Sex differences in skeletal muscle size, function, and myosin heavy chain isoform expression during post‐injury regeneration in mice

You, Jae‐Sung; Barai, Pallob; Chen, Jie

doi:10.14814/phy2.15791

Cited by 7 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exercise was shown to be superior to caloric restriction in terms of improving skeletal muscle fatty acid oxidation and locomotor activity, whereas caloric restriction is superior to exercise in terms of decreasing oxidative stress and increasing antioxidative capacity in skeletal muscle. However, these findings may not be applicable to females since the skeletal muscle structure and skeletal muscle metabolism of males and females are different [66][67][68], and sex hormones play crucial roles in skeletal muscle metabolic and mechanical functions [69][70][71]. Hence, future studies investigating the beneficial effects of exercise and caloric restriction on the amelioration of skeletal muscle aging in females are needed.…”

Section: Discussionmentioning

confidence: 99%

Exercise and Caloric Restriction Exert Different Benefits on Skeletal Muscle Metabolism in Aging Condition

Thonusin,

Pantiya,

Kongkaew

et al. 2023

Nutrients

View full text Add to dashboard Cite

Exercise and caloric restriction improve skeletal muscle metabolism. However, the benefits of exercise and caloric restriction on skeletal muscle metabolism in aging have never been compared. Seven-week-old male Wistar rats (n = 24) were divided into 4 groups (n = 6 per group) to receive either normal saline solution for 28 weeks, 150 mg/kg/day of D-galactose for 28 weeks to induce premature aging, 150 mg/kg/day of D-galactose for 28 weeks plus exercise for 16 weeks (week 13–28), or 150 mg/kg/day of D-galactose for 28 weeks plus 30% caloric restriction for 16 weeks (week 13–28). The 17-month-old rats (n = 6) were also injected with normal saline solution for 28 weeks as the naturally aged controls. At the end of week 28, total walking distance and fatty acid and carbohydrate oxidation during physical activity were determined. Then, all rats were euthanized for the collection of blood and tibialis anterior muscle. The results showed that D-galactose successfully mimicked the natural aging of skeletal muscle. Exercise and caloric restriction equally improved carbohydrate oxidation during physical activity and myogenesis. However, exercise was superior to caloric restriction in terms of improving fatty acid oxidation and oxidative phosphorylation. Interestingly, caloric restriction decreased oxidative stress, whereas exercise increased oxidative stress of skeletal muscle. All of these findings indicated that the benefits of exercise and caloric restriction on skeletal muscle metabolism during aging were different, and therefore the combination of exercise and caloric restriction might provide greater efficacy in ameliorating skeletal muscle aging.

show abstract

Section: Discussionmentioning

confidence: 99%

Exercise and Caloric Restriction Exert Different Benefits on Skeletal Muscle Metabolism in Aging Condition

Thonusin,

Pantiya,

Kongkaew

et al. 2023

Nutrients

View full text Add to dashboard Cite

show abstract

“…Ten‐week‐old C57BL/6 mice (Japan SLC, Shizuoka, Japan) were used for this study. Furthermore, it is expected that results will vary by sex, including hormonal effects (Serra et al., 2013 ; You et al., 2023 ). Therefore, our experiments were specifically conducted using male mice.…”

Section: Methodsmentioning

confidence: 99%

Histological and biochemical changes in lymphatic vessels after skeletal muscle injury induced by lengthening contraction in male mice

Tamura,

Kawashima,

et al. 2024

Physiological Reports

View full text Add to dashboard Cite

Lymphatic vessels are actively involved in the recovery process of inflamed tissues. However, the changes in intramuscular lymphatic vessels during inflammation caused by skeletal muscle injury remain unclear. Therefore, the purpose of this study was to clarify the changes in lymphatic vessels after skeletal muscle injury. The left tibialis anterior muscles of male mice were subjected to lengthening contractions (LC) for inducing skeletal muscle injury, and samples were collected on Days 2, 4, and 7 for examining changes in both the skeletal muscles and intramuscular lymphatic vessels. With hematoxylin–eosin staining, the inflammatory response was observed in myofibers on Days 2 and 4 after LC, whereas regeneration of myofibers was found on Day 7 after LC. The number and area of intramuscular lymphatic vessels analyzed by immunohistochemical staining with an antibody against lymphatic vessel endothelial hyaluronan receptor 1 were significantly increased only on Day 4 after LC. Based on the abovementioned results, intramuscular lymphatic vessels undergo morphological changes such as increase under the state of muscle inflammation. This study demonstrated that the morphology of intramuscular lymphatic vessels undergoes significant changes during the initial recovery phase following skeletal muscle injury.

show abstract

“…Cell count data were normalized by the number of cells on the day of the experimental peak to allow for comparison between experiments and simulations. For CSA, the experimental and model outcomes were normalized using fold-change from pre-injury to compare model-simulated with experimental CSA, as percent change from baseline is commonly used experimentally ( Pratt et al, 2015 ; You et al, 2023 ). Model cell counts were normalized by the number of cells at the peak timepoint in the experimental data.…”

Section: Methodsmentioning

confidence: 99%

Agent-based model demonstrates the impact of nonlinear, complex interactions between cytokines on muscle regeneration

Haase,

Comlekoglu,

Petrucciani

et al. 2024

eLife

View full text Add to dashboard Cite

Muscle regeneration is a complex process due to dynamic and multiscale biochemical and cellular interactions, making it difficult to determine optimal treatments for muscle injury using experimental approaches alone. To understand the degree to which individual cellular behaviors impact endogenous mechanisms of muscle recovery, we developed an agent-based model (ABM) using the Cellular Potts framework to simulate the dynamic microenvironment of a cross-section of murine skeletal muscle tissue. We referenced more than 100 published studies to define over 100 parameters and rules that dictate the behavior of muscle fibers, satellite stem cells (SSC), fibroblasts, neutrophils, macrophages, microvessels, and lymphatic vessels, as well as their interactions with each other and the microenvironment. We utilized parameter density estimation to calibrate the model to temporal biological datasets describing cross-sectional area (CSA) recovery, SSC, and fibroblast cell counts at multiple time points following injury. The calibrated model was validated by comparison of other model outputs (macrophage, neutrophil, and capillaries counts) to experimental observations. Predictions for eight model perturbations that varied cell or cytokine input conditions were compared to published experimental studies to validate model predictive capabilities. We used Latin hypercube sampling and partial rank correlation coefficient to identify in silico perturbations of cytokine diffusion coefficients and decay rates to enhance CSA recovery. This analysis suggests that combined alterations of specific cytokine decay and diffusion parameters result in greater fibroblast and SSC proliferation compared to individual perturbations with a 13% increase in CSA recovery compared to unaltered regeneration at 28 days. These results enable guided development of therapeutic strategies that similarly alter muscle physiology (i.e. converting ECM-bound cytokines into freely diffusible forms as studied in cancer therapeutics or delivery of exogenous cytokines) during regeneration to enhance muscle recovery after injury.

show abstract

Sex differences in skeletal muscle size, function, and myosin heavy chain isoform expression during post‐injury regeneration in mice

Cited by 7 publications

References 16 publications

Exercise and Caloric Restriction Exert Different Benefits on Skeletal Muscle Metabolism in Aging Condition

Exercise and Caloric Restriction Exert Different Benefits on Skeletal Muscle Metabolism in Aging Condition

Histological and biochemical changes in lymphatic vessels after skeletal muscle injury induced by lengthening contraction in male mice

Agent-based model demonstrates the impact of nonlinear, complex interactions between cytokines on muscle regeneration

Contact Info

Product

Resources

About