apiary were collected. The chosen biomarkers were measured in various tissues of bees. The activity of glutathione S-transferase and acetylcholinesterase, the level of total antioxidant capacity, heat shock protein 70 (Hsp70), and defensin were selected for the analyses. In our opinion, the Hsp70 and defensin levels seemed to be important in the indication of urban multistress factors. The higher level of heat shock proteins and defensins in tissues/organs of bees from the urban apiary—in the gut (an increase, respectively, 92% and 7.3%) and fat body (an increase, respectively, 130% and 7.8%), known as targets of environmental toxins, pointed out the urban environment as highly stressful at both the individual and colony levels. In turn, high total antioxidant capacity was measured in the guts of honey bees from rural area (an increase 107%). Such a situation suggests a different mechanism of defense and specificity of rural and urban environmental stressors and also honey bees foraging activity.

Review on Sublethal Effects of Environmental Contaminants in Honey Bees (Apis mellifera), Knowledge Gaps and Future Perspectives

“…An inhibition of some antioxidant stress biomarkers (GSH, SOD, CAT, GST) was also observed in specimens collected from anthropized areas [ 56 ]. Nicewicz et al (2020) [ 141 ] observed the importance of defensin and HSP70 levels as indicators of urban multistress both at individual and colony levels. Further studies are needed to investigate the ecotoxicological status of honey bee colonies.…”

Antioxidant capacity of honey from the urban apiary: a comparison with honey from the rural apiary

“…Differences in water content in honey samples may result, for example, from temperature differences in the location of the apiary. Hives from urban apiaries are usually located on the roofs of buildings, which favors the increase in the already high temperature in the city (heat island effect) 12 . Higher temperature tends to the evaporation of water during honey maturation in bee colonies.…”

Effect of Rearing in Small-Cell Combs on Activities of Catalase and Superoxide Dismutase and Total Antioxidant Capacity in the Hemolymph of Apis mellifera Workers

“…The interaction between different antioxidants provides better protection against the effects of ROS than could any single compound. The oxidative changes and efficiency of the entire antioxidant system in bees are quantified in the total antioxidant capacity (TAC) analysis [ 15 , 16 , 17 ]. Intracellular TAC is mainly attributed to the enzyme system, whereas plasma TAC is mainly related to dietary low molecular weight antioxidants.…”