Sex differences in socioemotional behavior and changes in ventral hippocampal transcription across aging in C57Bl/6J mice

Baumgartner, Nina E.; Biraud, Mandy; Lucas, Elizabeth K.

doi:10.1016/j.neurobiolaging.2023.05.015

Cited by 10 publications

(6 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Age-related changes in affective behaviors in early vs. late adulthood have been reported in mice (Flurkey et al, 2007;Shoji and Miyakawa, 2019;Baumgartner et al, 2023). Young (2-3 month old; mo) C57BL/6 J male mice display greater anxiety-like behaviors compared to older adult, late middle age and aged mice (Ammassari-Teule et al, 1994;Shoji and Miyakawa, 2019).…”

Section: Introductionmentioning

confidence: 92%

“…Young (2-3 month old; mo) C57BL/6 J male mice display greater anxiety-like behaviors compared to older adult, late middle age and aged mice (Ammassari-Teule et al, 1994;Shoji and Miyakawa, 2019). Although this remains unclear, since other studies provide evidence of greater anxiety-like behaviors in aged compared to young adult mice (Shoji and Miyakawa, 2019;Baumgartner et al, 2023). Nevertheless, the expression of fearrelated associative memory (von Bohlen und Halbach et al, 2006;Evans et al, 2021;Ferreira et al, 2023), reward sensitivity (Ammassari-Teule et al, 1994;Malatynska et al, 2012), sociability and social recognition (Shoji and Miyakawa, 2019), and learned helplessness on a Porsolt test (Ferreira et al, 2023) are all observed to vary in young vs. older adulthood mice.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Age-related differences in affective behaviors in mice: possible role of prefrontal cortical-hippocampal functional connectivity and metabolomic profiles

Febo,

Mahar,

Rodriguez

et al. 2024

Front. Aging Neurosci.

View full text Add to dashboard Cite

IntroductionThe differential expression of emotional reactivity from early to late adulthood may involve maturation of prefrontal cortical responses to negative valence stimuli. In mice, age-related changes in affective behaviors have been reported, but the functional neural circuitry warrants further investigation.MethodsWe assessed age variations in affective behaviors and functional connectivity in male and female C57BL6/J mice. Mice aged 10, 30 and 60 weeks (wo) were tested over 8 weeks for open field activity, sucrose preference, social interactions, fear conditioning, and functional neuroimaging. Prefrontal cortical and hippocampal tissues were excised for metabolomics.ResultsOur results indicate that young and old mice differ significantly in affective behavioral, functional connectome and prefrontal cortical-hippocampal metabolome. Young mice show a greater responsivity to novel environmental and social stimuli compared to older mice. Conversely, late middle-aged mice (60wo group) display variable patterns of fear conditioning and during re-testing in a modified context. Functional connectivity between a temporal cortical/auditory cortex network and subregions of the anterior cingulate cortex and ventral hippocampus, and a greater network modularity and assortative mixing of nodes was stronger in young versus older adult mice. Metabolome analyses identified differences in several essential amino acids between 10wo mice and the other age groups.DiscussionThe results support differential expression of ‘emotionality’ across distinct stages of the mouse lifespan involving greater prefrontal-hippocampal connectivity and neurochemistry.

show abstract

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Age-related differences in affective behaviors in mice: possible role of prefrontal cortical-hippocampal functional connectivity and metabolomic profiles

Febo,

Mahar,

Rodriguez

et al. 2024

Front. Aging Neurosci.

View full text Add to dashboard Cite

show abstract

“…Maturational changes in affective behaviors in early versus late adulthood have been reported in rodents (Baumgartner et al, 2023; Flurkey et al, 2007; Shoji and Miyakawa, 2019). Young (2–3-month-old; mo) C57BL/6J male mice display greater anxiety-like behaviors compared to older adult, late middle age and aged mice (Ammassari-Teule et al, 1994; Shoji and Miyakawa, 2019).…”

