The purpose of this prospective, randomized, single-blinded, crossover study was to compare the pain of a traditional 1-stage inferior alveolar nerve (IAN) block injection to a 2-stage IAN block technique. Using a crossover design, 51 subjects randomly received, in a single-blinded manner, either the traditional IAN block or the 2-stage IAN block in 2 appointments spaced at least 1 week apart. For the 2-stage injection, the needle was inserted submucosally and 0.4 mL of 2% lidocaine with epinephrine was slowly given over 1 minute. After 5 minutes, the needle was reinserted and advanced to the target site (needle placement), and 1.8 mL of 2% lidocaine with epinephrine was deposited. For the traditional IAN block, following needle penetration, the needle was advanced while depositing 0.4 mL of 2% lidocaine with epinephrine (needle placement) and then 1.8 mL of 2% lidocaine with epinephrine was deposited at the target site. A Heft-Parker visual analogue scale was used to measure the pain of needle insertion, needle placement, and anesthetic solution deposition. There were no significant differences, as analyzed by Wilcoxon matchedpairs signed-ranks test, between needle insertion and solution deposition for the 2 techniques in men or women. However, there was significantly less pain with the 2-stage injection for needle placement in women. In conclusion, the 2-stage injection significantly reduced the pain of needle placement for women when compared to the traditional IAN technique.Key Words: Injection pain; Inferior alveolar nerve block; Lidocaine.T he injection of anesthetic solution for the inferior alveolar nerve (IAN) block has 3 phases: initial needle insertion through the alveolar mucosa, needle placement to the target site, and deposition of the anesthetic solution at the target site.The inferior alveolar nerve block has been associated with pain and discomfort. For the needle insertion phase, Nusstein and Beck, 1 in a retrospective study of 1635 IAN blocks, reported an incidence of moderate to severe pain ranging from 14 to 22%. For the deposition of the anesthetic solution at the target site, various authors 2-5 have reported that the incidence of moderate to severe pain ranged from 20 to 40%. Therefore, it would be advantageous to decrease the pain of the inferior alveolar nerve block.The 2-stage injection has been suggested by Walton and Torabinejad 6 and Levine 7 as a way to decrease the pain of injection. This method involves initial placement of anesthetic solution just under the mucosal surface. After a wait of several minutes for regional numbness, the injection is resumed and the remaining anesthetic solution is deposited at the target site. No clinical studies have addressed the 2-stage injection technique to reduce the pain of injection.The purpose of this prospective, randomized, singleblinded, crossover study was to compare the pain of a conventional inferior alveolar nerve block injection to that of a 2-stage inferior alveolar nerve block technique.