Setting the reference for the use of Chironomus sancticaroli (Diptera: Chironomidae) as bioindicator: Ontogenetic pattern of larval head structures

Rebechi, Débora; Navarro-Silva, Mário Antônio

doi:10.1590/s1984-46702012000200009

Cited by 6 publications

(5 citation statements)

References 18 publications

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“…Furthermore, we suggest an alternative way to ascertain the larval instars: the variation in the number of striae present on the ventromental plates. This method makes it unnecessary to use morphometric data, as in the present study (REBECHI & NAVARRO-SILVA 2012). Beyond the length of the head capsule from ventral view, measurements of the antennae, mandibles, mentum and ventromental plates are efficient alternatives for the determination of the larval instars, as found by FORD (1959) for antennae and mandibles of Clinotanypus nervosus Meigen, 1818; Anatopynia trifascipennis Zetterstedt, 1838 and Procladius choreus Meigen, 1804 and by FROUZ et al (2002) for the mentum of Chironomus crassicaudatus Malloch, 1915 andGlyptotendipes paripes (Edwards, 1929).…”

Section: Discussionmentioning

confidence: 94%

Determination of larval instars in Chironomus sancticaroli (Diptera: Chironomidae) using novel head capsule structures

Richardi¹,

Rebechi²,

Aranha

et al. 2013

Zoologia (Curitiba)

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Determining the age composition of a population is important when conducting ecological, taxonomic and environmental assessments. Morphometric measurements of the head capsule, especially the length and width, are widely used in the identification of insect instars, but alternative ways for determining the age of insects can expand the options for the analysis of field and laboratory populations. This study evaluated the morphometry of the antennae, mandibles, mentum and ventromental plates to discriminate among the four larval instars of Chironomus sancticaroli Strixino & Strixino, 1981. The larvae were reared in the laboratory under constant temperature (25°C) and photoperiod of 12/12 hours for 19 days, with a supply of food. Fifteen larvae were removed randomly every day, cleared in KOH, and slide-mounted, and had the antennae, mandibles, mentum and ventromental plates measured. The dimensions of the four structures studied allowed us to statistically distinguish each of the four larval instars. The data fit an exponential equation according to the Brooks-Dyar rule, which allows an estimate of the larval instar of specimens collected in the field, even when development takes place under different conditions. The duration of each instar was also obtained from our data, and showed an overlap of instars during development.

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Section: Discussionmentioning

confidence: 94%

Determination of larval instars in Chironomus sancticaroli (Diptera: Chironomidae) using novel head capsule structures

Richardi¹,

Rebechi²,

Aranha

et al. 2013

Zoologia (Curitiba)

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“…Chironomids are good bioindicators of the aquatic environment because they spend most of their life in bottom sediments, where they are exposed to toxic substances such as heavy metals, radioactive substances, pesticides and other xenobiotics, originating from sources such as industrial waste and agricultural runoff. Chironomids have been shown to be valuable models for research on morphological deformity, trophic classification of lakes and paleolimnology, and in toxicity studies (Meregalli et al 2000;De Bisthoven et al 2005;Lee & Choi 2009;Weltje et al 2010;Rebechi & Navarro-Silva 2012). Studies of their behavior and life history, as well as biomonitoring studies, have been carried out at population and ecosystem levels.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of the oxidative system in Chironomidae larvae with contrasting feeding strategies

Szczerkowska-Majchrzak

Jarosiewicz

2020

The European Zoological Journal

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The aim of the present work was to compare the oxidative system in the fourth-instar larvae of four species of Diptera belonging to the family Chironomidae: Chironomus riparius, Paratanytarsus sp., Prodiamesa olivacea and Conchapelopia melanops. These species were selected based on their ecological and biological differences and were assessed in relation to their ecological and biological characteristics. The following parameters of oxidative stress were determined: the levels of total antioxidant capacity (TAC) and reduced glutathione; the activity of the antioxidant enzyme catalase (CAT); the level of malondialdehyde (MDA), a product of lipid peroxidation; and glutathione S-transferase (GST) activity. Results demonstrated significant differences among taxa in the specific activity of most investigated parameters. Prodiamesa olivacea showed the greatest antioxidant protection (activity of CAT, level of reduced glutathione (GSH)) and the highest detoxification activities (activity of GST and level of GSH). Predatory C. melanops was shown to be the most promiscuous biomarker for assessing the total antioxidant activity. We demonstrate that the four chironomid taxa, occupying the same ecosystem, share an important antioxidant enzymatic and non-enzymatic pool, but with oxidative stress parameters differing among the four species.

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“…On the other hand, Dornfeld (2006); evaluating the toxicity of cadmium and copper in C. xanthus assays, observed only 2.0% deformity in control larvae. In laboratory culturing, Rebechi and Silva (2012) observed 9% of mentum deformity in C. sancticaroli (instar II) reared in standard conditions and 48% in III instar, while Viveiros (2012) observed an average of 13% for the same specie (IV instar). Despite these data, the criteria used for field studies should not be applied directly to laboratories tests, because the environmental conditions are different.…”

Section: Resultsmentioning

confidence: 99%

Frequency of mentum deformity in Chironomus sancticaroli (Diptera: Chironomidae) in a laboratory culture

Silva¹,

Marigo²,

Viveiros³

et al. 2019

Rev. ambiente água

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The midge Chironomus sancticaroli (Diptera: Chironomidae) has been used in ecotoxicological tests because it is sensitive to a variety of inorganic pollutants. Among the parameters used to evaluate the toxicity of a substance is the frequency of mentum deformity, which is part of the oral system of this organism. However, there is still no consensus on the baseline level (percentage) of acceptable deformities in laboratory cultures not exposed to pollutants. The determination of this variable is important to ensure the validity of bioassays and to compare cultures from different research and teaching institutions. Once this value is established, it will also be used to monitor the quality of organisms cultured, since factors such as inbreeding could increase the frequency of mentum deformity. Thus, the objective of this study was to quantify the percentage of mentum deformity in the fourth instar of C. sancticaroli larvae from the culture of the Laboratory of Aquatic Ecosystems, at Embrapa Meio Ambiente. The average frequency of mentum deformity obtained was 6,63%. It is believed that factors such as the renewal of the culture with the inclusion of spawns from the laboratories of other institutions, as well as the control of the quality of the dilution water and the sediment of the breeding may have contributed to a low frequency of mentum deformity of the culture observed in this study.

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Setting the reference for the use of Chironomus sancticaroli (Diptera: Chironomidae) as bioindicator: Ontogenetic pattern of larval head structures

Cited by 6 publications

References 18 publications

Determination of larval instars in Chironomus sancticaroli (Diptera: Chironomidae) using novel head capsule structures

Determination of larval instars in Chironomus sancticaroli (Diptera: Chironomidae) using novel head capsule structures

A comparative study of the oxidative system in Chironomidae larvae with contrasting feeding strategies

Frequency of mentum deformity in Chironomus sancticaroli (Diptera: Chironomidae) in a laboratory culture

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