Section: Introductionmentioning

confidence: 96%

“…Young (2-3-month-old; mo) C57BL/6J male mice display greater anxiety-like behaviors compared to older adult, late middle age and aged mice (Ammassari-Teule et al, 1994;Shoji and Miyakawa, 2019). Although this remains unclear, since other studies provide evidence of greater anxiety-like behaviors in aged compared to young adult mice (Shoji and Miyakawa, 2019;Baumgartner et al, 2023). Nevertheless, the expression of fear-related associative memory (von Bohlen und Halbach et al, 2006;Evans et al, 2021;Ferreira et al, 2023), reward sensitivity (Ammassari-Teule et al, 1994;Malatynska et al, 2012), sociability and social recognition (Shoji and Miyakawa, 2019), and learned helplessness on a Porsolt test (Ferreira et al, 2023) are all observed to vary in young versus older adulthood mice.…”

Section: Introductionmentioning

confidence: 99%

Age-Related Differences in Affective Behaviors in Mice: Possible Role of Prefrontal Cortical-Hippocampal Functional Connectivity and Metabolomic Profiles

Febo,

Mahar,

Rodriguez

et al. 2023

Preprint

View full text Add to dashboard Cite

Differences in affective behavioral expression from early to late adulthood is thought to involve changes in frontal cortical responsiveness to negative valence stimuli. In mice, similar maturational changes in affective behaviors have also been reported but the functional neural circuitry remains unclear. In the present study we investigated age variations in affective behaviors and functional connectivity in male and female C57BL6/J mice. Mice aged 10, 30 and 60 weeks (wo) were tested over 8 weeks for open field activity, sucrose reward preference, social interactions and fear conditioning and functional neuroimaging. Frontal cortical and hippocampal tissues were excised for metabolomics analysis. Our results indicate that young 10wo mice display greater levels of anxiety-like locomotor behavior and develop robust fear conditioning compared to older adult and late middle-aged mice (30-60wo). This was accompanied by greater functional connectivity between a temporal cortical/auditory cortex network and subregions of the anterior cingulate cortex and ventral hippocampus, and a greater network modularity and assortative mixing of nodes in young versus older adult mice. Metabolome analyses identified differences in several essential amino acids between 10wo mice and the other age groups. The results support high ‘emotionality’ in younger versus older adult mice involving greater prefrontal-hippocampal connectivity.

show abstract

“…В опытах на стареющих мышах C57Bl/6 показан антиамнестический эффект аффинно-очищенных поликлональных антител к глутамату при их интраназальном введении [21]. Данные литературы свидетельствуют об усилении тревожного поведения у стареющих мышей [25].…”

Section: Introductionunclassified

Effects of glutamate antibodies and F(ab´)2 fragments of glutamate antibodies on the anxiety level in aging C57Bl/6 mice

Ветрилэ,

Захарова,

Лобанов

et al. 2023

Zhurnal «Patologicheskaia Fiziologiia I Eksperimental`naia Terapiia»

