Severe childhood asthma and allergy to furry animals: Refined assessment using molecular‐based allergy diagnostics

Konradsen, Jon R.; Nordlund, Björn; Önell, Annica; Borres, Magnus P.; Grönlund, Hans; Hedlin, Gunilla

doi:10.1111/pai.12198

Cited by 96 publications

(121 citation statements)

References 28 publications

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“…Fel d 4-specific IgEs were found in 39% of serum samples from cat-allergic patients. This results are in a well agreement with 34% of Fel d4 positive samples obtained in 2014 years study (Konradsen et al 2014) and differ from another study in which the percentage of patients sensitized to Fel d 4 reached 63% (Smith et al 2004). Differences in IgE binding could be caused by various factors.…”

Section: Discussionsupporting

confidence: 70%

High-fidelity PCR enzyme with DNA-binding domain facilitates de novo gene synthesis

Dolgova

Стуколова

2017

3 Biotech

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Nowadays enzymatic synthesis of genes is the most powerful tool for fast resolution of the various tasks in the field of basic and applied biological research. PCRbased gene assembly from overlapping oligonucleotides has become a widely used strategy. However, all the methods described in the literature are not perfect and need an extra processing step. In this study we are verifying Phusion high-fidelity polymerase as a tool to reduce nucleotide mismatches in de novo gene synthesis, thus facilitating subsequent cloning. To test the efficiency of the polymerase, we selected Fel d 4 gene, which is a 581 bp DNA sequence encoding the lipocalin allergen protein, one of the major cat allergens. The approach described here, therefore, would be useful in DNA sequences creation.

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Section: Discussionsupporting

confidence: 70%

High-fidelity PCR enzyme with DNA-binding domain facilitates de novo gene synthesis

Dolgova

Стуколова

2017

3 Biotech

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“…Specific IgE to Can f 2 was significantly associated with asthma diagnosis, Can f 3 with moderate/severe rhinitis and asthma diagnosis, Can f 5 with persistent and moderate-severe rhinitis, and Equ c 3 with persistent rhinitis and severe asthma [64]. Furthermore, the sensitization to Can f 2 and Equ c 1 was more common in severe asthma than in children with controlled asthma [65]. Asthmatic children with cat allergy have higher Fel d 1-specific IgE levels as compared to those with rhinitis alone, and this suggests that high IgE levels to Fel d 1 could be a marker of increased asthma risk [66].…”

Section: Animal Dander Sensitization and Severe Asthmamentioning

confidence: 99%

Is allergic sensitization relevant in severe asthma? Which allergens may be culprits?

Lombardi

Savi

Ridolo

et al. 2017

World Allergy Organization Journal

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Severe asthma is a major health concern. The allergic (IgE-mediated) form of asthma is well known from a pathogenic viewpoint. We searched the available literature to identify which allergens are most frequently associated with severe, refractory or life threatening asthma. According to the results, molds, pet dander, cockroach and ragweed were more frequently responsible for severe asthma. Thunderstorm asthma, in addition, represents a special association between allergic sensitization and an external climatic factor. A detailed knowledge of the most harmful allergens is mandatory for an appropriate diagnostic and preventive approach.

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“…It has recently been reported that CRD may improve the assessment of asthma (31,43) and help better understanding the role of allergy in severe asthma in childhood (44). However, it is likely that better interpretation algorithms are needed to capitalize fully on the potential of this exciting new technology (43).…”

Section: Component-resolved Diagnostics In Asthmamentioning

confidence: 99%

Allergy in severe asthma

Giacco

Bakırtaş

Bel

et al. 2016

Allergy

116

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It is well recognized that atopic sensitization is an important risk factor for asthma, both in adults and in children. However, the role of allergy in severe asthma is still under debate. The term 'Severe Asthma' encompasses a highly heterogeneous group of patients who require treatment on steps 4-5 of GINA guidelines to prevent their asthma from becoming 'uncontrolled', or whose disease remains 'uncontrolled' despite this therapy. Epidemiological studies on emergency room visits and hospital admissions for asthma suggest the important role of allergy in asthma exacerbations. In addition, allergic asthma in childhood is often associated with severe asthma in adulthood. A strong association exists between asthma exacerbations and respiratory viral infections, and interaction between viruses and allergy further increases the risk of asthma exacerbations. Furthermore, fungal allergy has been shown to play an important role in severe asthma. Other contributing factors include smoking, pollution and work-related exposures. The 'Allergy and Asthma Severity' EAACI Task Force examined the current evidence and produced this position document on the role of allergy in severe asthma.

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Severe childhood asthma and allergy to furry animals: Refined assessment using molecular‐based allergy diagnostics

Abstract: Children with severe allergic asthma had higher sIgE levels to cat, dog and horse. Molecular-based allergy diagnostics revealed a more complex molecular spreading of allergen components in children with the most severe disease.

Cited by 96 publications

References 28 publications

High-fidelity PCR enzyme with DNA-binding domain facilitates de novo gene synthesis

High-fidelity PCR enzyme with DNA-binding domain facilitates de novo gene synthesis

Is allergic sensitization relevant in severe asthma? Which allergens may be culprits?

Allergy in severe asthma

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