Severe fever with thrombocytopenia syndrome, an emerging tick-borne zoonosis

Liu, Quan; He, Bingfang; Huang, Si-Yang; Wei, Feng; Zhu, Xingquan

doi:10.1016/s1473-3099(14)70718-2

Cited by 456 publications

(422 citation statements)

References 106 publications

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“…(Since the initial report, the current case fatality in China is estimated at 2 to 15% [11,12]). The disease was originally suspected to be anaplasmosis, but some clinical signs and symp- …”

Section: Importancementioning

confidence: 99%

Reverse Genetics System for Severe Fever with Thrombocytopenia Syndrome Virus

Brennan

Zhang

et al. 2015

J Virol

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Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne pathogen that was first reported in China in 2009. Phylogenetic analysis of the viral genome showed that SFTS virus represents a new lineage within the Phlebovirus genus, distinct from the existing sandfly fever and Uukuniemi virus groups, in the family Bunyaviridae. SFTS disease is characterized by gastrointestinal symptoms, chills, joint pain, myalgia, thrombocytopenia, leukocytopenia, and some hemorrhagic manifestations with a case fatality rate of about 2 to 15%. Here we report the development of reverse genetics systems to study STFSV replication and pathogenesis. We developed and optimized functional T7 polymerase-based M-and S-segment minigenome assays, which revealed errors in the published terminal sequences of the S segment of the Hubei 29 strain of SFTSV. We then generated recombinant viruses from cloned cDNAs prepared to the antigenomic RNAs both of the minimally passaged virus (HB29) and of a cell culture-adapted strain designated HB29pp. The growth properties, pattern of viral protein synthesis, and subcellular localization of viral N and NSs proteins of wild-type HB29pp (wtHB29pp) and recombinant HB29pp viruses were indistinguishable. We also show that the viruses fail to shut off host cell polypeptide production. The robust reverse genetics system described will be a valuable tool for the design of therapeutics and the development of killed and attenuated vaccines against this important emerging pathogen. IMPORTANCESFTSV and related tick-borne phleboviruses such as Heartland virus are emerging viruses shown to cause severe disease in humans in the Far East and the United States, respectively. Study of these novel pathogens would be facilitated by technology to manipulate these viruses in a laboratory setting using reverse genetics. Here, we report the generation of infectious SFTSV from cDNA clones and demonstrate that the behavior of recombinant viruses is similar to that of the wild type. This advance will allow for further dissection of the roles of each of the viral proteins in the context of virus infection, as well as help in the development of antiviral drugs and protective vaccines.T he family Bunyaviridae is one of the largest taxonomic groupings of RNA viruses, containing over 350 named isolates that are classified into five genera: Hantavirus, Nairovirus, Orthobunyavirus, Phlebovirus, and Tospovirus (1). All viruses share a genome structure that comprises three segments of negative-sense or ambisense RNA, named small (S), medium (M), and large (L). The S segment encodes the nucleocapsid (N) protein; the M segment encodes the virion glycoproteins Gn and Gc; and the L segment encodes the L protein, the viral RNA-dependent RNA polymerase. Some viruses also encode nonstructural proteins on the S and M segments. Viruses that impinge on human health, either directly by causing human disease or indirectly by causing economic losses of domestic animals or crop plants, are found in each of the five gener...

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Section: Importancementioning

confidence: 99%

Reverse Genetics System for Severe Fever with Thrombocytopenia Syndrome Virus

Brennan

Zhang

et al. 2015

J Virol

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“…İlk kez Çin'in kırsal yörelerinde tanımlanan daha sonra bu ülkenin 11 ilinde yüksek mortalite oranıyla kendini gösteren SFTS daha sonra Japonya ve Kore'de gözlenmiş ve artık dünya-nın başka yerlerinde de halk sağlığı yönünden önemli bir risk hâline gelmiştir (22) .…”

Section: Esas Itibariyle Bahar Ve Yaz Aylarındaunclassified

An overview of tick-borne viral pathogens in the light of current literature

Aktaş¹,

Aydın²

2017

TMCD

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An Overview of Tick-Borne Viral Pathogens in the Light of Current Literature Keywords: Tick-borne viruses, Heartland virus, CrimeanCongo hemorrhagic fever GiRiŞKenelere ilişkin ilk yazılara kene humması hakkında MÖ 1550 tarihli Mısır papirüslerinde rastlandığı, yazarı bilinen ilk yazıların ise MÖ 800 yılında Homeros tarafından yazıldığı sanılmaktadır (1) . Hastalıklarla ilişkisi yüzyıllar öncesinden bilinen keneler, insan ve hayvanlarda ölümle sonuçlanabilen çeşitli enfeksiyon etkenlerini taşımaları ile günümüze önemini arttırarak gelmişlerdir.Karasal omurgalı canlı gruplarının tüm sınıfları-nın zorunlu dış paraziti olan kenelerin tek besin

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“…Globally, the most important genera of pathogenic tick-borne bacteria with arboterrorism potential are Borrelia, Rickettsia, Ehrlichia and Francisella. Amongst the most important tick-borne viruses are the Crimean-Congo hemorrhagic fever virus, Colorado tick fever virus and the SFTS virus [19,20]. The protozoan genera Babesia and Theileria are also spread by ticks and are of great concern [19].…”

Section: Ticks As Arboweaponsmentioning

confidence: 99%

Arboterrorism: Doubtful Delusion or Deadly Danger

Kwak¹

2017

J Bioterror Biodef

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Arboterrorism, the weaponisation and use of arthropod vectors carrying pathogenic microbes, is a worrying possibility in an age of rapidly accelerating biology. Historically, arboterrorism has attracted minimal research and the probability of such attacks occurring has been unclear. For the first time the logistics of such attacks are discussed along with potential vectors and pathogens. A novel biodefence strategy against arboterrorism is also proposed.

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Severe fever with thrombocytopenia syndrome, an emerging tick-borne zoonosis

Cited by 456 publications

References 106 publications

Reverse Genetics System for Severe Fever with Thrombocytopenia Syndrome Virus

Reverse Genetics System for Severe Fever with Thrombocytopenia Syndrome Virus

An overview of tick-borne viral pathogens in the light of current literature

Arboterrorism: Doubtful Delusion or Deadly Danger

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