Seven-hour stabilization of 99Tcm-exametazime (HMPAO) for cerebral perfusion

Solanki, Chandra K.; Ep, Wraight; Rw, Barber; Cb, Sampson

doi:10.1097/00006231-199806000-00009

Cited by 5 publications

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“…The percents of primary lipophilic complex, hydrophilic complex and Tc‐99m free pertechnetate were determined at 0, 0.5, 1, 1.5 and 2 h after labelling by HPLC according to Solanki with 20 µl of sample in a Novapack C18 4 µm, 3.9 × 150 mm column (Waters, Milford Massachusetts, USA) in a gradient chromatograph system with on‐line sodium iodide radioactivity detector (FC‐3600, Bioscan, Washington DC, USA). Reverse phase column had never been exposed to samples containing stannous ion…”

Section: Methodsmentioning

confidence: 99%

“…Many attempts have been made to improve the utilization of labelled product, some have been aimed at fractionating a reconstituted vial, others have been sought to overcome the restrictions on eluate, but most of the works were focused on stretching the shelf life by addition of chelating agents, oxidizing agents, free radical scavengers or pH buffers . Favourable results have been achieved with cobalt chloride, resulting in a new commercial kit with 6 h of stability but not indicated for cell labelling…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4]6,7 Many attempts have been made to improve the utilization of labelled product, some have been aimed at fractionating a reconstituted vial, 6,8,9 others have been sought to overcome the restrictions on eluate, 3 but most of the works were focused on stretching the shelf life by addition of chelating agents, oxidizing agents, free radical scavengers or pH buffers. 3,[10][11][12][13][14][15] Favourable results have been achieved with cobalt chloride, resulting in a new commercial kit with 6 h of stability but not indicated for cell labelling. 4,16 It has been reported that HMPAO is more sensitive to radiolysis than other 99m Tc radiopharmaceuticals, and it can be inferred that self irradiation plays an important role in the molecule stability, because of the free radicals produced from the decay of 99m Tc.…”

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confidence: 99%

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Influence of radiolytic‐produced hydrogen peroxide on the stability of commercial kit 99mtechnetium‐exametazime preparation

Martínez

Miñana

Martín-Falquina

et al. 2013

Labelled Comp Radiopharmac

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99mTc-d,l-hexamethylpropylene amine oxime ((99m) Tc-d,l-HMPAO) is a widely used radiopharmaceutical that suffers from an inherent instability with a shelf life of 30 min that constrains its availability for clinical use. A protocol for improving the stability of the kit with minimal modification of manufacturer's instructions and no chemicals addition to the commercial formulation is proposed. The protocol is based on the displacement of the oxygen present in the preparation, preventing free radicals build up and free pertechnetate formation. Although the degradation of (99m) Tc-d,l-HMPAO cannot be explained solely by the radiolytic production of free radicals, it appears to be an important factor in the shelf stability of the complex.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Influence of radiolytic‐produced hydrogen peroxide on the stability of commercial kit 99mtechnetium‐exametazime preparation

Martínez

Miñana

Martín-Falquina

et al. 2013

Labelled Comp Radiopharmac

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“…Para o fracionamento do Ceretec ® em até cinco doses, por exemplo, é recomendado o uso de solução fisiológica purgada com nitrogênio e, antes da marcação, a adição extra de Sn 2+ utilizando solução de um "kit" de pirofosfato reconstituído sem a adição do radioisótopo (58) . Outra alternativa para este radiofármaco fracionado é a adição extra de Sn 2+ , mais uma mistura de ácido gentísico e etanol, o que permite utilizar o produto por até sete horas após a marcação (59) . Para o "kit" de Neurolite ® , cujo frasco contendo o produto liofilizado é reconstituído utilizando-se 3 ml de solução fisiológica, dos quais é utilizado apenas 1 ml para o processo de marcação, os 2 ml restantes podem ser fracionados em volumes de 0,5 ml e congelados por até quatro semanas (60) .…”

