Seven genes from human chromosome 18 map to chromosome 24 in the bovine

Abstract

Bovine sequence tagged sites (STSs) were developed for seven genes and used for synteny mapping with a hybrid bovine × rodent cell line panel. The genes were thymidylate synthase (TYMS), pituitary adenylate cyclase activating peptide (ADCYAP1), and melanocortin-2 receptor (MC2R) from the short arm of human chromosome (HSA) 18 and N-cadherin (CDH2), transthyretin (TTR), gastrin-releasing peptide (GRP), and plasminogen activator inhibitor 2 (PAI2) from the long arm of HSA 18. Primers for these genes were designed with human, ovine, or bovine sequences aligned with a sequence from a second species. The bovine PCR product was cloned, and the fragment was sequenced to verify that the homologous gene was indeed amplified. A second set of bovine-specific PCR primers were developed for each gene from these sequences. These STSs were used for synteny mapping, and all seven genes were syntenic with markers of bovine chromosome (BTA) 24. The concordance with BTA 24 was at least 96.5 % for all genes.

Synthesis of transthyretin by the ependymal cells of the subcommissural organ

“…These components exhibited a pH range from 4.9 to 5.8, very close to the pH range described for TTR from other species (Pettersson et al 1987(Pettersson et al , 1995. To substantiate further that the presence of TTR in bovine SCO cells was attributable to synthesis and not to endocytosis, RNA isolated from the SCO and various adult bovine tissues was subjected to reverse transcription-polymerase chain reaction (RT-PCR) with an amplimer pair for bovine TTR (Larsen and Kirkpatrick 1996). As predicted, a 121-bp PCR product was efficiently amplified in the choroid plexus and liver, tissues known to synthesise TTR (Fig.…”

“…10 PCR analysis of reverse-transcribed RNA from various adult bovine tissues. The expected 121-bp PCR product was amplified with bovine TTR primers (Larsen and Kirkpatrick 1996) with 1 μg total RNA as template. Lane 1 DNA size markers (HindIII-digested λ-DNA), lane 2 fresh SCO, lane 3 organ-cultured SCO, lane 4 choroid plexus, lane 5 liver, lane 6 brain cortex.…”

“…For this purpose, RT-PCR with primers for bovine TTR (Larsen and Kirkpatrick 1996) and RNA samples from fresh and organ-cultured bovine SCO and control tissues, such as bovine choroid plexus, liver (Soprano et al 1985) and brain cortex, was performed. TTR mRNA was present in all samples but that of the brain cortex.…”

New insights into porcine-human synteny conservation

“…Six of these genes were from regions not painted with porcine chromosomal probes (ADCYAP1, ANT1, FGF7, GRIA2, GRIA3, and KLK3), while the rest were expected to confirm the general relationships defined by painting (Goureau et al 1996). The ADCYAP1 and ANT1 genes were initially amplified with published primers (Larsen et al 1996;Li and Womack 1997). For the other genes, PCR primers were designed from mammalian sequences available in Genbank.…”

Report of the Fourth International Workshop on Human Chromosome 18 Mapping 1996

“…Larsen et al (1996) used PCR and a panel of bovinc/rodent hybrid cell lines to map the bovine orthologs of TYMS. ADCYAP1.…”