Sex Determination in Sturgeon

Wuertz, Sven; Güralp, Hilal; Pšenička, Martin; Chebanov, Mikhail S.

doi:10.1002/9781119127291.ch33

Cited by 7 publications

(8 citation statements)

References 112 publications

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“…It is also important to note that early gonadal development in A. mexicanus is not synchronous within the laboratory population we used. Such results were reported in different species of sturgeons showing asynchronous development during early gonadal differentiation [Wuertz et al, 2018].…”

Section: Discussionsupporting

confidence: 81%

Primordial Germ Cell Migration and Histological and Molecular Characterization of Gonadal Differentiation in Pachón Cavefish Astyanax mexicanus

Imarazene¹,

Beille²,

Jouanno³

et al. 2020

Sex Dev

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The genetic regulatory network governing vertebrate gonadal differentiation appears less conserved than previously thought. Here, we investigated the gonadal development of Astyanax mexicanus Pachón cavefish by looking at primordial germ cells (PGCs) migration and proliferation, gonad histology, and gene expression patterns. We showed that PGCs are first detected at the 80% epiboly stage and then reach the gonadal primordium at 1 day post-fertilization (dpf). However, in contrast to the generally described absence of PGCs proliferation during their migration phase, PGCs number in cavefish doubles between early neurula and 8–9 somites stages. Combining both gonadal histology and vasa (germ cell marker) expression patterns, we observed that ovarian and testicular differentiation occurs around 65 dpf in females and 90 dpf in males, respectively, with an important inter-individual variability. The expression patterns of dmrt1, gsdf, and amh revealed a conserved predominant male expression during cavefish gonadal development, but none of the ovarian differentiation genes, i. e., foxl2a, cyp19a1a, and wnt4b displayed an early sexually dimorphic expression, and surprisingly all these genes exhibited predominant expression in adult testes. Altogether, our results lay the foundation for further research on sex determination and differentiation in A. mexicanus and contribute to the emerging picture that the vertebrate sex differentiation downstream regulatory network is less conserved than previously thought, at least in teleost fishes.

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Section: Discussionsupporting

confidence: 81%

Primordial Germ Cell Migration and Histological and Molecular Characterization of Gonadal Differentiation in Pachón Cavefish Astyanax mexicanus

Imarazene¹,

Beille²,

Jouanno³

et al. 2020

Sex Dev

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“…that the female has been suggested as heterogametic (ZW) and males as homogametic sex (ZZ), due to the sex ratio of meiotic gynogens observed. The percentage of males ranged between 18%-50% in white sturgeon (Acipenser transmontanus Richardson, 1836) (Van Eenennaam et al, 1999), 20%-30% in hybrid bester (Omoto, Maebayashi, Adachi, Arai, & Yamauchi, 2005), 19% in Siberian sturgeon (Acipenser baerii Brandt, 1869) (Fopp-Bayat, 2010), 20% in paddlefish (Polyodon spathula Lacépède, 1797) (Shelton & Mims, 2012) and 28% in ship sturgeon (A. nudiventris Lovetsky, 1828) , confirming a common sex determination mechanism (but not a sex determining master gene) among Acipenseriformes (Wuertz et al, 2018).…”

mentioning

confidence: 87%

“…The sex in sturgeon is mainly genetically determined and there is no evidence for environmental sex determination (Doroshov, Moberg, & Van Eenennaam, ; Hurvitz, Jackson, Degani, & Levavi‐Sivan, ). As the normal distribution of sexes in sturgeons is 1:1 (female to male) (Mims & Shelton, ; Wuertz, Güralp, Pšenička, & Chebanov, ). Several studies addressing gynogenesis in sturgeon species suggest a chromosomal mechanism (Flynn, Matsuoka, Reith, Martin‐Robichaud, & Benfey, ; Shelton & Mims, ; Van Eenennaam, Van Eenennaam, Medrano, & Doroshov, ) that the female has been suggested as heterogametic (ZW) and males as homogametic sex (ZZ), due to the sex ratio of meiotic gynogens observed.…”

