Several Tick-Borne Pathogenic Viruses in Circulation in Anatolia, Turkey

Dinçer, Ender; Timurkan, Mehmet Ozkan; Oğuz, Bekir; Sahindokuyucu, Ismail; Şahan, Adem; Ekinci, Mustafa; Polat, Ceylan; Ergünay, Koray

doi:10.1089/vbz.2021.0082

Cited by 10 publications

(7 citation statements)

References 49 publications

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“…Therefore, gTJ-seg2 assay was used to screen ticks, belonging to eight species, collected in Corsica, and successfully detected JMTV in 22 pools of R. bursa, H. marginatum and R. sanguineus, with the highest JMTV detection rate found in R. bursa. JMTV has previously been detected in these tick species from other regions: R. bursa from Türkiye and Romania, in H. marginatum from Türkiye and in R. sanguineus from Türkiye and China 1,10,11,32 . The varying tick numbers and different detection methods prevent direct comparisons with these studies.…”

Section: Discussionmentioning

confidence: 74%

First detection of Jingmen tick virus in Corsica, France and development of a real time detection system for multiple tick-associated jingmenviruses

Cicculli,

Colmant,

Piorkowski

et al. 2024

Preprint

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Jingmen tick virus (JMTV) is a recently discovered segmented RNA virus, closely related to flaviviruses. It was identified for the first time in 2014, in China and subsequently in Brazil. Following this discovery, JMTV-related sequences have been identified in arthropods, vertebrates (including humans), plants, fungus and environmental samples from Asia, America, Africa, Europe and Oceania. Several studies suggest an association between these segmented flavi-like viruses, termed jingmenviruses, and febrile illness in humans. The development of rapid diagnostic assays for these viruses is therefore crucial to be prepared for a potential epidemic, for the early detection of these viruses via vector surveillance or hospital diagnosis. In this study, we designed a RT-qPCR assay to detect tick-associated jingmenviruses, validated it and tested its range and limit of detection with six tick-associated jingmenviruses using in vitro transcripts. Then we screened ticks collected in Corsica (France) from different livestock species, in order to determine the distribution of these viruses on the island. In total, 6,269 ticks from eight species were collected from 763 cattle, 538 horses, 106 sheep and 218 wild boars and grouped in 1,715 pools. We report the first detection of JMTV in Corsica, in Rhipicephalus bursa, Hyalomma marginatum and R. sanguineus ticks collected from cattle and sheep. The highest prevalence was found in the Rhipicephalus genus. The complete genome of a Corsican JMTV was obtained from a pool of Rhipicephalus bursa ticks and shares between 94.7% and 95.1% nucleotide identity with a JMTV sequence corresponding to a human patient in Kosovo and groups phylogenetically with European JMTV strains. These results show that a Mediterranean island such as Corsica could act as a sentinel zone for future epidemics.

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Section: Discussionmentioning

confidence: 74%

First detection of Jingmen tick virus in Corsica, France and development of a real time detection system for multiple tick-associated jingmenviruses

Cicculli,

Colmant,

Piorkowski

et al. 2024

Preprint

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“…There was some evidence that humans and several ticks could be infected with TcTV-2. It also appeared in regions outside of China including Turkey and Kazakhstan ( Li et al, 2015 ; Dong et al, 2021 ; Dincer et al, 2022 ; Jia et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Diversity of species and geographic distribution of tick-borne viruses in China

Wu,

Zhou,

Mao

et al. 2024

Front. Microbiol.

