Sex Difference in Prolactin and Growth Hormone Cells in Mouse Adenohypophysis: Stereological, Morphometric, and Immunohistochemical Studies by Light and Electron Microscopy*

Sasaki, Fumihiko; Iwama, Yoshie

doi:10.1210/endo-123-2-905

Cited by 46 publications

(30 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…74% at 150 days. The number of GH cells purified from mature animals are in good agreement with previous studies (Sasaki & Iwama 1988, Gubkina et al 2001.…”

Section: Isolation Of Gh-secreting Cells By Facs Analysissupporting

confidence: 90%

Characterization of adherens junction protein expression and localization in pituitary cell networks

Chauvet

El-Yandouzi²,

Mathieu³

et al. 2009

Journal of Endocrinology

View full text Add to dashboard Cite

Our view of anterior pituitary organization has been altered with the recognition that folliculo-stellate (FS) and somatotroph cell populations form large-scale three-dimensional homotypic networks. This morphological cellular organization may optimize communication within the pituitary gland promoting coordinated pulsatile secretion adapted to physiological needs. The aim of this study was to identify the molecules involved in the formation and potential functional organization and/or signaling within these cell-cell networks. Here, we have focused on one class of cell adhesion molecules, the cadherins, since b-catenin has been detected in the GH cell network. We have characterized, by qPCR and immunohistochemistry, their cellular expression and distribution. We have also examined whether their expression could be modulated during pituitary tissue remodeling. The mouse anterior pituitary has a restricted and cell-type specific repertoire of cadherin expression: cadherin-11 is exclusively expressed in TSH cells; N-cadherin displays a ubiquitous expression pattern but with different levels of expression between endocrine cell types; E-cadherin is restricted to homotypic contacts between FS cells; while cadherin-18 is expressed both in somatotrophs and FS cells. Thus, each cell type presents a defined combinatorial expression of different subsets of cadherins. This cell-type specific cadherin expression profile emerges early during development and undergoes major changes during postnatal development. These results suggest the existence within the anterior pituitary of cell-cell contact signaling based on a defined pattern of cadherin expression, which may play a crucial role in cellular recognition during the formation and fate of pituitary cell homotypic networks.

show abstract

“…74% at 150 days. The number of GH cells purified from mature animals are in good agreement with previous studies (Sasaki & Iwama 1988, Gubkina et al 2001.…”

Section: Isolation Of Gh-secreting Cells By Facs Analysissupporting

confidence: 90%

Characterization of adherens junction protein expression and localization in pituitary cell networks

Chauvet

El-Yandouzi²,

Mathieu³

et al. 2009

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Such PRL-and GH-containing bihormonal cells have been identified in the human (12), bovine (13), goat (14), sheep (15), bat (16), rat (17), mouse (18), and musk shrew (19) anterior pituitary gland. To our knowledge, this is the first report of the existence of mammosomatotrophs within the avian pituitary gland.…”

Section: Discussionmentioning

confidence: 99%

Identification of Mammosomatotrophs in the Turkey Hen Pituitary: Increased Abundance during Hyperprolactinemia*

et al. 1998

View full text Add to dashboard Cite

We have previously reported that the hyperprolactinemia in incubating turkey hens is associated with recruitment of lactotrophs in the pituitary gland. In this study we have used double immunofluorescence and in situ hybridization histochemistry to 1) identify mammosomatotrophs in the anterior pituitary gland of egg-laying turkey hens and incubating hens, and 2) verify PRL gene expression within mammosomatotrophs by colocalizing PRL messenger RNA in GHimmunoreactive (ir) cells. The pituitaries of laying and incubating turkey hens were collected, and the midsagittal sections were dual labeled for either PRL and GH or PRL messenger RNA and GH. The plasma PRL concentrations were higher in incubating hens (231 Ϯ 10.6 ng/ml) than in laying hens (43 Ϯ 7.4 ng/ml; P Ͻ 0.01). In the midsagittal pituitary sections, mammosomatotrophs were predominantly found scattered in the caudal lobe of the anterior pituitary gland, in the ventral half of the cephalic lobe, and at the junction of cephalic and caudal lobes. In incubating hens, the proportion of mammosomatotrophs was 7.4 Ϯ 1.52% (mean Ϯ SEM) of the total number of GH-ir and/or PRL-ir cells counted, which was significantly higher (P Ͻ 0.05) than that found in laying hens (0.6 Ϯ 0.23%). Furthermore, PRL gene expression was observed in many GH-ir cells in the incubating hen pituitary gland. These data suggest that 1) mammosomatotrophs are present in the turkey pituitary gland, and 2) there is an increased abundance of mammosomatotrophs in the incubating turkey hen that may contribute to hyperprolactinemia. (Endocrinology 139: [781][782][783][784][785][786] 1998)

show abstract

“…This high proliferation of mammotrophs depends upon ovaries or ovarian estrogen, since ovariectomy decreased the high mitotic activity observed during estrus, while estrogen replacement increased mitotic activity. This sexual difference in mitotic activity is thought to lead to the sexual difference in mammotroph number (Takahashi and Kawashima, 1982;Sasaki and Iwama, 1988).…”

Section: Mammotroph Proliferationmentioning

confidence: 99%

Intrapituitary Regulatory System of Proliferation of Mammotrophs in the Pituitary Gland

Takahashi

2004

Zoological Science

View full text Add to dashboard Cite

-Anterior pituitary cells produce growth factors plus cytokines and their receptors. Although some of these pituitary growth factors and cytokines are known to be involved in the control of cell differentiation, proliferation and hormone production in the pituitary gland, their physiological roles remain unknown. Lots of evidence indicates that they are involved in the regulation of prolactin-secreting mammotroph cell proliferation. The regulation of mammotroph functions is a suitable system for understanding the intrapituitary regulatory system operated by growth factors and cytokines, since mammotrophs are the most actively proliferating cells in female pituitary glands. This review discusses the possible intrapituitary regulation of mammotroph differentiation and proliferation in rat and mouse pituitaries.

show abstract

Sex Difference in Prolactin and Growth Hormone Cells in Mouse Adenohypophysis: Stereological, Morphometric, and Immunohistochemical Studies by Light and Electron Microscopy*

Cited by 46 publications

References 24 publications

Characterization of adherens junction protein expression and localization in pituitary cell networks

Characterization of adherens junction protein expression and localization in pituitary cell networks

Identification of Mammosomatotrophs in the Turkey Hen Pituitary: Increased Abundance during Hyperprolactinemia*

Intrapituitary Regulatory System of Proliferation of Mammotrophs in the Pituitary Gland

Contact Info

Product

Resources

About