Section: Os Procedimentos De Marcaçãounclassified

Alguns aspectos sobre geradores e radiofármacos de tecnécio-99m e seus controles de qualidade

2001

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Radiofármacos marcados com tecnécio-99m são os principais agentes para diagnósticos utilizados nas clínicas de medicina nuclear, em função de uma série de características físicas do radionuclídeo e pela praticidade dos radiofármacos serem preparados no local de uso, por meio de uma reação de complexação entre um agente complexante (fármaco) e o tecnécio-99m. Entretanto, durante esta reação podem ser geradas algumas impurezas que proporcionam a formação de produtos com baixa qualidade ou com características diferentes das desejadas. No presente trabalho serão apresentados alguns dos fatores que podem interferir na qualidade dos radiofármacos e os controles que podem ser utilizados para garantir sua qualidade.

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“…There are different methods of stabilizing 99m Tc-D,L-HMPAO: addition of gentisic acid (8), preparation of the radiopharmaceutical in ethanol (9,10), and addition of methylene blue (11) or cobalt chloride (11)(12)(13)(14). Of these methods, the addition of cobalt chloride after labeling has shown the best results.…”

mentioning

confidence: 99%

Stability of Stabilized 99mTc-D,L-HMPAO Stored in Vials and Syringes

Plancha-Mansanet

Caballero-Calabuig²,

Félix-Fontestad³

et al. 2008

Journal of Nuclear Medicine Technology

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Our objective was to determine the stability of stabilized 99m Tchexamethylpropylene amine oxime ( 99m Tc-D,L-HMPAO) dispensed by vial and syringe, with the storage time and labeling activity varied. Methods: 99m Tc-D,L-HMPAO was labeled according to the manufacturer's instructions, but with modification of the 99m TcO 4 Na activity. Two groups were prepared: 1,110 MBq (30 mCi) and 2,600-3,700 MBq (70.3-100 mCi). Five minutes after labeling, the radiochemical purity (RCP) of the vial content was determined. Afterward, the same activity was distributed into two 2-mL syringes and into the manufacturer's vial. In one of the syringes, the radiopharmaceutical stayed in contact with the needle for 4 h. At 2 and 4 h after labeling, the RCP of the vial and syringe content was checked and compared. Results: The mean RCP of stabilized 99m Tc-D,L-HMPAO labeled with 1,110 MBq (30 mCi) and stored in a vial decreased from 93.1% at 5 min to 92.1% at 2 h and to 91.1% at 4 h. With storage in a syringe, the RCP decreased from 89.8% at 2 h to 88.7% at 4 h. This diminution increased for labeling with higher activities (2,600-3,700 MBq [70.3-100 mCi]), ranging from 91.4% at 5 min, 89.0% at 2 h, and 85.3% at 4 h in a vial and from 85.9% at 2 h to 80.2% in a syringe. 99m TcO 2 and secondary 99m Tc-HMPAO were the main impurities at t 5 0. 99m TcO 4 2 was an impurity that increased with time in both vials and syringes but significantly so in syringes. All these impurities were higher with labeling activities in the range of 2,600-3,700 MBq (70.3-100 mCi). Contact of the needle with 99m Tc-D,L-HMPAO sharply decreased the RCP to 57.1% at 4 h. Conclusion: The RCP of stabilized 99m Tc-D,L-HMPAO decreases significantly in both vials and syringes with high labeling activities. The product is less stable when stored in a syringe than in a vial. The fraction of dose in contact with the needle affects the RCP results.

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Seven-hour stabilization of 99Tcm-exametazime (HMPAO) for cerebral perfusion

Cited by 5 publications

References 0 publications

Influence of radiolytic‐produced hydrogen peroxide on the stability of commercial kit 99mtechnetium‐exametazime preparation

Influence of radiolytic‐produced hydrogen peroxide on the stability of commercial kit 99mtechnetium‐exametazime preparation

Alguns aspectos sobre geradores e radiofármacos de tecnécio-99m e seus controles de qualidade

Stability of Stabilized 99mTc-D,L-HMPAO Stored in Vials and Syringes

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