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IntroductionTick-borne pathogens especially viruses are continuously appearing worldwide, which have caused severe public health threats. Understanding the species, distribution and epidemiological trends of tick-borne viruses (TBVs) is essential for disease surveillance and control.MethodsIn this study, the data on TBVs and the distribution of ticks in China were collected from databases and literature. The geographic distribution of TBVs in China was mapped based on geographic locations of viruses where they were prevalent or they were detected in vector ticks. TBVs sequences were collected from The National Center for Biotechnology Information and used to structure the phylogenetic tree.ResultsEighteen TBVs from eight genera of five families were prevalent in China. Five genera of ticks played an important role in the transmission of TBVs in China. According to phylogenetic analysis, some new viral genotypes, such as the Dabieshan tick virus (DTV) strain detected in Liaoning Province and the JMTV strain detected in Heilongjiang Province existed in China.DiscussionTBVs were widely distributed but the specific ranges of viruses from different families still varied in China. Seven TBVs belonging to the genus Orthonairovirus of the family Nairoviridae such as Nairobi sheep disease virus (NSDV) clustered in the Xinjiang Uygur Autonomous Region (XUAR) and northeastern areas of China. All viruses of the family Phenuiviridae except Severe fever with thrombocytopenia syndrome virus (SFTSV) were novel viruses that appeared in the last few years, such as Guertu virus (GTV) and Tacheng tick virus 2 (TcTV-2). They were mainly distributed in the central plains of China. Jingmen tick virus (JMTV) was distributed in at least fourteen provinces and had been detected in more than ten species of tick such as Rhipicephalus microplus and Haemaphysalis longicornis, which had the widest distribution and the largest number of vector ticks among all TBVs. Parainfluenza virus 5 (PIV5) and Lymphatic choriomeningitis virus (LCMV) were two potential TBVs in Northeast China that could cause serious diseases in humans or animals. Ixodes persulcatus carried the highest number of TBVs, followed by Dermacentor nuttalli and H. longicornis. They could carry as many as ten TBVs. Three strains of Tick-borne encephalitis (TBEV) from Inner Mongolia Province clustered with ones from Russia, Japan and Heilongjiang Province, respectively. Several SFTSV strains from Zhejiang Province clustered with strains from Korea and Japan. Specific surveillance of dominant TBVs should be established in different areas in China.

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“…The study cohort comprised 40 tick pools, collected in Anatolia, Turkey during 2020–2021 ( Supplementary Table 1 ) (Dinçer et al, 2022 ). Individual adult ticks had been collected from infested animals including cattle ( Bos taurus ), sheep ( Ovis aries ), and dogs ( Canis familiaris ).…”

Section: Methodsmentioning

confidence: 99%

Impact of nanopore-based metagenome sequencing on tick-borne virus detection

Ergunay,

Dincer,

Justi

et al. 2023

Front. Microbiol.

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IntroductionWe evaluated metagenomic nanopore sequencing (NS) in field-collected ticks and compared findings from amplification-based assays.MethodsForty tick pools collected in Anatolia, Turkey and screened by broad-range or nested polymerase chain reaction (PCR) for Crimean-Congo Hemorrhagic Fever Virus (CCHFV) and Jingmen tick virus (JMTV) were subjected to NS using a standard, cDNA-based metagenome approach.ResultsEleven viruses from seven genera/species were identified. Miviruses Bole tick virus 3 and Xinjiang mivirus 1 were detected in 82.5 and 2.5% of the pools, respectively. Tick phleboviruses were present in 60% of the pools, with four distinct viral variants. JMTV was identified in 60% of the pools, where only 22.5% were PCR-positive. CCHFV sequences characterized as Aigai virus were detected in 50%, where only 15% were detected by PCR. NS produced a statistically significant increase in detection of these viruses. No correlation of total virus, specific virus, or targeted segment read counts was observed between PCR-positive and PCR-negative samples. NS further enabled the initial description of Quaranjavirus sequences in ticks, where human and avian pathogenicity of particular isolates had been previously documented.DiscussionNS was observed to surpass broad-range and nested amplification in detection and to generate sufficient genome-wide data for investigating virus diversity. It can be employed for monitoring pathogens in tick vectors or human/animal clinical samples in hot-spot regions for examining zoonotic spillover.

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Several Tick-Borne Pathogenic Viruses in Circulation in Anatolia, Turkey

Cited by 10 publications

References 49 publications

First detection of Jingmen tick virus in Corsica, France and development of a real time detection system for multiple tick-associated jingmenviruses

First detection of Jingmen tick virus in Corsica, France and development of a real time detection system for multiple tick-associated jingmenviruses

Diversity of species and geographic distribution of tick-borne viruses in China

Impact of nanopore-based metagenome sequencing on tick-borne virus detection

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