Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1

Deng, Xiao-Yue; Iwagawa, Toshiro; Fukushima, Masaya; Suzuki, Yutaka; Watanabe, Sumiko

doi:10.1167/iovs.62.6.1

Cited by 5 publications

(6 citation statements)

References 59 publications

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“…To construct the plasmid expressing SETD5 lacking the interaction domain with HDAC3 and PAF1 complexes, 1257 serine was mutated to a stop codon by using inverse PCR-based KOD Mutagenesis Kit (SMK-101; Toyobo, Osaka, Japan). The plasmids expressing shRNA were constructed as described previously [10]. The target sequences of shRNA were designed by using SIDIRECT (http://sidirect2.rnai.jp) and are as follows: shSet-d2_1st, 5 0 -aagatattgtatctaatagtttg-3 0 ; shSetd2_2nd, 5 0 -aagaa atcttcacaatttgaagg-3 0 ; shSetd5_1st, 5 0 -atgaatatagtaactgtaatt ac-3 0 ; shSetd5_2nd, 5 0 -ctgctgttaagggtacattgtta-3 0 .…”

Section: Dna Constructionmentioning

confidence: 99%

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Setd5, but not Setd2, is indispensable for retinal cell survival and proliferation

et al. 2022

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Trimethylation of histone H3 at lysine 36 (H3K36me3) is associated with active transcription. We used mouse retinal explant cultures and shRNA to investigate the roles of Setd2 and Setd5, which encode H3K36me3 methyltransferases, in retinal development. We found that shSetd5 caused abnormal retinal structures and reduced rods and M€ uller cells, whereas shSetd2 did not cause any abnormalities. The mutant SETD5 lacking the SET domain failed to reverse the phenotypes observed in the shSetd5-expressing retinas, while SETD5S1257*, which does not interact with HDAC3 and PAF1 complexes, rescued proliferation, but not apoptosis, induced by shSetd5. Taken together, we found that Set-d5, but not Setd2, is essential for sustaining retinal cell survival and proliferation, and the SET domain of SETD5 is pivotal for both functions.

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Section: Dna Constructionmentioning

confidence: 99%

“…The role of histone methylation during retinal development has been investigated by knockout or knockdown of enzymes that catalyze histone methylation and demethylation in mouse retinas. Ezh2 (Kmt6a), Utx (Kdm6a), Jmjd3 (Kdm6b) and Setd1a (Kmt2f) all have roles in the retinal development [8][9][10].…”

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confidence: 99%

Setd5, but not Setd2, is indispensable for retinal cell survival and proliferation

et al. 2022

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“…Key transcription factors for photoreceptor development, including NRL, CRX, and OTX2, are also involved in H3K4 methylation during retinal development (Kizilyaprak et al, 2010;Ueno et al, 2016;Iwagawa and Watanabe, 2019). In a retinal explant model, depletion of the histone methyltransferase SETD1A produced a hypomethylation pattern of H3K4, resulting in an increase in apoptotic cells and a significant decrease in proliferating cells, as well as a decrease in the number of rod photoreceptor cells during late retinal development (Deng et al, 2021). These findings indicate that the terminal differentiation of retinal cells involves the dynamic regulation of histone methylation, especially the methylation of H3K4.…”

Section: Regulation Of Photoreceptor Differentiation By Histone Methy...mentioning

confidence: 99%

The role of epigenetic methylation/demethylation in the regulation of retinal photoreceptors

Zhou

Zhang

et al. 2023

Front. Cell Dev. Biol.

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Photoreceptors are integral and crucial for the retina, as they convert light into electrical signals. Epigenetics plays a vital role in determining the precise expression of genetic information in space and time during the development and maturation of photoreceptors, cell differentiation, degeneration, death, and various pathological processes. Epigenetic regulation has three main manifestations: histone modification, DNA methylation, and RNA-based mechanisms, where methylation is involved in two regulatory mechanisms-histone methylation and DNA methylation. DNA methylation is the most studied form of epigenetic modification, while histone methylation is a relatively stable regulatory mechanism. Evidence suggests that normal methylation regulation is essential for the growth and development of photoreceptors and the maintenance of their functions, while abnormal methylation can lead to many pathological forms of photoreceptors. However, the role of methylation/demethylation in regulating retinal photoreceptors remains unclear. Therefore, this study aims to review the role of methylation/demethylation in regulating photoreceptors in various physiological and pathological situations and discuss the underlying mechanisms involved. Given the critical role of epigenetic regulation in gene expression and cellular differentiation, investigating the specific molecular mechanisms underlying these processes in photoreceptors may provide valuable insights into the pathogenesis of retinal diseases. Moreover, understanding these mechanisms could lead to the development of novel therapies that target the epigenetic machinery, thereby promoting the maintenance of retinal function throughout an individual’s lifespan.

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“…Tere have been a number of approaches proposed in the literature to extend the framework of DS evidence theory and, in particular, the notion of the mass function within the context of quantum probability construction [25][26][27][28].…”

Section: Quantum-enhanced Mass Functionmentioning

confidence: 99%

Applications of Quantum Probability Amplitude in Decision Support Systems

Payandeh

2023

Applied Computational Intelligence and Soft Computing

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Establishing various frameworks for managing uncertainties in decision-making systems have been posing many fundamental challenges to the system design engineers. Quantum paradigm has been introduced to the area of decision and control communities as a possible supporting platform in such uncertainty management. This paper presents an overview of how a quantum framework and, in particular, probability amplitude has been proposed and utilized in the literature to complement two classical probabilistic decision-making approaches. The first such framework is based in the Bayesian network, and the second is based on an element of Dempster–Shafer (DS) theory using the definition of mass function. The paper first presents a summary of these classical approaches, followed by a review of their preliminary enhancements using the quantum model framework. Particular attention was given on how the notion of probability amplitude is utilized in such extensions to the quantum-like framework. Numerical walk-through examples are combined with the presentation of each method in order to better demonstrate the extensions of the proposed frameworks. The main objective is to better define and develop a common platform in order to further explore and experiment with this alternative framework as a part of a decision support system.

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Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1

Abstract: Setd1a plays pivotal roles for the survival and proliferation of retinal progenitors via histone modifications of Uhrf1.

Cited by 5 publications

References 59 publications

Setd5, but not Setd2, is indispensable for retinal cell survival and proliferation

Setd5, but not Setd2, is indispensable for retinal cell survival and proliferation

The role of epigenetic methylation/demethylation in the regulation of retinal photoreceptors

Applications of Quantum Probability Amplitude in Decision Support Systems

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