Due to an increasing trend towards environmentally friendly public transport, rail networks face higher speeds and wheel loads affecting the material degradation of the railway components. Within this study, influencing parameters on the formation of stratified surface layers, forming on railway wheels during service due to thermal loadings in the wheel-rail contact, are studied. These layers consist of white etching layer and underlying brown etching layer and are susceptible to fatigue crack initiation. By using laser surface treatments, its formation based on two consecutive thermal loadings is systematically demonstrated on ER7 wheel steel. Further, a decrease in layer thickness with decreasing laser power, and an increase in brown etching layer thickness with increasing laser power difference is shown. Moreover, the effect of finer grain size leading to an increased layer thickness, and the influence of the chemical composition by comparing the standard ER7 wheel steel to a micro-alloyed wheel steel are demonstrated.