Sex differences in hippocampal structural plasticity and glycosaminoglycan disaccharide levels after neonatal handling

Hashimoto, Joel G.; Singer, Mo L; Goeke, Calla M; Zhang, Fuming; Song, Yu; Xia, Ke; Linhardt, Robert J.; Guizzetti, Marina

doi:10.1016/j.expneurol.2022.114313

Experimental Neurology

2023

DOI: 10.1016/j.expneurol.2022.114313

|View full text |Cite

Sex differences in hippocampal structural plasticity and glycosaminoglycan disaccharide levels after neonatal handling

Joel G. Hashimoto

Mo L Singer

Calla M Goeke

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Preprint2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Astrocyte extracellular matrix modulates neuronal dendritic development

Hashimoto,

Margolies,

Zhang

et al. 2024

Preprint

View full text Add to dashboard Cite

Major developmental events occurring in the hippocampus during the third trimester of human gestation and neonatally in altricial rodents include rapid and synchronized dendritic arborization and astrocyte proliferation and maturation. We tested the hypothesis that signals sent by developing astrocytes to developing neurons modulate dendritic developmentin vivo. We altered neuronal development by neonatal (third trimester-equivalent) ethanol exposure in mice; this treatment increased dendritic arborization in hippocampal pyramidal neurons. We next assessed concurrent changes in the mouse astrocyte translatome by translating ribosomal affinity purification (TRAP)-seq. We followed up on ethanol-inhibition of astrocyteChpf2andChsy1gene translation because these genes encode for biosynthetic enzymes of chondroitin sulfate glycosaminoglycan (CS-GAG) chains (extracellular matrix components that inhibit neuronal development and plasticity) and have not been explored before for their roles in dendritic arborization. We report thatChpf2andChsy1are enriched in astrocytes and their translation is inhibited by ethanol, which also reduces the levels of CS-GAGs measured by Liquid Chromatography/Mass Spectrometry. Finally, astrocyte-conditioned medium derived fromChfp2-silenced astrocytes increased neurite branching of hippocampal neuronsin vitro. These results demonstrate that CS-GAG biosynthetic enzymes in astrocytes regulates dendritic arborization in developing neurons.

show abstract

Astrocyte extracellular matrix modulates neuronal dendritic development

Hashimoto,

Margolies,

Zhang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Sex differences in brain cell-type specific chromatin accessibility in schizophrenia

Roussos,

Ma,

Girdhar

et al. 2024

Preprint

View full text Add to dashboard Cite

Our understanding of the sex-specific role of the non-coding genome in serious mental illness remains largely incomplete. To address this gap, we explored sex differences in 1,393 chromatin accessibility profiles, derived from neuronal and non-neuronal nuclei of two distinct cortical regions from 234 cases with serious mental illness and 235 controls. We identified sex-specific enhancer-promoter interactions and showed that they regulate genes involved in X-chromosome inactivation (XCI). Examining chromosomal conformation allowed us to identify sex-specific cis- and trans-regulatory domains (CRDs and TRDs). Co-localization of sex-specific TRDs with schizophrenia common risk variants pinpointed male-specific regulatory regions controlling a number of metabolic pathways. Additionally, enhancers from female-specific TRDs were found to regulate two genes known to escape XCI, (XIST and JPX), underlying the importance of TRDs in deciphering sex differences in schizophrenia. Overall, these findings provide extensive characterization of sex differences in the brain epigenome and disease-associated regulomes.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Sex differences in hippocampal structural plasticity and glycosaminoglycan disaccharide levels after neonatal handling

Cited by 2 publications

References 60 publications

Astrocyte extracellular matrix modulates neuronal dendritic development

Astrocyte extracellular matrix modulates neuronal dendritic development

Sex differences in brain cell-type specific chromatin accessibility in schizophrenia

Contact Info

Product

Resources

About