SEX DIFFERENCES IN FUNCTION AND DISTRIBUTION OF beta-ADRENOCEPTORS IN RABBIT URINARY BLADDER

Morita, Takashi; Masuda, Hitoshi; Tosaka, Akira; Ishizaka, Kazuhiro; Tsujii, Toshihiko; Kondo, Shun

doi:10.1016/s0022-5347(01)63982-x

Cited by 16 publications

(10 citation statements)

References 9 publications

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“…In a previous study (Atalık et al, 1999), we observed intracellular Ca 2+ pools may play a functional role in the cooling-induced contractions of rabbit detrusor smooth muscle. Furthermore, in literature sex-related differences in function and distribution of beta-adrenoceptors in rabbit urinary bladder (Morita et al, 1998). Although female rats were used in our experiments, the findings were in line with other reports in male rats (Hellstrom et al, 1991;Mustafa and Thulesius, 1999), indicating that there are not sex-related differences in the function of rat detrusor during cooling.…”

Section: Discussionsupporting

confidence: 91%

Diabetes mellitus- and cooling-induced bladder contraction: an in vitro study

Atalık

Okudan

Gökbel

et al. 2010

J. Smooth Muscle Res.

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The effects of diabetes mellitus during cooling on ACh-and KCl-induced responses were investigated in rat urinary bladder. Diabetes was induced in the rats by 50 mg/kg streptozotocin via an intraperitoneal injection. Rats' body and bladder weights were measured. The isometric tension to ACh (10 -9 -3 × 10 -4 M) and KCl (5-100 mM) in strips of urinary detrusor muscle of diabetic and non-diabetic rats, in organ baths at 37 and 28ºC were recorded. The body weights were significantly decreased and the bladder weights increased in STZ-induced diabetic group compared to the non-diabetic group. ACh and KCl caused concentration-dependent contractions of urinary bladders from non-diabetic and STZ-induced diabetic rats. During cooling, the sensitivity and the maximal response were significantly higher than those during 37ºC, both in non-diabetic and diabetic preparations. Cooling of detrusor muscle preparations induces a graded contraction inversely proportional to the temperature in diabetic rats. It may be assumed that the cooling response involves the same mechanisms in the diabetic and non-diabetic animals.

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Section: Discussionsupporting

confidence: 91%

Diabetes mellitus- and cooling-induced bladder contraction: an in vitro study

Atalık

Okudan

Gökbel

et al. 2010

J. Smooth Muscle Res.

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“…Interestingly, the efficacy of isoprenaline had also been numerically smaller in female as compared to male rats in a previous study; while this trend had consistently been observed with four different agonists including isoprenaline, it had reached statistical significance only for the partial agonists BRL 37,344 and CGP 12,177 (Frazier et al, 2006). On the other hand, a study in rabbits did not detect gender differences in isoprenaline-induced detrusor relaxation, but within that study relaxation responses in the trigone were significantly greater in female than male samples (Morita et al, 1998). Thus, there is insufficient evidence for solid conclusions regarding a possible difference in β-adrenoceptor-mediated detrusor relaxation.…”

Section: Discussionmentioning

confidence: 95%

Human Urinary Bladder Strip Relaxation by the β-Adrenoceptor Agonist Isoprenaline: Methodological Considerations and Effects of Gender and Age

Schneider¹,

Fetscher²,

Michel³

2011

Front. Pharmacol.

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The present study was primarily designed to explore various methodological aspects related to organ bath experiments evaluating human detrusor relaxation by the β-adrenoceptor agonist isoprenaline. Data are based upon a series of 30 consecutive patients, and this cohort was also used to explore possible effects of gender and age. KCl-induced contraction was related to strip length but not weight or cross-sectional area, indicating that the former is most suitable for data normalization. Storage of detrusor strips in cold buffer for up to 2 days did not affect contractile responses to KCl or efficacy of isoprenaline to cause relaxation but significantly affected the isoprenaline potency. No such alterations were observed with up to 1 day of cold storage. The type (KCl vs. passive tension) or strength of contractile stimulus had only minor effects on isoprenaline responses although these differences reached statistical significance in some cases. Similarly, gender and age had only minor if any effects on KCl-induced contraction or isoprenaline-induced relaxation, but the current data are too limited for robust conclusions. In summary we have evaluated experimental conditions for the testing of human detrusor strip contraction and relaxation which should be useful for future larger studies.

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“…In view of these observations the importance of water intake and consequently urine production, on the function of the bladder has been extended by Ohnishi et al [1999] who demonstrated explicitly that diuresis causes an increase in contractile strength consequently protecting the bladder from the contractile dysfunction associated with partial obstruction. In addition to the in¯uence of diuresis noted above, it is appropriate to consider that there are gender differences in the central and peripheral distribution of receptors [Latifpour et al, 1990;Vaughan and Satchel, 1995;Morita et al, 1998] that may affect the contractile function of the bladder. In addition to variations in a and b receptors contractility may be in¯uenced by estrogen and NO shown to reduce bladder blood¯ow [Pontari and Ruggieri, 1999] and whole body production of NO is greater in healthy women than in men [Forte et al, 1998].…”

Section: Discussionmentioning

confidence: 99%

Influence of gender on the diurnal variation of urine production and micturition characteristics of the rat

Schmidt¹,

Yoshimura

et al. 2001

Neurourology and Urodynamics

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The diurnal variation in the frequency/volume characteristics of male and female conscious rats was evaluated with reference to fluid consumption and urine production. Baseline values of the micturition volume and frequency of nine male and 10 female SD adult rats were measured over a 24-hour time period. The level of initial hydration conditions was standardized with 5 ml of water administered orally. With animals in a metabolism chamber having free access to water, the total volume of water consumed, the frequency/volume characteristics during micturition and the urine production rate were derived from the measurements of voided volume as detected by a digital balance. To establish reliability of measurements two separate micturition studies were done per rat at an interval of 1 week. Mean frequency of micturition and mean volume voided per micturition and urine production rate were computed in 3-hour time bins and represented over the 24-hour period. In addition the mean values of the number of micturitions and mean micturated volumes during the day/dark cycle were evaluated. The results show significant gender specificity in water consumption, urine production, and diurnal variations in micturition frequency/volume characteristics. Females consistently consume significantly larger amounts of water (83%) than males while urine production rate was correspondingly higher in females. It is concluded that water consumption and urine production are gender-specific. Because higher volumes of water are imbibed by females than males, the frequency/volume characteristic of micturition in the rat is also gender-specific. Data suggest that the volume voided per micturition depends on the urine production rate.

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SEX DIFFERENCES IN FUNCTION AND DISTRIBUTION OF beta-ADRENOCEPTORS IN RABBIT URINARY BLADDER

Abstract: These findings demonstrate that there are sex-related differences in the function and the distribution of beta-adrenoceptors in rabbit trigonal smooth muscles.

Cited by 16 publications

References 9 publications

Diabetes mellitus- and cooling-induced bladder contraction: an in vitro study

Diabetes mellitus- and cooling-induced bladder contraction: an in vitro study

Human Urinary Bladder Strip Relaxation by the β-Adrenoceptor Agonist Isoprenaline: Methodological Considerations and Effects of Gender and Age

Influence of gender on the diurnal variation of urine production and micturition characteristics of the rat

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