Sex-dependent composition of cuticular hydrocarbons of the colorado beetle, Leptinotarsa decemlineata say

Dubis, E.; Maliński, Edmund; Dubis, Alina T.; Szafranek, Janusz; Nawrot, J.; PopŁlawski, Janusz; Wróbel, Jerzy T.

doi:10.1016/0300-9629(87)90002-8

Cited by 29 publications

(18 citation statements)

References 6 publications

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“…The relative composition of the alkanes was very similar in the four (2) wax esters; (3) benzoic acid esters, fatty acid methyl, ethyl, isopropyl and phenylethyl esters, aldehydes, and ketones; (4) methyl ketones and unknown; (5) unknown; (6) fatty acids, primary and secondary alcohols; (7) sterols. varieties, and resembled that obtained in earlier work (Dubis et al, 1987). The similar patterns found in these four varieties, cultivated under different conditions, suggest that they are characteristic of potato plants in general.…”

Section: Alkanessupporting

confidence: 83%

“…These analyses of the four potato varieties show that potato leaf cuticular waxes are more complex than previously thought (Sen, 1987;Dubis et al, 1987). Methyl ketones, alkan-2-ols, sterols, b-amyrin, benzoic acid esters and fatty acid methyl, ethyl, isopropyl and phenylethyl esters were found for the first time in potato leaf waxes.…”

Section: Estersmentioning

confidence: 90%

“…Only the main classes of waxes have been identified so far. Long chain n-alkanes and 2-and 3-methylalkanes in the C 25 -C 33 range have been identified in the leaf cuticular waxes of six potato varieties (Dubis et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

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Cuticular waxes from potato (Solanum tuberosum) leaves

Szafranek

Synak

2006

Phytochemistry

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Section: Alkanessupporting

confidence: 83%

Section: Estersmentioning

confidence: 90%

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Cuticular waxes from potato (Solanum tuberosum) leaves

Szafranek

Synak

2006

Phytochemistry

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“…The CHC profile of this species is a complex mixture of straight-chained and methyl-branched saturated and unsaturated hydrocarbons with a wide range of carbon backbones (C19 -C45). The high percentage of 2-methyl alkanes seems to be typical for chrysomelids, representing major compounds in the CHC profiles of nearly all leaf beetle species investigated (Dubis et al 1987;Nikolova et al 1999;Nelson et al 2002Nelson and Charlet 2003;Peterson et al 2007;Geiselhardt et al 2009). The large quantities of methyl-branched alkenes in P. cochleariae is striking.…”

Section: Discussionmentioning

confidence: 97%

The Role of Cuticular Hydrocarbons in Male Mating Behavior of the Mustard Leaf Beetle, Phaedon cochleariae (F.)

2009

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We investigated the role that cuticular hydrocarbons (CHC) play in sexual communication by the mustard leaf beetle, Phaedon cochleariae (Coleoptera: Chrysomelidae). In laboratory bioassays, male P. cochleariae attempted to copulate with living or freeze-killed females as often as with males. However, the duration of copulation with females was longer than with males. To elucidate the impact of CHC on this behavior, cuticular compounds of adults of both sexes were extracted with dichloromethane. Male mating attempts with glass beads treated with the dichloromethane extract were nearly as frequent as with living beetles. The dichloromethane extract was fractionated by silica gel chromatography, and the biological activity of the fractions was tested by applying them to glass beads. A non-polar hexane fraction significantly elicited mating behavior, whereas the polar methanol fraction did not, likely because it contained defensive compounds from exocrine glands located in the elytra and pronota. Interestingly, a mixture of both the non-polar and polar fraction tended to elicit more mating attempts than did the non-polar hexane fraction alone. Further fractionation of the significantly active hexane fraction by silver nitrate column chromatography revealed that saturated CHC elicited mating behavior, but the olefins did not. GC-MS analyses of dichloromethane cuticular extracts showed that the male and female CHC profiles were qualitatively identical, but differed in their relative composition. Canonical discriminant analysis showed that CHC profiles of males and females formed separate clusters. Nevertheless, the results of our bioassays demonstrated that male and female CHC did not elicit sex discriminative male behavior, but induced mating by males regardless of the sex of the partner.

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“…Epicuticular grease has been considered to play a fundamental role in limiting water loss (e.g. Ramsay, 1935;Neville, 1975) and serving as a behavioral cue for insects (Dubis et al, 1987;Espelie et al, 1991). Its thickness previously has been suggested to be at a molecular scale (Beament, 1955), varying from less than 1 nm to several microns (Hendricks and Hadley, 1983), or ranging from 0.1 μm to 1.0 μm (Locke, 1964).…”

Section: Discussionmentioning

confidence: 99%

An insect trap as habitat: cohesion-failure mechanism prevents adhesion ofPameridea roridulaebugs to the sticky surface of the plantRoridula gorgonias

Voigt¹,

Gorb²

2008

Journal of Experimental Biology

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SUMMARYThe glandular trichomes of the plant Roridula gorgonias release an extremely adhesive, visco-elastic, resinous secretion that traps a variety of insects, including those having a considerable body size. However, the specialized mutualistic mirid bug Pameridea roridulae lives and walks on this sticky plant surface without being trapped. We have sought to reveal the mechanism underlying the apparent non-sticky nature of the cuticle of this bug. In this study, we have visualized intact plant and insect surfaces using cryo-scanning electron microscopy and measured the adhesive properties of the plant secretion on different surfaces. We present a combination of structural and experimental results that suggest that a thick and cohesively weak film of an outermost, epicuticular greasy secretion acts as a ʻsloughing-offʼ layer, preventing the formation of contacts between the sticky plant secretion and the solid insect cuticle. In a comparative study of fresh cuticle fractures of flies representing a typical prey of R. gorgonias, a thin, fragmentary layer of epicuticular grease was revealed. These results indicate that, when trapping prey, the plant adhesive might form proper contact with solid islands of the insect cuticle that are free of epicuticular grease.

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Sex-dependent composition of cuticular hydrocarbons of the colorado beetle, Leptinotarsa decemlineata say

Cited by 29 publications

References 6 publications

Cuticular waxes from potato (Solanum tuberosum) leaves

Cuticular waxes from potato (Solanum tuberosum) leaves

The Role of Cuticular Hydrocarbons in Male Mating Behavior of the Mustard Leaf Beetle, Phaedon cochleariae (F.)

An insect trap as habitat: cohesion-failure mechanism prevents adhesion ofPameridea roridulaebugs to the sticky surface of the plantRoridula gorgonias

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