Sex differences in muscle health in simulated micro- and partial-gravity environments in rats

Rosa-Caldwell, Megan E.; Mortreux, Marie; Wadhwa, Anna; Kaiser, Ursula B.; Sung, Dong-Min; Bouxsein, Mary L.; Rutkove, Seward B.

doi:10.1016/j.smhs.2023.09.002

Cited by 1 publication

(1 citation statement)

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“…Estrous cycle was analyzed with custom statistical code to evaluate the differences between groups at each time point with a Holm's post‐hoc used to control for Type 1 error rate. We have previously used versions of this code in previous manuscripts to increase statistical power for specific comparisons of interest (Rosa‐Caldwell, Mortreux, et al., 2023a , 2023b ). Other data were analyzed with t ‐test.…”

Section: Methodsmentioning

confidence: 99%

A refined rodent model of anorexia nervosa: Simulating state‐specific effects of caloric restriction and weight restoration

Rosa‐Caldwell,

Breithaupt,

Kaiser

et al. 2024

Physiological Reports

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Current rodent models of anorexia nervosa (AN) often have accelerated weight loss that often do not allow for investigation of physiological ramifications of prolonged low weight status characteristic of AN. The purpose of this project was to refine a rodent model of AN to extend the duration of low weight status and allow for investigation of recovery. Eight‐week‐old female Sprague Dawley rats underwent 50%–60% food restriction for 30 days. Rats were group‐housed except during feeding, where AN rats were individually housed and given up to 2 h to consume food. Control (CON) rats were allowed to consume food ad libitum. To simulate recovery, a separate cohort of animals underwent the same food restriction protocol for 30 days, then rats (AN‐R) were allowed to consume food ad libitum to facilitate weight recovery for an additional 30 days. AN‐R rats were compared to age matched controls (CON‐R). AN rats lost ~15% bodyweight and were ~30% lighter than CON. Compared to CON, AN rats had ~35% lower fat content, ~18% lower bone mineral density, ~22% smaller plantaris muscle mass and ~52% smaller ovaries. Upon reintroduction of food, AN‐R rats achieved comparable bodyweights to CON‐R rats after ~10 days. However, after 30 days of recovery, AN‐R rats still had ~14% lower bone mineral density and ~11% smaller plantaris mass and ~21% smaller ovaries. This refinement of rodent AN results in physiological side effects of AN without reaching excessive weight loss requiring euthanasia. Moreover, some physiological consequences of simulated AN are not resolved with weight restoration.

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Section: Methodsmentioning

confidence: 99%