View full text Add to dashboard Cite

Введение. Высокая степень личностных тревожных расстройств выявляется у 96% пожилых и у 100% людей старческого возраста, а у 50% пожилых и 56% лиц старше 75 лет наблюдается повышение реактивной тревожности. Несмотря на наличие большого арсенала фармакологических средств для лечения тревожных расстройств, перспективным остается разработка лекарственных препаратов на основе антител и их фрагментов, благодаря таким свойствам, как специфичность, и метаболическая активность. Ранее в опытах на мышах BALB/C было показано снижение уровня тревожности при однократном внутрибрюшинном введении антител к глутамату. В опытах на стареющих мышах и на экспериментальных моделях болезни Альцгеймера, показан антиамнестический эффект при интраназальном введении аффинно-очищенных поликлональных антител к глутамату. Цель исследования – изучение влияния интраназального введения антител к глутамату и F(ab´)2 фрагментов антител к глутамату на уровень тревожности у стареющих мышей C57Bl/6. Методика. Исследование выполнено на мышах линии C57Bl/6 в возрасте 12 мес. Мыши были разделены на три группы: две опытные группы получали интраназально растворенные в физиологическом растворе поликлональные моноспецифические антитела к глутамату (АТ- ГЛУ) и F(ab´)2 фрагменты АТ- ГЛУ соответственно в дозе 250 мкг/кг в объеме 4 мкл в ежедневно в течение 3 дней. Мыши контрольной группы получали интраназально физиологический раствор в том же объеме. Оценивали поведенческую активность мышей в тесте «Открытое поле» и уровень тревожности в условиях теста «приподнятый крестообразный лабиринт». Результаты. Интраназальное введение стареющим мышам АТ-ГЛУ и F(ab´)2 фрагментов АТ-ГЛУ приводило к значимому увеличению количества посещений и времени пребывания в центре поля, к снижению времени пребывания в углах и увеличению количества стоек в углах открытого поля при сравнении с животными группы контроля. Анализ результатов тестирования в приподнятом крестообразном лабиринте показал, что мыши, получавшие интраназально АТ-ГЛУ и F(ab´)2 фрагменты АТ-ГЛУ, существенно увеличивали время, проведенное в открытых рукавах лабиринта, а также число реакций «свешивания» (заглядывание под лабиринт) по сравнению с мышами контрольной группы, что свидетельствует о снижении уровня тревожности и страха. Заключение. Полученные данные свидетельствуют о снижении уровня тревожности и страха у стареющих мышей C57Bl/6, получавших антитела к глутамату и F(ab´)2 фрагменты антител к глутамату. Introduction. Anxiety disorder occurs in approximately 15-25% of the adult population. A high degree of anxious personality disorders was found in 96% of old people and in 100% of very old people, whereas 50% of old people and 56% of people older than 75 had increased reactive anxiety. In persistent anxiety, production of β-amyloid increases and contributes to the development of neurodegenerative diseases of old age. Despite the availability of a large arsenal of pharmacological agents for the treatment of anxiety disorders, development of drugs based on antibodies and their fragments is promising due to their specificity, activity, and metabolic activity. Previous experiments on BALB/C mice showed a decrease in the anxiety level after a single intraperitoneal injection of glutamate antibodies. Experiments on aging mice and experimental models of Alzheimer’s disease demonstrated an anti-amnesic effect of affinity-purified polyclonal glutamate antibodies administered intranasally. The aim of the study was to evaluate the effect of intranasal glutamate antibodies and F(ab´)2 fragments of glutamate antibodies on the level of anxiety in aging C57Bl/6 mice. Methods. The study was performed on 12-month-old C57Bl/6 mice. Mice were divided into three groups: two experimental groups received polyclonal monospecific antibodies to glutamate (AT- GLU) and F(ab´)2 fragments of AT- GLU. The agents were dissolved in saline and administered intranasally at a dose of 250 µg/kg in a volume of 4 µl, daily for 3 days. Mice of the control group received intranasal saline in the same volume. The behavioral activity of mice was assessed by the open field test and the level of anxiety by the elevated plus maze test. Results. Intranasal administration of AT- GLU and F(ab´)2 fragments of AT-GLU to aging mice resulted in a significant increase in the number of visits to and the time spent in the center of the open field, a decrease in the time spent in the corners, and an increase in the number of rears in the corners of the open field compared to the control group. The elevated plus maze test showed that mice treated with AT- GLU and F(ab´)2 fragments of AT- GLU significantly increased the time spent in the open arms of the maze, as well as the number of unprotected head dips compared to the control group, which indicated a decrease in anxiety and fear. Conclusion. The study results indicated a possible decrease in the level of anxiety and fear in aging C57Bl/6 mice treated with glutamate antibodies and F(ab´)2 fragments of glutamate antibodies, which suggested an anxiolytic effect of glutamate antibodies and their F(ab´)2 fragments.

show abstract

Sex differences in socioemotional behavior and changes in ventral hippocampal transcription across aging in C57Bl/6J mice

Cited by 10 publications

References 103 publications

Age-related differences in affective behaviors in mice: possible role of prefrontal cortical-hippocampal functional connectivity and metabolomic profiles

Age-related differences in affective behaviors in mice: possible role of prefrontal cortical-hippocampal functional connectivity and metabolomic profiles

Age-Related Differences in Affective Behaviors in Mice: Possible Role of Prefrontal Cortical-Hippocampal Functional Connectivity and Metabolomic Profiles

Effects of glutamate antibodies and F(ab´)2 fragments of glutamate antibodies on the anxiety level in aging C57Bl/6 mice

Contact Info

Product

Resources

About