The river ecosystem in the Hai River Basin (HRB), an important economic region in China, is seriously degraded. With the aim of river restoration in the HRB, we developed a method to assess the river's ecological status and conducted a preliminary application of the method.The established method was a predictive model, which used macroinvertebrates as indicator organisms. The river's ecological status was determined by calculating the ratio of observed to expected values (O/E). The method included ecoregionalization according to natural factors, and the selection of reference sites based on combinations of habitat quality and macroinvertebrate community. Macroinvertebrate taxa included Insecta, Crustacea, Gastropoda, and Oligochaeta, with 39 families and 95 genera identified in the HRB. The HRB communities were dominated by pollution tolerant taxa, such as Lymnaeidae, Chironomus, Limnodrilus, Glyptotendipes, and Tubifex. The average ShannonWiener index was 1.40 ± 0.5, indicating a low biodiversity. In the river length of 3.31 × 10 4 km, 55% of the sites were designated poor, with a bad ecological status. Among nine secondary river systems, Luan and Zi-ya had the best and worst river conditions, respectively. Only 17 reference site groups were selected for river management in the 41 ecoregions examined. This study lays the foundation for river restoration and related research in the HRB, and we anticipate further developments of this novel method.