Settlement of<i>Balanus Balanoides</i>: The Effect of Cyprid Antennular Secretion

Yule, A. B.; Walker, Graham C.

doi:10.1017/s0025315400052541

Cited by 77 publications

(45 citation statements)

References 9 publications

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“…The localization of this cue to cuticle means that one mode of detection could be through contact of the cyprid (presumably with the paired antennules) with the cuticle that covers the basal region of the shell plates of the adult barnacle (Bourget 1977). Moreover, we have shown previously that the attachment disc of the cyprid antennules immunostains for the SIPC and that this glycoprotein is deposited on the substratum during the cyprid 'exploratory' walk prior to final settlement (Matsumura et al 1998b;Dreanno et al 2006); these 'footprints' then act as a cue to other cyprids promoting additional settlement (Yule & Walker 1985;Clare et al 1994). The localization of the SIPC in the barnacle cuticle suggests that in addition to functioning as a contact pheromone, this glycoprotein might also act as a waterborne cue (an attractant?)…”

Section: Discussionmentioning

confidence: 95%

Locating the barnacle settlement pheromone: spatial and ontogenetic expression of the settlement-inducing protein complex ofBalanus amphitrite

2006

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Barnacles are prominent members of hard substratum benthic communities and their study has been important to advances in experimental ecology and contemporary ecological theory. Having recently characterized the cue to gregarious settlement of Balanus amphitrite, the settlement-inducing protein complex (SIPC), we use two polyclonal antibodies to examine the tissue distribution and ontogenetic expression of this glycoprotein. These antibodies were raised against two separate peptides located near the N-and C-termini of the SIPC and were used to detect the glycoprotein by western blotting and immunohistochemistry. By in situ hybridization we also show that the SIPC mRNA co-occurs with the expressed glycoprotein in the cuticles of both nauplius and cypris larval stages and the adult. In the larvae, the SIPC is expressed most strongly in the mouthparts and the hindgut of the stage 2 nauplius and in the thoracopods, antennules and bivalved carapace of the cyprid. In adult B. amphitrite, the expressed SIPC is present in protein extracts of the shell and in all organs that are lined by cuticular tissues. We suggest that the SIPC is produced by the epidermal cells that secrete the cuticle and discuss these observations with regard to earlier studies and the role of the SIPC as a contact pheromone.

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Section: Discussionmentioning

confidence: 95%

Locating the barnacle settlement pheromone: spatial and ontogenetic expression of the settlement-inducing protein complex ofBalanus amphitrite

2006

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“…Inhibit~on of cyprid attachment has also been reported for natural multi-species microfilms on polystyrene petri dishes preconditioned in seawater ( M a h et al 1988, 1990, 1992), but those observations are confined to relatively young films, with substrata being preconditioned for only 24 to 120 h. The use of varying, and often large, numbers of larvae in their assays (15 to 200 per dish, Maki et al 1988Maki et al , 1990Maki et al , 1992 renders their data difficult to interpret in view of possible density-related behavioural effects. Yule & Walker (1985) and Clare et al (1994) have raised the possibility of cyprid-cyprid interactions at the time of settlement having important implications for the design of barnacle settlement assays, and the latter recommend utilization of low cyprid densities or even single larvae wherever possible.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory and facilitatory effects of microbial films on settlement of Balanus amphitrite amphitrite larvae

Wieczorek¹,

Clare²,

Todd³

1995

Mar. Ecol. Prog. Ser.

