Setup for Simultaneous Microwave Heating and Real-Time Spectrofluorometric Measurements in Biological Systems

Kohler, Sophie; Ticaud, Nicolas; Iordache, Maria-Minodora; Moisescu, Mihaela G.; Savopol, Tudor; Lévêque, Philippe; Arnaud‐Cormos, Delia

doi:10.2528/pier13110703

Cited by 8 publications

(3 citation statements)

References 29 publications

(31 reference statements)

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“…In addition, various studies indicated that the actual molecular mechanism for As 2 O 3 -induced toxicity could be related to its triggering of apoptosis in many cell lines such as the SH-SY5Y cell line [ 18 , 19 ] and brain cells, including primary neurons [ 20 ] and glioma cells [ 21 ]. Finally, in a further effort, we also adapted a transverse electromagnetic cell (TEM cell) [ 22 , 23 , 24 ] onto a digital holographic microscope platform to assess whether CW or GSM-modulated 1800 MHz signals at up to 6 W/kg and steady temperature impacted the well characterized autophagic response of serum-deprived HCT116 cells [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Joushomme

Garenne

Dufossée

et al. 2022

IJMS

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It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellular response during RF exposure alone, or during co-exposure to RF and chemical treatments known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). The specific absorption rates (S.A.R.) used were 1.5 and 6 W/kg during DHM experiments and ranged from 5 to 24 W/kg during the recording of cellular impedance. Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant.

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Section: Introductionmentioning

confidence: 99%

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Joushomme

Garenne

Dufossée

et al. 2022

IJMS

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“…It is known that small TEM cells have a high useful frequency band. We have previously used the TEM cell shown in Figure 1 as a delivery system for microwaves [8,9,14,27,28] and for nanosecond electric pulses [17,29,30]. The biological sample was placed in a 35-mm Petri dish centered above the TEM cell lower plate.…”

Section: Opened Tem Cellmentioning

confidence: 99%

“…A 12-mm distance between the septum and the lower plate was chosen to allow inserting a Petri dish below the septum of the TEM cell. For this type of TEM cells where the height is rather small compared to the septum width, the E-field has a good uniformity over the TEM cell height [28]. Due to the size of the microscope objective, an aperture with a diameter of 20 mm was made in the center of the lower TEM cell ground plate to allow real-time microscopic observation.…”

Section: Opened Tem Cellmentioning

confidence: 99%

Electromagnetic Analysis of an Aperture Modified Tem Cell Including an Ito Layer for Real-Time Observation of Biological Cells Exposed to Microwaves

Soueid¹,

Kohler²,

Carr³

et al. 2014

PIER

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Abstract-We propose to analyze the aperture and ITO layer presence of a modified transverse electromagnetic (TEM) cell. This TEM cell can be used to study the potential effects of microwave electromagnetic fields on biological cells. This modified delivery device allows real-time observation of biological cells during exposure. Microscopic observation is achieved through an aperture in the lower wall of the TEM cell that is sealed with a 700-nm film of the transparent conducting material Indium tin oxide (ITO). To determine the device efficiency, numerical and experimental electromagnetic dosimetry was conducted. For assessing the effect of the aperture on the specific absorption rate (SAR) in the exposed sample, a plastic Petri dish containing cell culture medium, full-wave 3-D electromagnetic simulations and temperature measurements were performed. For 1-W input power, the SAR values obtained at 1.8 GHz in the sample exposed in the TEM cell with the sealed or non-sealed aperture of 20-mm diameter were 1.1 W/kg and 23.6 W/kg, respectively. An excellent homogeneity of the SAR distribution was achieved when the aperture was sealed with the ITO layer. The performance of the delivery system was confirmed by microwave exposure and simultaneous observation of living cells.

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Delivery devices for exposure of biological cells to nanosecond pulsed electric fields

Soueid¹,

Dobbelaar

Bentouati³

et al. 2017

Med Biol Eng Comput

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In this paper, delivery devices for nanosecond pulsed electric field exposure of biological samples in direct contact with electrodes or isolated are presented and characterized. They are based on a modified electroporation cuvette and two transverse electromagnetic cells (TEM cells). The devices were used to apply pulses with high intensity (4.5 kV) and short durations (3 and 13 ns). The delivery devices were electromagnetically characterized in the frequency and time domains. Field intensities of around 5, 0.5, and 12 MV m were obtained by numerical simulations of the biological sample positioned in the three delivery devices. Two delivery systems had a homogenous electric field spatial distribution, and one was adapted to permit a highly localized exposure in the vicinity of a needle. Experimental biological investigations were carried out at different field intensities for five cancer cell lines. The results using flow cytometry showed that cells kept polarized mitochondrial membrane but lost plasma membrane integrity following a dose-response trend after exposure to different electric field intensities. Certain cell types (U87, MCF7) showed higher sensitivities to nsPEFs than other lines tested.

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Setup for Simultaneous Microwave Heating and Real-Time Spectrofluorometric Measurements in Biological Systems

Cited by 8 publications

References 29 publications

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Electromagnetic Analysis of an Aperture Modified Tem Cell Including an Ito Layer for Real-Time Observation of Biological Cells Exposed to Microwaves

Delivery devices for exposure of biological cells to nanosecond pulsed electric fields

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