Sex differences in the development of hepatic steatosis in cafeteria diet-induced obesity in young mice

Gasparin, Fabiana Rodrigues Silva; Carreño, Fernando; Mewes, Juliana Morais; Gilglioni, Eduardo Hideo; Pagadigorria, Clairce Luzia Salgueiro; Natali, Maria Raquel Marçal; Utsunomiya, Karina Sayuri; Constantin, Rodrigo Polimeni; Ouchida, Amanda Tomie; Curti, Carlos; Gaemers, Ingrid C.; Elferink, Ronald Petrus Johannes Oude; Constantin, Jorgete; Ishii–Iwamoto, Emy Luiza

doi:10.1016/j.bbadis.2018.04.004

Cited by 46 publications

(68 citation statements)

References 98 publications

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“…Previously, a sex-specific activation of Fgf21 expression was observed in humans and animals with various metabolic diseases (Wang et al, 2017;Chukijrungroat et al, 2017;Lee et al, 2016;Gasparin et al, 2018). Our results are in line with data from Gasparin and coauthors (Gasparin et al, 2018), who found that DIO increased Fgf21 mRNA expression in the liver, WAT and BAT in male, but not female mice. The new finding of our study is demonstration of a female-specific activation of FGF21 signaling in response to the natural physiological states fasting and refeeding.…”

Section: Discussionsupporting

confidence: 92%

Sex Dimorphism in the Fgf21 Gene Expression in Liver and Adipose Tissues is Dependent on the Metabolic Condition

Бажан¹,

Jakovleva²,

Balyibina³

et al. 2019

OnLine Journal of Biological Sciences

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Fibroblast growth factor-21 (FGF21) beneficially affects carbohydrate and lipid metabolism. Previously, a sex-specific activation of Fgf21 expression was observed in humans and animals with metabolic diseases. It is unknown whether the sex differences in the Fgf21 expression are manifested in response to the natural physiological situations of fasting and refeeding. The aim of this work was to determine liver, White Adipose Tissue (WAT) and Brown Adipose Tissue (BAT) expression of genes related to FGF21 signaling in response to 24 h fasting, 6 h refeeding (after 24 h fasting) and Diet-Induced Obesity (DIO) in C57Bl mice of both sexes. Obesity was induced by the consumption of palatable food for 10 weeks. mRNA levels of peroxisome proliferator-activated receptor-a and-γ (Pparα, Pparγ), FGF21 (Fgf21), coactivator of FGF receptors (Klb) and transcriptional coactivator (Pgc-1α) were measured by RT-PCR. The study showed that the fasting-induced increases in hepatic Fgf21 gene expression and circulating FGF21 levels, as well as refeeding-induced increases in local WAT and BAT Fgf21 gene expression, were biased toward females. DIO-induced increase in circulating FGF21 levels, as well as in Fgf21 gene expression in the liver and BAT, were biased toward males. Considering that FGF21 is a novel metabolic regulator of energy homeostasis, sex differences in the responses to anabolic and catabolic stimulus could have translational implications for novel therapeutic outcomes.

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Section: Discussionsupporting

confidence: 92%

Sex Dimorphism in the Fgf21 Gene Expression in Liver and Adipose Tissues is Dependent on the Metabolic Condition

Бажан¹,

Jakovleva²,

Balyibina³

et al. 2019

OnLine Journal of Biological Sciences

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“…This histological classical staining was performed in the LAL muscle of all the experimental groups and the controls at the end of the period of exposure (6 weeks). Sudan III stains lipids orange-red [ 27 ]. The LAL muscles were extracted and fixed in formalin.…”

Section: Methodsmentioning

confidence: 99%

Effects of a Fat-Rich Diet on the Spontaneous Release of Acetylcholine in the Neuromuscular Junction of Mice

et al. 2020

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Western societies are facing a clear increase in the rate of obesity and overweight which are responsible for musculoskeletal pain. Some of the substances described in the environment of myofascial trigger points (MTrPs) are the same as those found in the skeletal muscle of obese people, such as cytokines. Furthermore, elevated neuromuscular neurotransmission has been associated with MTrPs. The main objective of this study is to assess whether obesity or overweight may be a facilitator of myofascial pain. The experiments were performed on male Swiss mice. One experimental group was given a typical “cafeteria” diet and another group a commercial high-fat diet for six weeks. Intramuscular adipocytes were assessed with Sudan III. The functional study was performed with electromyographic recording to determine the plaque noise and intracellular recording of miniature endplate potentials (MEPPs). The intake of a cafeteria diet showed the presence of more adipocytes in muscle tissue, but not with the fat-supplemented diet. Both experimental groups showed an increase in the plaque noise and an increase in the frequency of MEPPs that lasted several weeks after interrupting diets. In summary, the supply of a hypercaloric diet for six weeks in mice increases spontaneous neurotransmission, thus facilitating the development of MTrPs.

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“…Moreover, early life is a critical period for metabolic development. Microbiota disruption during the early life could lead to changes in body composition, which is associated with increased risk of overweight status and metabolic disorders in childhood . The role of the gut microbiota in obesity was initially identified by the findings that, conventionalization of adult germ‐free mice with microbiota harvested from the cecum of normal healthy mice led to in body fat content increase and additional metabolic capabilities and of adult germ‐free mice.…”

Section: Introductionmentioning

confidence: 99%

Chlorophyll Supplementation in Early Life Prevents Diet‐Induced Obesity and Modulates Gut Microbiota in Mice

Cui

et al. 2019

Molecular Nutrition Food Res

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Scope: The high rates of overweight and obesity, currently occurring in children, underline the urgent need for preventive strategies in early life before excess weight is gained. Methods and results: In this study, the alleviating obesity property of chlorophyll supplementation in early life is investigated using a 4-week-old C57BL/6J male mice model with obesity induced by high-fat diet (HFD). The present study finds that chlorophyll supplementation in early life can effectively retard body weight gain, improve glucose tolerance, as well as reduce low-grade inflammation in HFD-fed mice. Consequently, high-throughput sequencing of the 16S rRNA demonstrates that chlorophyll supplementation significantly reversed the HFD-induced gut dysbiosis, as evidenced by the decreased Firmicutes-to-Bacteroidetes ratios as chlorophyll is introduced. Furthermore, in chlorophyll-fed mice, the increased abundance of Blautia and norank f Bacteroidales S24-7 group, and the decreased abundance of Lactococcus and Lactobacillus, bring about the possibility that chlorophyll's alterations to the gut microbiota composition would be the main factors for dietary chlorophyll alleviating obesity-related indexes. Conclusion: Taken together, these results reveal that the intake of chlorophyll in early life may propel healthy weight management and produce beneficial effects against potential obesity in later life.

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Sex differences in the development of hepatic steatosis in cafeteria diet-induced obesity in young mice

Cited by 46 publications

References 98 publications

Sex Dimorphism in the Fgf21 Gene Expression in Liver and Adipose Tissues is Dependent on the Metabolic Condition

Sex Dimorphism in the Fgf21 Gene Expression in Liver and Adipose Tissues is Dependent on the Metabolic Condition

Effects of a Fat-Rich Diet on the Spontaneous Release of Acetylcholine in the Neuromuscular Junction of Mice

Chlorophyll Supplementation in Early Life Prevents Diet‐Induced Obesity and Modulates Gut Microbiota in Mice

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