Section: Introductionmentioning

confidence: 99%

“…Several studies addressing gynogenesis in sturgeon species suggest a chromosomal mechanism (Flynn, Matsuoka, Reith, Martin‐Robichaud, & Benfey, ; Shelton & Mims, ; Van Eenennaam, Van Eenennaam, Medrano, & Doroshov, ) that the female has been suggested as heterogametic (ZW) and males as homogametic sex (ZZ), due to the sex ratio of meiotic gynogens observed. The percentage of males ranged between 18%–50% in white sturgeon ( Acipenser transmontanus https://en.wikipedia.org/wiki/John_Richardson_(naturalist), 1836) (Van Eenennaam et al, ), 20%–30% in hybrid bester (Omoto, Maebayashi, Adachi, Arai, & Yamauchi, ), 19% in Siberian sturgeon ( Acipenser baerii https://en.wikipedia.org/wiki/Johann_Friedrich_von_Brandt, 1869) (Fopp‐Bayat, ), 20% in paddlefish ( Polyodon spathula https://en.wikipedia.org/wiki/Bernard_Germain_de_Lac%C3%A9p%C3%A8de, 1797) (Shelton & Mims, ) and 28% in ship sturgeon ( A. nudiventris Lovetsky, 1828) (Saber et al, ), confirming a common sex determination mechanism (but not a sex determining master gene) among Acipenseriformes (Wuertz et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Masculinization of the gynogenetic juvenile ship sturgeon (Acipenser nudiventrisLovetsky, 1828) using 17α‐methyl testosterone

et al. 2019

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In sturgeons, the induction of gynogenesis and sex reversal could be important for potential production of neomale sturgeon and all‐female progeny for caviar production. The aim of this study was sex reversal of ship sturgeon (Acipenser nudiventris Lovetsky, 1828) gynogen into male sex. Five‐month‐old gynogens were sex reversed into male by including 17α‐methyl testosterone in their food for 7 months. Three treatments were considered as follows: (a) without treated (gynogen control), (b) 10 mg MT/kg diet, and (c) 50 mg MT/kg diet. All treatments (60 individuals) were sampled both the 30 and 36 months old and their sex was determined using classical histology method of gonad. The sex ratio of the progenies in the gynogen control were 73.3% female and 26.7% male. In treatment of 10 mg MT/kg feed, 18 specimens were studied that half of them (50%) showed pseudo‐testicular structure in the female gonad. That is 50% of the specimens were intersex, 27.7% were male and 22.3% were female. All of the fish fed by 50 mg MT/kg feed had been sex reversed to male. Sexual maturation of these fish had been recognized in stage III at 36 months old. In conclusion, 50 mg MT/kg feed found as effective dose for successful sex reversal in gynogenetic ship sturgeon.

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“…Over the years, wild sturgeon populations have diminished greatly due to overfishing and poaching combined with water pollution and a loss of natural habitats. This has resulted in the current status of the Acipenseridae family as one of the most endangered among all animal species [ 1 ]. The Russian sturgeon ( Acipenser gueldenstaedtii ), which originated in the Caspian Sea basin, is one of these critically endangered species and has relatively recently been introduced into Western European aquaculture [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Histological Evaluation of Gonad Impairments in Russian Sturgeon (Acipenser gueldenstaedtii) Reared in Recirculating Aquatic System (RAS)

Rzepkowska

Adamek‐Urbańska

Fajkowska

et al. 2020

Animals

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The aim of the study was to raise an issue concerning gonadal impairments in sturgeon reared in recirculating aquatic systems (RAS). In the present study, an in-depth histological evaluation in terms of gonadal pathologies was performed on over-4-year-old (1600 days post-hatching) Russian sturgeon (Acipenser gueldenstaedtii) reared under indoor RAS. A female-biased sex ratio, intersex occurrence, ovarian fat overgrowth, T-cell infiltration and follicle atresia were the most commonly observed disorders in the analyzed gonads. The combined processes of oocyte autophagy and follicular cell apoptosis were engaged in follicular atresia; however, atretic follicles showed a varied morphology, whereas oogonia and oocytes in the early stages of meiosis, as well as spermatogonia, underwent degeneration by apoptosis. The most severe pathology was observed in females with abundant intra-ovarian fat deposition. The extremely fatty ovaries were noted to lose the majority of ovarian follicles, which directly leads to fish sterility. The identified impairments might be related to estrogenic endocrine disruption, as feminization and unspecific vitellogenin synthesis were detected, although the sources of the observed pathologies can be diverse. Therefore, the presented research lays the groundwork for further studies on reproductive disorders in this prized and endangered fish species.

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Sex Determination in Sturgeon

Cited by 7 publications

References 112 publications

Primordial Germ Cell Migration and Histological and Molecular Characterization of Gonadal Differentiation in Pachón Cavefish <b><i>Astyanax mexicanus</i></b>

Primordial Germ Cell Migration and Histological and Molecular Characterization of Gonadal Differentiation in Pachón Cavefish <b><i>Astyanax mexicanus</i></b>

Masculinization of the gynogenetic juvenile ship sturgeon (Acipenser nudiventrisLovetsky, 1828) using 17α‐methyl testosterone

Histological Evaluation of Gonad Impairments in Russian Sturgeon (Acipenser gueldenstaedtii) Reared in Recirculating Aquatic System (RAS)

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