112

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Larvae of the barnacle Balanus amphltr~te amphltrite Darwin (Crustacea Cirnpedia) respond to microbial surface film cues at settlement (l e attachment and metamorphosis) The effects of fllms of varylng ages on the settlement behavlour of B amphitrite cypnds were examined in laboratory cholce assays uslng substrata preconditioned In natural seawater for longer penods (up to 18 d ) than previous studies Furthermore, in this study only known numbers of cyprids (either 1 or 20 larvae) per film treatment were investigated, allowing assessment of possible behavioural interactions between larvae Quantitative analyses of the multi-species films permitted oblective compansons between fllrn age treatments A general trend of a reversal of the effect of filmlng on cypnd settlement response -from ~nhibitory to facllitatory -was noted with increasing film age and isolated cypnds responded similarly to groups of 20 cypnds Microbial abundance and overall blotic diversity were found to be markedly greater in 'older films The fac~litation of cyprid settlement by 'older' fllms contrasts with previously published studies on B a m p l~~t~l t e and may be explained either by the greater ages of f~lms assayed In the present study or qual~tatlve/quantltative differences in fllms between studies

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“…For example, contact with live adults may 15 not be the only way a conspecific cue could influence barnacle settlement in the wild. Cyprids 16 leave conspecific cues on surfaces when adhesive proteins are secreted from their antennules 17 during exploration of the substrate (Yule and Walker, 1985;Clare et al, 1994). Fine-scale 18 observations on distribution of cyprids suggest that they aggregate (Wethey, 1984), which could 19 be a response to secretions from the antennules or another source of conspecific cues.…”

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Causes of decoupling between larval supply and settlement and consequences for understanding recruitment and population connectivity

Pineda

Porri

Starczak

et al. 2010

Journal of Experimental Marine Biology and Ecology

184

100

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Marine broadcast spawners have two-phase life cycles, with pelagic larvae and benthic adults. 2 Larval supply and settlement link these two phases and are crucial for the persistence of marine 3 populations. Mainly due to the complexity in sampling larval supply accurately, many 4 researchers use settlement as a proxy for larval supply. Larval supply is a constraining variable 5 for settlement because, without larval supply, there is no settlement. Larval supply and 6 settlement may not be well correlated, however, and settlement may not consistently estimate 7 larval supply. 8This paper explores the argument that larval supply (i.e., larval abundance near settlement sites) 9 may not relate linearly to settlement. We review the relationship between larval supply and 10 settlement, from estimates and biases in larval supply sampling, to non-behavioral and 11 behavioral components, including small-scale hydrodynamics, competency, gregarious behavior, 12 intensification of settlement, lunar periodicity, predation and cannibalism. Physical and structural 13 processes coupled with behavior, such as small-scale hydrodynamics and intensification of 14 settlement, sometimes result in under-or overestimation of larval supply, where it is predicted 15 from a linear relationship with settlement. Although settlement is a function of larval supply, 16 spatial and temporal processes interact with larval behavior to distort the relationship between 17 larval supply and settlement, and when these distortions act consistently in time and space, they 18 cause biased estimates of larval supply from settlement data. 19Most of the examples discussed here suggest that behavior is the main source of the decoupling 20 between larval supply and settlement because larval behavior affects the vertical distribution of 21 3 larvae, the response of larvae to hydrodynamics, intensification of settlement, gregariousness, 1 predation and cannibalism. Thus, larval behavior seems to limit broad generalizations on the 2 regulation of settlement by larval supply. Knowledge of the relationship is further hindered by 3 the lack of a well founded theoretical relationship between the two variables. 4The larval supply-settlement transition may have strong general consequences for population 5 connectivity, since larval supply is a result of larval transport, and settlement constrains 6 recruitment. Thus, measuring larval supply and settlement effectively allows more accurate 7 quantification and understanding of larval transport, recruitment and population connectivity. 8 9 4

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Settlement ofBalanus Balanoides: The Effect of Cyprid Antennular Secretion

Cited by 77 publications

References 9 publications

Locating the barnacle settlement pheromone: spatial and ontogenetic expression of the settlement-inducing protein complex ofBalanus amphitrite

Locating the barnacle settlement pheromone: spatial and ontogenetic expression of the settlement-inducing protein complex ofBalanus amphitrite

Inhibitory and facilitatory effects of microbial films on settlement of Balanus amphitrite amphitrite larvae

Causes of decoupling between larval supply and settlement and consequences for understanding recruitment and population connectivity

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