Introduction: There is a paucity of studies investigating how sexual activity is perceived to influence sleep, despite conceptions about significant gender differences regarding this issue. Methods: In all, 4000 persons, aged between 18 and 55 years, were randomly drawn from the Norwegian Population Registry and invited to participate in a postal survey. The respondents were asked how sexual activity with another person, with or without orgasm, and how masturbation, with and without orgasm, influenced sleep latency and sleep quality. Results: A total of 1080 persons participated (response rate 28.2%) of which 56.1% were women. The mean age of the sample was 38.7 years (SD = 10.8). Sexual activity with an orgasm was perceived to have a soporific effect by both men and women. Sexual activity with another person, with an orgasm, was perceived to have a relatively stronger effect on men compared to women in terms of sleep quality. Sexual activity without an orgasm was by men reported to have a sleep impairing effect, whereas the perceived effect reported by women was equivocal. Conclusions: Sexual activity with orgasms was perceived as having a soporific effect in both men and women. Sexual activity without an orgasm had an equivocal perceived effect on sleep.