Severe deoxyribonucleic acid damage after out-of-hospital cardiac arrest in successfully resuscitated humans

Hazuková, Radka; Řezáčová, Martina; Kočí, Jaromír; Čermáková, Eva; Pleskot, M

doi:10.1016/j.ijcard.2016.01.046

Cited by 4 publications

(10 citation statements)

References 10 publications

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“…We did not find apparent differences in DNA damage between cardiac and non-cardiac etiology of OHCA or between surviving and non-surviving patients using either gH2AX (2) or the comet assay method (this study). In contrast to gH2AX, which is an indirect marker of DNA damage (gH2AX positivity reflects the intensive DNA reparation) (2), the comet assay directly displays DNA damage.…”

Section: Discussioncontrasting

confidence: 64%

“…Despite the existence of several papers on DNA damage in cardiac disease (11–20), currently, very few papers have been published on genomic markers during and after OHCA (2, 21–24). White et al (21) tested DNA from the cerebral cortex of dogs during their reperfusion following resuscitation for cardiac arrest, but no significant damage was found.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, we detected DNA damage (using gH2AX method) at admission in a majority of successfully resuscitated patients from OHCA (83%) (2). Similar results were found in this work (using comet assay).…”

Section: Discussionmentioning

confidence: 99%

“…Inclusion and exclusion criteria have been previously reported (2). The patients enrolled in the study were all consecutive adults (age ≥18 years) who were successfully resuscitated [return of spontaneous circulation within 30 min, survival for ≥60 min following arrival at the emergency department (ED)] by professionals from non-traumatic OHCA of either cardiac or non-cardiac etiology (Table 1) and who met none of the exclusion criteria [active malignancy, the terminal phase of a chronic illness, toxic or suicidal causes (including drowning cases), chemotherapy or radiotherapy within the last year, and X-ray investigation within the last month or before the blood for DNA analysis was sampled).…”

Section: Methodsmentioning

confidence: 99%

“…Prognostication is now based on a multimodal algorithm applied at ≥48 and ≥120 h after OHCA (1). However, the role of DNA integrity in prognostication of patients after OHCA remains unknown (2). …”

Section: Introductionmentioning

confidence: 99%

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Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest

Hazuková

Řezáčová

Köhlerová³

et al. 2017

Anatol J Cardiol

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Objective:This study aimed to investigate whether out-of-hospital cardiac arrest (OHCA) may induce severe DNA damage measured using comet assay in successfully resuscitated humans and to evaluate a short-term prognostic role.Methods:In this prospective, controlled, blinded study (1/2013–1/2014), 41 patients (age, 63±14 years) successfully resuscitated from non-traumatic OHCA and 10 healthy controls (age, 53±17 years) were enrolled. DNA damage [double-strand breaks (DSBs) and single-strand breaks (SSBs)] was measured using comet assay in peripheral lymphocytes sampled at admission. Clinical data were recorded (according to Utstein style). A good short-term prognosis was defined as survival for 30 days.Results:Among the patients, there were 71% (29/41) short-term survivors. After OHCA, DNA damage (DSBs and SSBs) was higher (11.0±7.6% and 0.79±2.41% in tail) among patients than among controls (1.96±1.63% and 0.02±0.03% in tail), and it was more apparent for DSBs (p<0.001 and p=0.085). There was no difference in the DNA damage between patients with cardiac and non-cardiac etiology, or between survivors and nonsurvivors. Among Utstein style parameters, ventricular fibrillation, asystole, and early electrical defibrillation influenced DSB; none of the factors influenced SSBs. Factors influencing survival were SSBs, ventricular fibrillation, length of cardiopulmonary resuscitation by professionals ≤15 min, cardiogenic shock, and postanoxic encephalopathy. In contrast to DSBs [area under the curve (AUC)=0.520], SSBs seem to have a potential in prognostication (AUC=0.639).Conclusion:This study for the first time demonstrates revelation of DNA damage using comet assay in patients successfully resuscitated from OHCA. Whether DNA damage measured using comet assay may be a prognostic marker remains unknown, although our data may encourage some suggestions.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest

Hazuková

Řezáčová

Köhlerová³

et al. 2017

Anatol J Cardiol

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Arterial hypertension and significant DNA damage - from cell lines to patients

Hazuková

2024

Cor Vasa

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Oxidative DNA Damage and Arterial Hypertension in Light of Current ESC Guidelines

Hazuková,

Zadák,

Pleskot

et al. 2024

IJMS

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A new insight into oxidative stress is based on oxidative deoxyribonucleic acid (DNA) damage. DNA is the pivotal biopolymer for life and health. Arterial hypertension (HT) is a globally common disease and a major risk factor for numerous cardiovascular (CV) conditions and non-cardiac complications, making it a significant health and socio-economic problem. The aetiology of HT is multifactorial. Oxidative stress is the main driver. Oxidative DNA damage (oxidised guanosine (8OHdG), strand breaks (SSBs, DSBs)) seems to be the crucial and initiating causal molecular mechanism leading to HT, acting through oxidative stress and the resulting consequences (inflammation, fibrosis, vascular remodelling, stiffness, thickness, and endothelial dysfunction). In light of the current European Society of Cardiology (ESC) guidelines with defined gaps in the evidence, this manuscript, for the first time, (1) summarizes evidence for oxidative DNA damage in HT and other CV risk factors, (2) incorporates them into the context of known mechanisms in HT genesis, (3) proposes the existing concept of HT genesis innovatively supplemented with oxidative DNA damage, and (4) mentions consequences such as promising new targets for the treatment of HT (DNA damage response (DDR) pathways).

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Severe deoxyribonucleic acid damage after out-of-hospital cardiac arrest in successfully resuscitated humans

Cited by 4 publications

References 10 publications

Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest

Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest

Arterial hypertension and significant DNA damage - from cell lines to patients

Oxidative DNA Damage and Arterial Hypertension in Light of Current ESC Guidelines

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