Sex determination in cucumber (Cucumis sativus) as a model system for molecular biology

Malepszy, S.; Niemirowicz-Szczytt, K.

doi:10.1016/0168-9452(91)90271-9

Cited by 134 publications

(106 citation statements)

References 23 publications

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“…Members of the second group initiate both sex organ types, but the inappropriate set is suppressed or aborted at a later stage of development. Such species include Silene latifolia (Grant et al, 1994) ; Asparagus officinalis (Lazarte & Palser, 1979 ;Bracale et al, 1991) ; Cucumis (Atsmon & Galun, 1960 ;Malepszy & Niemirowicz-Szczytt, 1991) ; Pistacia (Hormaza & Polito, 1996) ; Actinidia (McNeilage, 1991a,b) ; Zea (Cheng et al, 1983 ;Stevens et al, 1986) ; and Rumex acetosa (Ainsworth et al, 1995(Ainsworth et al, , 1998. This study has shown that H. lupulus falls into the first and more unusual group as the sexes show extreme divergence in their floral morphologies at a very early stage in the floral development pathway.…”

Section: mentioning

confidence: 99%

Sexual development and sex chromosomes in hop

et al. 2000

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The stages of floral development in staminate and pistillate plants of hop (Humulus lupulus) were defined using scanning electron microscopy and light microscopy. Vegetative meristems of male and female plants are morphologically indistinguishable. On transition to the reproductive phase, inflorescence apices reduce greatly in size and striking developmental sex differences become apparent. The first sex-specific differences occur extremely early in floral ontogeny. Both male and female plants initiate inflorescence meristems at each leaf node, each meristem being enclosed within a bract. Male secondary inflorescence meristems give rise to clusters of asynchronously developing flowers. Female inflorescence meristems produce flowers arranged in ' cones '. Each male floral meristem initiates a whorl of five sepal primordia, followed by an inner whorl of five stamen primordia. There is no sign of carpel development at any stage. In females, two carpel primordia are initiated, surrounded at their base by a vestigial perianth whorl. No stamen development is observed. Several monoecious lines carry bisexual flowers, either within cymose panicles or within the basal bracts of terminal female inflorescences. Bisexual flowers usually possess perianth, stamen and carpel whorls. The central whorls are often highly variable, and range from a pair of stigmas fused to a thin central filament to a well developed gynoecium. Chimaeric central whorls consisting of fused staminoid-carpelloid structures also occur. Sex differences in unisexual hop flowers are determined at an extremely early stage in ontogeny. The inappropriate set of sex organs is suppressed before it becomes visible or, more probably, it is not initiated at all. Genes directing the development of sex are likely to act at an extremely early stage, well in advance of floral organogenesis. The sex chromosomes of dioecious hop plants are described, as well as the chromosome constitutions of monoecious plants and those carrying bisexual flowers.

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Section: mentioning

confidence: 99%

Sexual development and sex chromosomes in hop

et al. 2000

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“…The distribution of male and female flowers along the plant axis varies from one plant to the next, giving rise to various sex types (Malepszy and Niemirowicz-Szczytt 1991). Monoecious plants produce male flowers at the base of the main stem, then they produce male and female flowers on the middle part, and finally female flowers are produced at the top.…”

Section: Introductionmentioning

confidence: 99%

Expression of Two 1-Aminocyclopropane-1-Carboxylate Synthase Genes, CS-ACSI and CS-ACS2, Correlated with Sex Phenotypes in Cucumber plants (Cucumis sativus L.).

Kamachi

Mizusawa²,

Matsuura³

et al. 2000

Plant Biotechnology

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Ethylene regulates the sex expression in cucumber plants (Cucumis sativus L.). We examined expression of two 1-aminocyclopropane-1-Carboxylate (ACC) synthase genes, CS-ACS1 and CS-ACS2, at the apices of isogenic gynoecious (FF) and monoecious (ff) cucumber lines. The transcripts of CS-ACS1 and CS-ACS2 were detected at the apices of gynoecious line. On the other hand, only the CS -ACS2 transcript was detected at the apices of monoecious line . The expression of CS-ACS2 at the apices was localized to the floral buds that would develop into female flowers. These results suggest that the differentiation of female flowers at the apices of isogenic gynoecious and monoecious cucumber plants is regulated by the levels of both CS-ACS1 and CS-ACS2 mRNA at the apices.

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“…The commonly accepted cucumber genome size is 367 Mkb/C and 1000 cM [1], and the karyotype consists of seven relatively small chromosomes [2]. Cucumber can be monoecious or dioecious, as determined by a simple configuration of four nonallelic cooperating main sex-determining genes [3]. The ongoing molecular tests on cucumber being conducted in various centres worldwide are mainly focused on the determination of the genome structure (including the nuclear and organelle genomes), and more recently on the determination of the transcriptome [1][2].…”

Section: Introductionmentioning

confidence: 99%

The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’

Gutman¹,

Pawełkowicz²,

Wóycicki³

et al. 2008

Cellular and Molecular Biology Letters

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Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene sequencing. A BAC genomic library was constructed from high molecular weight DNA (HMW DNA) obtained from nuclei of the cucumber (Cucumis sativus L. cv. Borszczagowski; B10 line). The DNA was digested with the HindIII restriction enzyme and ligated into the pCC1BAC vector. The library consists of 34,560 BAC clones with an average insert size of 135 kb, and 12.7x genome coverage. Screening the library for chloroplast and mitochondrial DNA content indicated an exceptionally low 0.26% contamination with chloroplast DNA and 0.3% with mitochondrial DNA.

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Sex determination in cucumber (Cucumis sativus) as a model system for molecular biology

Cited by 134 publications

References 23 publications

Sexual development and sex chromosomes in hop

Sexual development and sex chromosomes in hop

Expression of Two 1-Aminocyclopropane-1-Carboxylate Synthase Genes, CS-ACSI and CS-ACS2, Correlated with Sex Phenotypes in Cucumber plants (Cucumis sativus L.).

The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’

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